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The map label for this gene is trmJ [H]

Identifier: 148260405

GI number: 148260405

Start: 1558361

End: 1559134

Strand: Reverse

Name: trmJ [H]

Synonym: Acry_1403

Alternate gene names: 148260405

Gene position: 1559134-1558361 (Counterclockwise)

Preceding gene: 148260406

Following gene: 148260404

Centisome position: 46.0

GC content: 69.12

Gene sequence:

>774_bases
ATGACCGGGCGCGATGAAGGCGCTGATCTCGCGCCGATCGACCCGTCCCCCGTCATCATCCTGGTCCGCCCGCAGCTTGC
CGAGAATATCGGCTCGGTCGCGCGGGCAATGGGCAATGGCGGGCTGTTCCATCTCCGCCTCGTCGCTCCGCGCGACGGCT
GGCCGCAGGACCGGGCGATGCGCACCGCCTCCGGCGCCTACCGCATCCTCGAAGCCGCGACGGTGCATGACACGGTGGCC
GACGCGGTGGGCGACCTGCACCGCGTCTTCGCCACCTGTCCGCGCCCGCGCCACATCGTCAAGCCGCTGCTCACCGCCCG
CGGTGCCGCCGCCGAACTGCGCGAGATCGGCGCTCGCGGCCTGAAATGCGGCATCCTGTTCGGCCCCGAGCGCGCCGGCC
TCGACAATGACGACATCGCCTGCGCCGACAGCCTGATCCGCTACCCGCTCAACCCGCGCTTCATGTCCCTCAACCTCGCC
CAGGCGGTGATGGTGATGGCCTATGAATGGTGGATCGCCGGCGAGGCCGCGCCGGTCCGCACGCTGCAGACCCACAAGAC
CCATGTCGCGACCAAGGGCGAGCTGGAAAACTTCCTCACCCACCTGATCGCCGAATGCGACGAGGCCGGTTTCCTGCGCA
ACGAGCAGAAACGGCCCGGCATGGTGCGCAACATCCGCCACTTCTTCCAGCGCGGCGAGGTGACGGAACAGGAATTGCGC
ACGCTGCATGGCATCGTCACCGAACTTGCGCGCGGCCGCCGTGGCGCGCGTTGA

Upstream 100 bases:

>100_bases
GGAACAAGGATTTCGCCCGCGCCGACGCGATCCGCGCCGAACTCGCCGCCGCCGGCATCCTGCTCGAGGACGGTCCCGGC
GGTACCACCTGGCGGCGCGC

Downstream 100 bases:

>100_bases
GTTGGCGTCCCGATTCTGTCATCATAAGGGCGCCATATCGAAGCGGCGATCAGCGGAGTGGACAGCGTGATGGACCAGGT
CGAAAGCGCAGCGCCGAACG

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTGRDEGADLAPIDPSPVIILVRPQLAENIGSVARAMGNGGLFHLRLVAPRDGWPQDRAMRTASGAYRILEAATVHDTVA
DAVGDLHRVFATCPRPRHIVKPLLTARGAAAELREIGARGLKCGILFGPERAGLDNDDIACADSLIRYPLNPRFMSLNLA
QAVMVMAYEWWIAGEAAPVRTLQTHKTHVATKGELENFLTHLIAECDEAGFLRNEQKRPGMVRNIRHFFQRGEVTEQELR
TLHGIVTELARGRRGAR

Sequences:

>Translated_257_residues
MTGRDEGADLAPIDPSPVIILVRPQLAENIGSVARAMGNGGLFHLRLVAPRDGWPQDRAMRTASGAYRILEAATVHDTVA
DAVGDLHRVFATCPRPRHIVKPLLTARGAAAELREIGARGLKCGILFGPERAGLDNDDIACADSLIRYPLNPRFMSLNLA
QAVMVMAYEWWIAGEAAPVRTLQTHKTHVATKGELENFLTHLIAECDEAGFLRNEQKRPGMVRNIRHFFQRGEVTEQELR
TLHGIVTELARGRRGAR
>Mature_256_residues
TGRDEGADLAPIDPSPVIILVRPQLAENIGSVARAMGNGGLFHLRLVAPRDGWPQDRAMRTASGAYRILEAATVHDTVAD
AVGDLHRVFATCPRPRHIVKPLLTARGAAAELREIGARGLKCGILFGPERAGLDNDDIACADSLIRYPLNPRFMSLNLAQ
AVMVMAYEWWIAGEAAPVRTLQTHKTHVATKGELENFLTHLIAECDEAGFLRNEQKRPGMVRNIRHFFQRGEVTEQELRT
LHGIVTELARGRRGAR

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=32.7868852459016, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1790865, Length=157, Percent_Identity=35.031847133758, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28305; Mature: 28174

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRDEGADLAPIDPSPVIILVRPQLAENIGSVARAMGNGGLFHLRLVAPRDGWPQDRAM
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
RTASGAYRILEAATVHDTVADAVGDLHRVFATCPRPRHIVKPLLTARGAAAELREIGARG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCC
LKCGILFGPERAGLDNDDIACADSLIRYPLNPRFMSLNLAQAVMVMAYEWWIAGEAAPVR
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHH
TLQTHKTHVATKGELENFLTHLIAECDEAGFLRNEQKRPGMVRNIRHFFQRGEVTEQELR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH
TLHGIVTELARGRRGAR
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TGRDEGADLAPIDPSPVIILVRPQLAENIGSVARAMGNGGLFHLRLVAPRDGWPQDRAM
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
RTASGAYRILEAATVHDTVADAVGDLHRVFATCPRPRHIVKPLLTARGAAAELREIGARG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCC
LKCGILFGPERAGLDNDDIACADSLIRYPLNPRFMSLNLAQAVMVMAYEWWIAGEAAPVR
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHH
TLQTHKTHVATKGELENFLTHLIAECDEAGFLRNEQKRPGMVRNIRHFFQRGEVTEQELR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH
TLHGIVTELARGRRGAR
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA