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The map label for this gene is nuoN2

Identifier: 148260112

GI number: 148260112

Start: 1233728

End: 1235188

Strand: Reverse

Name: nuoN2

Synonym: Acry_1108

Alternate gene names: 148260112

Gene position: 1235188-1233728 (Counterclockwise)

Preceding gene: 148260113

Following gene: 148260111

Centisome position: 36.44

GC content: 70.5

Gene sequence:

>1461_bases
ATGACCGCCCCGGCCTTCACCGCCAAGGCCTTCGCCGCCTTCGCGCCCTTCACCCTGCTCGGCGCGGTGACGATCCTGGT
CATGCTGCTGATCGCCGTCCGCCGCGATCACCGCCTCGTCGCCCTGTCGACCATCGCCGGCCTGCTGCTCTGCTGCATCG
CCCTGCCGCTCGCCGCTCCCGCATCCCCCGTCGCCGTGACGAAGCTGTTCGTGATCGACGGCGCCGCCCTGTTCTATGCG
GGCCTCGTGCTCGTCTCCGCCCTGGTCGTCGCCGCGCTCTCCTGGGTCTATCTCGACCGGGTGGGCGAGGGGCCGCGCGA
GGAATACTATCTCCTCCTGCTCACCGCGACGCTGGGCGCCGCCATCCTGCCGGCGAGCGTGCATTTCGCCTCGTTCTTCC
TCGGCATCGAAACGCTCGGCATCTCGCTGCTCGGGCTGATCGCCTATGCCGGCCCGCGCGGCAGCGCGGCCGAGGCGGGC
ACGAAATATCTCATCCTCAGCGGCGCCGCCTCCGCCTTCCTGCTGTTCGGCATGGCGCTGATCTATGCCGCCTTCGGCTC
GATGTCCTTCGCCCGCATCGGCGCGCTCGCCCATCAAGCCGGCCCGGCCGATCTCTACCGCCTCGCCGGCATCGCGCTCA
TTCTCTCCGGCGTCGCCTTCAAGCTCTCGCTGGTCCCGTTCCACATGTGGACGCCCGACGTGTACGAGGGCGCGCCCGCG
CCGGTCACCGCCTTCATCGCCGTCGTCTCGAAAATCGCGGTCTTCGCCGTGATCCTGCGCTTCTTCGCCCTCGCCGGCGC
TCATGCGGATGCCTCGCTCCGCGCCGGCATCGCCGCGGTCGCGATCCTGTCCATGCTCGCCGGCAACCTGCTCGCCCTGC
TGCAGACCAACGTGAAGCGCCTGCTCGCCTATTCGTCCATCGCCCATCTGGGCTACCTGCTCGTCGCCTTCCTCGCAGCC
CCGGCGCTTGGCGTCGATGCCGCGGCCTATTACCTGGCCGCCTACGCGGTCACCAGCCTCGGCGCCTTCGGCATCGTCGC
GCTGCTCAGCGCGCCGGGCGCGGGGCGCGATGCCGACCGGATCGAGGATTACCGCGGCCTGTTCCGCACCCGCCCGTTCC
TCGCCGCGAGCCTCGCGCTGATGCTGCTCTCGCTCGCGGGCATGCCGCCGACGATGGGCTTCATCGCCAAGTTCTACCTC
GTCGTCGCCGGCGTCGGCACCGCCCTGTTCTGGCCGGTCGCCGCACTCCTGCTCGGCAGCGTGATCGGCCTCTACTACTA
TCTGCGCATCCTCGTCGCGATGGCGCTGCCCGCCCCCGGCGATGAGGAGGCGCGACATCCGAACGCCGTGCCCCCGCTCG
GCAACGCGGTGATCGGCGCGCTGCTCGTCCTGCTGGTGGTGCTCGGCGCCTATCCGGCGCCCGCCCTCGCGCTGATCCGC
ATGACCATCGGCCACGGCTGA

Upstream 100 bases:

>100_bases
CGCAGCCCGTCTTCGACGAGGTGGCGCCGGGCCTCTCCGCAGTGCAGCACAGCGCCGCGGTCTCGGCGGCGCTGCCCGAG
CACCTCGCGGAGCGCGCGCC

Downstream 100 bases:

>100_bases
CCGCCGCCACCGCCGGCCGTCATGGCCATGCGCCCTACCGCTGTGCATCCTGCATGGCTGGTCACCTCGCCGCCGCCTCA
AAATGCTGGCATCGGGACGG

Product: proton-translocating NADH-quinone oxidoreductase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N 2; NDH-1 subunit N 2

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTAPAFTAKAFAAFAPFTLLGAVTILVMLLIAVRRDHRLVALSTIAGLLLCCIALPLAAPASPVAVTKLFVIDGAALFYA
GLVLVSALVVAALSWVYLDRVGEGPREEYYLLLLTATLGAAILPASVHFASFFLGIETLGISLLGLIAYAGPRGSAAEAG
TKYLILSGAASAFLLFGMALIYAAFGSMSFARIGALAHQAGPADLYRLAGIALILSGVAFKLSLVPFHMWTPDVYEGAPA
PVTAFIAVVSKIAVFAVILRFFALAGAHADASLRAGIAAVAILSMLAGNLLALLQTNVKRLLAYSSIAHLGYLLVAFLAA
PALGVDAAAYYLAAYAVTSLGAFGIVALLSAPGAGRDADRIEDYRGLFRTRPFLAASLALMLLSLAGMPPTMGFIAKFYL
VVAGVGTALFWPVAALLLGSVIGLYYYLRILVAMALPAPGDEEARHPNAVPPLGNAVIGALLVLLVVLGAYPAPALALIR
MTIGHG

Sequences:

>Translated_486_residues
MTAPAFTAKAFAAFAPFTLLGAVTILVMLLIAVRRDHRLVALSTIAGLLLCCIALPLAAPASPVAVTKLFVIDGAALFYA
GLVLVSALVVAALSWVYLDRVGEGPREEYYLLLLTATLGAAILPASVHFASFFLGIETLGISLLGLIAYAGPRGSAAEAG
TKYLILSGAASAFLLFGMALIYAAFGSMSFARIGALAHQAGPADLYRLAGIALILSGVAFKLSLVPFHMWTPDVYEGAPA
PVTAFIAVVSKIAVFAVILRFFALAGAHADASLRAGIAAVAILSMLAGNLLALLQTNVKRLLAYSSIAHLGYLLVAFLAA
PALGVDAAAYYLAAYAVTSLGAFGIVALLSAPGAGRDADRIEDYRGLFRTRPFLAASLALMLLSLAGMPPTMGFIAKFYL
VVAGVGTALFWPVAALLLGSVIGLYYYLRILVAMALPAPGDEEARHPNAVPPLGNAVIGALLVLLVVLGAYPAPALALIR
MTIGHG
>Mature_485_residues
TAPAFTAKAFAAFAPFTLLGAVTILVMLLIAVRRDHRLVALSTIAGLLLCCIALPLAAPASPVAVTKLFVIDGAALFYAG
LVLVSALVVAALSWVYLDRVGEGPREEYYLLLLTATLGAAILPASVHFASFFLGIETLGISLLGLIAYAGPRGSAAEAGT
KYLILSGAASAFLLFGMALIYAAFGSMSFARIGALAHQAGPADLYRLAGIALILSGVAFKLSLVPFHMWTPDVYEGAPAP
VTAFIAVVSKIAVFAVILRFFALAGAHADASLRAGIAAVAILSMLAGNLLALLQTNVKRLLAYSSIAHLGYLLVAFLAAP
ALGVDAAAYYLAAYAVTSLGAFGIVALLSAPGAGRDADRIEDYRGLFRTRPFLAASLALMLLSLAGMPPTMGFIAKFYLV
VAGVGTALFWPVAALLLGSVIGLYYYLRILVAMALPAPGDEEARHPNAVPPLGNAVIGALLVLLVVLGAYPAPALALIRM
TIGHG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Escherichia coli, GI145693160, Length=482, Percent_Identity=46.0580912863071, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1788831, Length=277, Percent_Identity=28.5198555956679, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788827, Length=366, Percent_Identity=28.1420765027322, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788613, Length=339, Percent_Identity=23.8938053097345, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788829, Length=394, Percent_Identity=25.6345177664975, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788614, Length=209, Percent_Identity=29.6650717703349, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON2_ACICJ (A5FXI8)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001234239.1
- STRING:   A5FXI8
- GeneID:   5160991
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_1108
- HOGENOM:   HBG747830
- OMA:   ALTQSNL
- BioCyc:   ACRY349163:ACRY_1108-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 50182; Mature: 50050

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x144791f4)-; HASH(0x143aa028)-; HASH(0x145338e8)-; HASH(0x13cded58)-; HASH(0x14615bd4)-; HASH(0x141674ac)-; HASH(0x137958dc)-; HASH(0x13cd2210)-; HASH(0x117c2af0)-; HASH(0x13ebbbb4)-; HASH(0x13d4ae3c)-; HASH(0x13dfa780)-; HASH(0x1481b79c)-; HASH(0x1458b28c)-; HASH(0x14535b7c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPAFTAKAFAAFAPFTLLGAVTILVMLLIAVRRDHRLVALSTIAGLLLCCIALPLAAP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
ASPVAVTKLFVIDGAALFYAGLVLVSALVVAALSWVYLDRVGEGPREEYYLLLLTATLGA
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHH
AILPASVHFASFFLGIETLGISLLGLIAYAGPRGSAAEAGTKYLILSGAASAFLLFGMAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEEECHHHHHHHHHHHH
IYAAFGSMSFARIGALAHQAGPADLYRLAGIALILSGVAFKLSLVPFHMWTPDVYEGAPA
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCCCCC
PVTAFIAVVSKIAVFAVILRFFALAGAHADASLRAGIAAVAILSMLAGNLLALLQTNVKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAYSSIAHLGYLLVAFLAAPALGVDAAAYYLAAYAVTSLGAFGIVALLSAPGAGRDADR
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
IEDYRGLFRTRPFLAASLALMLLSLAGMPPTMGFIAKFYLVVAGVGTALFWPVAALLLGS
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIGLYYYLRILVAMALPAPGDEEARHPNAVPPLGNAVIGALLVLLVVLGAYPAPALALIR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
MTIGHG
HHHCCH
>Mature Secondary Structure 
TAPAFTAKAFAAFAPFTLLGAVTILVMLLIAVRRDHRLVALSTIAGLLLCCIALPLAAP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
ASPVAVTKLFVIDGAALFYAGLVLVSALVVAALSWVYLDRVGEGPREEYYLLLLTATLGA
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHH
AILPASVHFASFFLGIETLGISLLGLIAYAGPRGSAAEAGTKYLILSGAASAFLLFGMAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEEECHHHHHHHHHHHH
IYAAFGSMSFARIGALAHQAGPADLYRLAGIALILSGVAFKLSLVPFHMWTPDVYEGAPA
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCCCCC
PVTAFIAVVSKIAVFAVILRFFALAGAHADASLRAGIAAVAILSMLAGNLLALLQTNVKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAYSSIAHLGYLLVAFLAAPALGVDAAAYYLAAYAVTSLGAFGIVALLSAPGAGRDADR
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
IEDYRGLFRTRPFLAASLALMLLSLAGMPPTMGFIAKFYLVVAGVGTALFWPVAALLLGS
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIGLYYYLRILVAMALPAPGDEEARHPNAVPPLGNAVIGALLVLLVVLGAYPAPALALIR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
MTIGHG
HHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA