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The map label for this gene is cysS

Identifier: 148260406

GI number: 148260406

Start: 1559131

End: 1560480

Strand: Reverse

Name: cysS

Synonym: Acry_1404

Alternate gene names: 148260406

Gene position: 1560480-1559131 (Counterclockwise)

Preceding gene: 148260411

Following gene: 148260405

Centisome position: 46.04

GC content: 68.67

Gene sequence:

>1350_bases
ATGATCCAGATCAAGCTGCACAACACGAAAACACGCCGGCGCGAGCCCTTCGCCCCCGCCGACCCCGCGCATGTGAAGCT
CTATGTCTGCGGTCCCACGGTCTATGACCGCGCGCATCTCGGCAATGCGCGCACGGTGGTGGTGTTCGACACGCTGGTCC
GCCTGCTGCGCCACCTGTTCCCGCGCGTCACCTATGTGCGGAACATCACCGACATCGATGACAAGATCAACGCCCGCGCC
GCCGAAACCGGCGAGACGATCGGCGAGATCACCGCGCGGACGACAAGCTGGTTCCACGAGGACATGGCCGCCCTGTACTG
CGCCCCGCCCGATATCGAGCCGCGCGCCACCGGGCATATCGGCGACATCATCGCCCTCATCGAGCGCCTGATCGCCCGCG
GCCACGCCTATGCCGCCGAGGGCCATGTCCTGTTCGCCGTCGCGACCGATGCGGAATACGGCAAGTTTTCCGGCCGCTCG
CCCGAGGAACTGCTGGCCGGCGCGCGGGTGGACGTCGCCACCTACAAGCGCGATCCGGGCGATTTCGTGCTCTGGAAACC
CTCGCCGCCCGACCTGCCGGGCTGGGACAGCCCCTGGGGCCGCGGCCGGCCGGGCTGGCACATCGAATGCTCGGCGATGA
TCCACGCCACCCTCGGCGAGACGATCGACATCCATGGCGGCGGCGCCGACCTGATCTTCCCGCATCATGAGAACGAGATC
GCCCAGTCCTGCTGCGCCTTTCCCGGCTCGGAGTTCGCCCGCGTCTGGGTGCATGCCGGCATGTTGCAGGTGGACGGGCA
GAAAATGTCGAAATCCCTCGGCAATTTCCGCACTGTGCAGGACGTGCTGGGCGAGGCGCCGGGCGAGGCGGTGCGCTTCC
TGCTGCTCAAGACGCATTATCGCGGCGTGCTTGACTTCTCCACCGCCGCGCTGGCCGAAGCGAAGCGGGAGCTCGACCGG
TTCTACCGCGCATTGGAAAAGCATGCCGACCCCGCGCCCGCCGCCACACCGCCCGCCGCCTTCATCGAGGCCCTGGCGGA
TGACCTGAACACCCCCGGCGCGATTGCCGAACTCCACGCGCTGGCCGATGCGGCGATGCAGGGCGATGCCGCCAGCGCCG
CCGGCCTGCGCGCCGCCGGCATGCTGATCGGCATTTTCAATCACACTGCCGACCAGTGGTTCCGCGGTGAGGCGACCGAC
GATGCGCGGATCGACGCGCTGATCGCCGAGCGTCTGGCCGCGCGGAGGAACAAGGATTTCGCCCGCGCCGACGCGATCCG
CGCCGAACTCGCCGCCGCCGGCATCCTGCTCGAGGACGGTCCCGGCGGTACCACCTGGCGGCGCGCATGA

Upstream 100 bases:

>100_bases
AGAATTATTCTCAACTGATGGTCATGGTGAATACAGGACCTCACAGGTCCTGTCAACGCGCCGGTTGACTTCCGCTTGCC
CGGAGAGGAAATCCGCGGCC

Downstream 100 bases:

>100_bases
CCGGGCGCGATGAAGGCGCTGATCTCGCGCCGATCGACCCGTCCCCCGTCATCATCCTGGTCCGCCCGCAGCTTGCCGAG
AATATCGGCTCGGTCGCGCG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MIQIKLHNTKTRRREPFAPADPAHVKLYVCGPTVYDRAHLGNARTVVVFDTLVRLLRHLFPRVTYVRNITDIDDKINARA
AETGETIGEITARTTSWFHEDMAALYCAPPDIEPRATGHIGDIIALIERLIARGHAYAAEGHVLFAVATDAEYGKFSGRS
PEELLAGARVDVATYKRDPGDFVLWKPSPPDLPGWDSPWGRGRPGWHIECSAMIHATLGETIDIHGGGADLIFPHHENEI
AQSCCAFPGSEFARVWVHAGMLQVDGQKMSKSLGNFRTVQDVLGEAPGEAVRFLLLKTHYRGVLDFSTAALAEAKRELDR
FYRALEKHADPAPAATPPAAFIEALADDLNTPGAIAELHALADAAMQGDAASAAGLRAAGMLIGIFNHTADQWFRGEATD
DARIDALIAERLAARRNKDFARADAIRAELAAAGILLEDGPGGTTWRRA

Sequences:

>Translated_449_residues
MIQIKLHNTKTRRREPFAPADPAHVKLYVCGPTVYDRAHLGNARTVVVFDTLVRLLRHLFPRVTYVRNITDIDDKINARA
AETGETIGEITARTTSWFHEDMAALYCAPPDIEPRATGHIGDIIALIERLIARGHAYAAEGHVLFAVATDAEYGKFSGRS
PEELLAGARVDVATYKRDPGDFVLWKPSPPDLPGWDSPWGRGRPGWHIECSAMIHATLGETIDIHGGGADLIFPHHENEI
AQSCCAFPGSEFARVWVHAGMLQVDGQKMSKSLGNFRTVQDVLGEAPGEAVRFLLLKTHYRGVLDFSTAALAEAKRELDR
FYRALEKHADPAPAATPPAAFIEALADDLNTPGAIAELHALADAAMQGDAASAAGLRAAGMLIGIFNHTADQWFRGEATD
DARIDALIAERLAARRNKDFARADAIRAELAAAGILLEDGPGGTTWRRA
>Mature_449_residues
MIQIKLHNTKTRRREPFAPADPAHVKLYVCGPTVYDRAHLGNARTVVVFDTLVRLLRHLFPRVTYVRNITDIDDKINARA
AETGETIGEITARTTSWFHEDMAALYCAPPDIEPRATGHIGDIIALIERLIARGHAYAAEGHVLFAVATDAEYGKFSGRS
PEELLAGARVDVATYKRDPGDFVLWKPSPPDLPGWDSPWGRGRPGWHIECSAMIHATLGETIDIHGGGADLIFPHHENEI
AQSCCAFPGSEFARVWVHAGMLQVDGQKMSKSLGNFRTVQDVLGEAPGEAVRFLLLKTHYRGVLDFSTAALAEAKRELDR
FYRALEKHADPAPAATPPAAFIEALADDLNTPGAIAELHALADAAMQGDAASAAGLRAAGMLIGIFNHTADQWFRGEATD
DARIDALIAERLAARRNKDFARADAIRAELAAAGILLEDGPGGTTWRRA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=503, Percent_Identity=34.7912524850895, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI21269877, Length=241, Percent_Identity=39.0041493775934, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI10835051, Length=241, Percent_Identity=39.0041493775934, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI62240992, Length=241, Percent_Identity=39.0041493775934, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI303304987, Length=241, Percent_Identity=39.0041493775934, Blast_Score=186, Evalue=5e-47,
Organism=Escherichia coli, GI1786737, Length=460, Percent_Identity=50, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17509797, Length=236, Percent_Identity=38.5593220338983, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17509795, Length=236, Percent_Identity=38.5593220338983, Blast_Score=168, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6324082, Length=319, Percent_Identity=32.2884012539185, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI19922218, Length=377, Percent_Identity=36.3395225464191, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI19922364, Length=241, Percent_Identity=39.8340248962656, Blast_Score=182, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ACICJ (A5FYD2)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001234533.1
- ProteinModelPortal:   A5FYD2
- SMR:   A5FYD2
- STRING:   A5FYD2
- GeneID:   5160514
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_1404
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- ProtClustDB:   PRK00260
- BioCyc:   ACRY349163:ACRY_1404-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 48864; Mature: 48864

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIKLHNTKTRRREPFAPADPAHVKLYVCGPTVYDRAHLGNARTVVVFDTLVRLLRHLF
CEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCCCCEEEHHHHHHHHHHHHH
PRVTYVRNITDIDDKINARAAETGETIGEITARTTSWFHEDMAALYCAPPDIEPRATGHI
HHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCH
GDIIALIERLIARGHAYAAEGHVLFAVATDAEYGKFSGRSPEELLAGARVDVATYKRDPG
HHHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHCCCCEEEEEECCCCC
DFVLWKPSPPDLPGWDSPWGRGRPGWHIECSAMIHATLGETIDIHGGGADLIFPHHENEI
CEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHEEEECCCCEEEECCCCEEEECCCCCHHH
AQSCCAFPGSEFARVWVHAGMLQVDGQKMSKSLGNFRTVQDVLGEAPGEAVRFLLLKTHY
HHHHHCCCCHHHHHHHHHHCCEEECHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHH
RGVLDFSTAALAEAKRELDRFYRALEKHADPAPAATPPAAFIEALADDLNTPGAIAELHA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LADAAMQGDAASAAGLRAAGMLIGIFNHTADQWFRGEATDDARIDALIAERLAARRNKDF
HHHHHHCCCCHHHHHHHHHHHHEEHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCH
ARADAIRAELAAAGILLEDGPGGTTWRRA
HHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MIQIKLHNTKTRRREPFAPADPAHVKLYVCGPTVYDRAHLGNARTVVVFDTLVRLLRHLF
CEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCCCCEEEHHHHHHHHHHHHH
PRVTYVRNITDIDDKINARAAETGETIGEITARTTSWFHEDMAALYCAPPDIEPRATGHI
HHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCH
GDIIALIERLIARGHAYAAEGHVLFAVATDAEYGKFSGRSPEELLAGARVDVATYKRDPG
HHHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHCCCCEEEEEECCCCC
DFVLWKPSPPDLPGWDSPWGRGRPGWHIECSAMIHATLGETIDIHGGGADLIFPHHENEI
CEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHEEEECCCCEEEECCCCEEEECCCCCHHH
AQSCCAFPGSEFARVWVHAGMLQVDGQKMSKSLGNFRTVQDVLGEAPGEAVRFLLLKTHY
HHHHHCCCCHHHHHHHHHHCCEEECHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHH
RGVLDFSTAALAEAKRELDRFYRALEKHADPAPAATPPAAFIEALADDLNTPGAIAELHA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LADAAMQGDAASAAGLRAAGMLIGIFNHTADQWFRGEATDDARIDALIAERLAARRNKDF
HHHHHHCCCCHHHHHHHHHHHHEEHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCH
ARADAIRAELAAAGILLEDGPGGTTWRRA
HHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA