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The map label for this gene is grpE [H]

Identifier: 148260644

GI number: 148260644

Start: 1820326

End: 1820934

Strand: Reverse

Name: grpE [H]

Synonym: Acry_1647

Alternate gene names: 148260644

Gene position: 1820934-1820326 (Counterclockwise)

Preceding gene: 148260646

Following gene: 148260643

Centisome position: 53.73

GC content: 72.09

Gene sequence:

>609_bases
ATGACCGAAGACACGCTGAACGCCGAACCCGTGCTCGACGCGGGGGCGGATCCCTTGGAGGCCGGCAGCGAGAGGGCGCC
CGCTTCCGAGGCCGAGCAGCTTGCCGCCGCCCGCGAGCGGATCGCCGCCCTCGAGGCGGAGTCCGCCGATCTGCGGGACA
AATGGGTGCGCGCGCAGGCGGAGATGGAGAACCTGCGGGCCCGCACGCGGCGCGAGGTGGAGGATGCGCGGCTCTACGCC
GTGCAGAAATTCGCCGCCGACGTTGCCGAGACCGCCGAGAACCTTCGCCGCGGGCTGGATGCGCTGCCGCCGCCGCAGGA
GGGCGAGTCGCCACTGCTCGCCAGGCTGCGCGACGGCTTCGCCGGGGTGGAGCGCAGCTTCATCGCCATGCTCGAACGCC
ACGGCATCCGCGCCGAGGAGGCGATGGGCGCGACATTCGACGCCGACAAGCACCAGGCGATGGGCGAGCAGGAGACATCC
GATGCCCCGCCGGGCACGGTGATCCAGGCCTGGAGCCGCACCTGGACGCTGAACGGTCGCCTGCTCAAGCCGGCGATGGT
CGTCGTCGCCAGGGCGCAGGCGGGCAAGCCGCTGGATATCGAGGCGTGA

Upstream 100 bases:

>100_bases
CGCCCAAAATTAAAAATTGTAATCGTTGCGGCTTTCGTTCCGCGGCTATCCGACCGCGCCGGCCCTTGTGCAGGGTCGGG
CCGTGTCCTAATTGCGGCGC

Downstream 100 bases:

>100_bases
GCCTTGAGTTTTCGTTAACGCCGGGCGGCTAAATTTCCGCAGCCGCCGGCCGGCCGCCCCTTGTGCCTTCGGGGGTGGTT
CAATACATCAACGGGGTGAA

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTEDTLNAEPVLDAGADPLEAGSERAPASEAEQLAAARERIAALEAESADLRDKWVRAQAEMENLRARTRREVEDARLYA
VQKFAADVAETAENLRRGLDALPPPQEGESPLLARLRDGFAGVERSFIAMLERHGIRAEEAMGATFDADKHQAMGEQETS
DAPPGTVIQAWSRTWTLNGRLLKPAMVVVARAQAGKPLDIEA

Sequences:

>Translated_202_residues
MTEDTLNAEPVLDAGADPLEAGSERAPASEAEQLAAARERIAALEAESADLRDKWVRAQAEMENLRARTRREVEDARLYA
VQKFAADVAETAENLRRGLDALPPPQEGESPLLARLRDGFAGVERSFIAMLERHGIRAEEAMGATFDADKHQAMGEQETS
DAPPGTVIQAWSRTWTLNGRLLKPAMVVVARAQAGKPLDIEA
>Mature_201_residues
TEDTLNAEPVLDAGADPLEAGSERAPASEAEQLAAARERIAALEAESADLRDKWVRAQAEMENLRARTRREVEDARLYAV
QKFAADVAETAENLRRGLDALPPPQEGESPLLARLRDGFAGVERSFIAMLERHGIRAEEAMGATFDADKHQAMGEQETSD
APPGTVIQAWSRTWTLNGRLLKPAMVVVARAQAGKPLDIEA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=166, Percent_Identity=31.9277108433735, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI40255109, Length=158, Percent_Identity=29.746835443038, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788967, Length=172, Percent_Identity=31.9767441860465, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17552458, Length=152, Percent_Identity=30.9210526315789, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=164, Percent_Identity=29.2682926829268, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653432, Length=192, Percent_Identity=27.0833333333333, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21983; Mature: 21852

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDTLNAEPVLDAGADPLEAGSERAPASEAEQLAAARERIAALEAESADLRDKWVRAQA
CCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EMENLRARTRREVEDARLYAVQKFAADVAETAENLRRGLDALPPPQEGESPLLARLRDGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AGVERSFIAMLERHGIRAEEAMGATFDADKHQAMGEQETSDAPPGTVIQAWSRTWTLNGR
HHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHEEECCCE
LLKPAMVVVARAQAGKPLDIEA
EHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TEDTLNAEPVLDAGADPLEAGSERAPASEAEQLAAARERIAALEAESADLRDKWVRAQA
CCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EMENLRARTRREVEDARLYAVQKFAADVAETAENLRRGLDALPPPQEGESPLLARLRDGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AGVERSFIAMLERHGIRAEEAMGATFDADKHQAMGEQETSDAPPGTVIQAWSRTWTLNGR
HHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHEEECCCE
LLKPAMVVVARAQAGKPLDIEA
EHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA