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The map label for this gene is nuoM [H]

Identifier: 148260113

GI number: 148260113

Start: 1235185

End: 1236702

Strand: Reverse

Name: nuoM [H]

Synonym: Acry_1109

Alternate gene names: 148260113

Gene position: 1236702-1235185 (Counterclockwise)

Preceding gene: 148260114

Following gene: 148260112

Centisome position: 36.49

GC content: 67.98

Gene sequence:

>1518_bases
ATGATCCTGTTCTGGCTCCTCATCTTCCCGGCGGTCGGCGGCGTGCTGGCCTGGATCGCCGGCACCCGCAACCCGGATGC
CCCGCGCTGGGTGTCGATCGCGGTGCTCACGCTCGAACTTCTCATGGCGCTCCCCCTGATCGGCGCCGCCGCCGCACCCG
GCCATGGCGCGTGGATCGCGCAGTTCGACCACTCGTGGATTCCGCAGCTCGGCATCTCCTTCGAACTCGATCTCGACGGG
CTGAGCCTGGTCCTGATCCTGCTGACGATCGTGCTCAGCCTCATCTGCGTCGTGATCTCCTGGACCGAGATCACCGACCG
TTCCGGCCTGTTCCACGCCAACCTGCTCTGGACCACCACCGGCGTCATCGGCGTCTTCCTCGCGCTCGACCTGTTCCTGT
TCTTCTTCTTCTGGGAACTGATGCTGGTGCCGATGTATTTCATGATCGCGCTATGGGGCCACGAGAACCGCTCCTACGCG
GCGATCAAGTTCTTCCTCTTCACCCAGGGCAGCGGCCTGCTCATGCTGGTGTCGATCCTCGTGCTCGTCTTCATCCACCA
GCGCGCGACCGGCGATCTCACCTTCGACTATTTCGCCCTGCTCGGCACGAAAATGAGCCCGACCACCGGCTTCCTGCTGA
TGCTCGGCTTCTTCATCGCCTTCGCCGTGAAGCTGCCGGCGGTGCCGTTCCACACCTGGCTGCCGGACGCCCACACCGAA
GCCCCGACCGCGGGTTCCGTGCTGCTCGCCGGCATCCTGCTGAAGACCGGCGCCTACGGCCTGATCCGCTTCGTGGTGCC
GCTGTTTCCCGCGGCGGCGCACGCCTTCGCGCCGGTGGCGATGGCGCTCGCGGTCGCCGGCATCCTCTACGGCGCGCTGC
TCGCCTTCGGCCAGAACGACCTCAAGCGCCTGGTCGCCTACAGCAGCATCAGCCACATGGGCTTCGTCCTGCTCGGCGTC
TTCGCCTGGAGCACGCTCGCCCTGCAGGGCGTGGTCGCGCAGATGGTGGCGCACGGCATCAGCACCGGCGCCCTCTTCGT
CCTGGTCGGCGCCCTGCAGGAACGCATCCACACCCGCGACATGCGGCGCATGGGCGGGCTCTGGGGGCAGGTGCCGCGCC
TGTCCGCCTGCGGCATGTTCTTCGCCATCGCCTCGCTCGGCCTTCCCGGCCTCGGCAATTTCATCGGTGAATTCCTCATC
CTGCTCGGCAGCTTCCAGGCCAGTGTGGTCTTCACCGTCATCGCCACGTTCGGGCTGGTCGCCGCCGCCGCCTATTCGCT
GCTGCTGGTCCAGCGCGCCTTCCACGGCGCGGCGCAGGAACCGGCACCCCATCGCGCGATCGCCGATCTCTCGGCGCCGC
ATCTCGCCGTCATGGCGGTGCTGGTGGTCGCCATCGTCGGGCTCGGCCTCTATCCGCAGCCCGTCTTCGACGAGGTGGCG
CCGGGCCTCTCCGCAGTGCAGCACAGCGCCGCGGTCTCGGCGGCGCTGCCCGAGCACCTCGCGGAGCGCGCGCCATGA

Upstream 100 bases:

>100_bases
TCCACCGGGCGATCCGGCGCACCCAGACCGGCCGGGTCCGCCTCTATGCCGCGAGCCTCGCCATCGGCTCGGTCGGCCTG
ATCGCGATCGCGGTGCTGCG

Downstream 100 bases:

>100_bases
CCGCCCCGGCCTTCACCGCCAAGGCCTTCGCCGCCTTCGCGCCCTTCACCCTGCTCGGCGCGGTGACGATCCTGGTCATG
CTGCTGATCGCCGTCCGCCG

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MILFWLLIFPAVGGVLAWIAGTRNPDAPRWVSIAVLTLELLMALPLIGAAAAPGHGAWIAQFDHSWIPQLGISFELDLDG
LSLVLILLTIVLSLICVVISWTEITDRSGLFHANLLWTTTGVIGVFLALDLFLFFFFWELMLVPMYFMIALWGHENRSYA
AIKFFLFTQGSGLLMLVSILVLVFIHQRATGDLTFDYFALLGTKMSPTTGFLLMLGFFIAFAVKLPAVPFHTWLPDAHTE
APTAGSVLLAGILLKTGAYGLIRFVVPLFPAAAHAFAPVAMALAVAGILYGALLAFGQNDLKRLVAYSSISHMGFVLLGV
FAWSTLALQGVVAQMVAHGISTGALFVLVGALQERIHTRDMRRMGGLWGQVPRLSACGMFFAIASLGLPGLGNFIGEFLI
LLGSFQASVVFTVIATFGLVAAAAYSLLLVQRAFHGAAQEPAPHRAIADLSAPHLAVMAVLVVAIVGLGLYPQPVFDEVA
PGLSAVQHSAAVSAALPEHLAERAP

Sequences:

>Translated_505_residues
MILFWLLIFPAVGGVLAWIAGTRNPDAPRWVSIAVLTLELLMALPLIGAAAAPGHGAWIAQFDHSWIPQLGISFELDLDG
LSLVLILLTIVLSLICVVISWTEITDRSGLFHANLLWTTTGVIGVFLALDLFLFFFFWELMLVPMYFMIALWGHENRSYA
AIKFFLFTQGSGLLMLVSILVLVFIHQRATGDLTFDYFALLGTKMSPTTGFLLMLGFFIAFAVKLPAVPFHTWLPDAHTE
APTAGSVLLAGILLKTGAYGLIRFVVPLFPAAAHAFAPVAMALAVAGILYGALLAFGQNDLKRLVAYSSISHMGFVLLGV
FAWSTLALQGVVAQMVAHGISTGALFVLVGALQERIHTRDMRRMGGLWGQVPRLSACGMFFAIASLGLPGLGNFIGEFLI
LLGSFQASVVFTVIATFGLVAAAAYSLLLVQRAFHGAAQEPAPHRAIADLSAPHLAVMAVLVVAIVGLGLYPQPVFDEVA
PGLSAVQHSAAVSAALPEHLAERAP
>Mature_505_residues
MILFWLLIFPAVGGVLAWIAGTRNPDAPRWVSIAVLTLELLMALPLIGAAAAPGHGAWIAQFDHSWIPQLGISFELDLDG
LSLVLILLTIVLSLICVVISWTEITDRSGLFHANLLWTTTGVIGVFLALDLFLFFFFWELMLVPMYFMIALWGHENRSYA
AIKFFLFTQGSGLLMLVSILVLVFIHQRATGDLTFDYFALLGTKMSPTTGFLLMLGFFIAFAVKLPAVPFHTWLPDAHTE
APTAGSVLLAGILLKTGAYGLIRFVVPLFPAAAHAFAPVAMALAVAGILYGALLAFGQNDLKRLVAYSSISHMGFVLLGV
FAWSTLALQGVVAQMVAHGISTGALFVLVGALQERIHTRDMRRMGGLWGQVPRLSACGMFFAIASLGLPGLGNFIGEFLI
LLGSFQASVVFTVIATFGLVAAAAYSLLLVQRAFHGAAQEPAPHRAIADLSAPHLAVMAVLVVAIVGLGLYPQPVFDEVA
PGLSAVQHSAAVSAALPEHLAERAP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=327, Percent_Identity=33.0275229357798, Blast_Score=162, Evalue=9e-40,
Organism=Escherichia coli, GI1788613, Length=498, Percent_Identity=53.8152610441767, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1788827, Length=358, Percent_Identity=26.536312849162, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788829, Length=410, Percent_Identity=28.0487804878049, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI145693160, Length=420, Percent_Identity=27.1428571428571, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788831, Length=200, Percent_Identity=31.5, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788614, Length=388, Percent_Identity=27.5773195876289, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54025; Mature: 54025

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFWLLIFPAVGGVLAWIAGTRNPDAPRWVSIAVLTLELLMALPLIGAAAAPGHGAWIA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
QFDHSWIPQLGISFELDLDGLSLVLILLTIVLSLICVVISWTEITDRSGLFHANLLWTTT
ECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
GVIGVFLALDLFLFFFFWELMLVPMYFMIALWGHENRSYAAIKFFLFTQGSGLLMLVSIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHH
VLVFIHQRATGDLTFDYFALLGTKMSPTTGFLLMLGFFIAFAVKLPAVPFHTWLPDAHTE
HHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCC
APTAGSVLLAGILLKTGAYGLIRFVVPLFPAAAHAFAPVAMALAVAGILYGALLAFGQND
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LKRLVAYSSISHMGFVLLGVFAWSTLALQGVVAQMVAHGISTGALFVLVGALQERIHTRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
MRRMGGLWGQVPRLSACGMFFAIASLGLPGLGNFIGEFLILLGSFQASVVFTVIATFGLV
HHHHCCHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAYSLLLVQRAFHGAAQEPAPHRAIADLSAPHLAVMAVLVVAIVGLGLYPQPVFDEVA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHC
PGLSAVQHSAAVSAALPEHLAERAP
CCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILFWLLIFPAVGGVLAWIAGTRNPDAPRWVSIAVLTLELLMALPLIGAAAAPGHGAWIA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
QFDHSWIPQLGISFELDLDGLSLVLILLTIVLSLICVVISWTEITDRSGLFHANLLWTTT
ECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
GVIGVFLALDLFLFFFFWELMLVPMYFMIALWGHENRSYAAIKFFLFTQGSGLLMLVSIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHH
VLVFIHQRATGDLTFDYFALLGTKMSPTTGFLLMLGFFIAFAVKLPAVPFHTWLPDAHTE
HHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCC
APTAGSVLLAGILLKTGAYGLIRFVVPLFPAAAHAFAPVAMALAVAGILYGALLAFGQND
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LKRLVAYSSISHMGFVLLGVFAWSTLALQGVVAQMVAHGISTGALFVLVGALQERIHTRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
MRRMGGLWGQVPRLSACGMFFAIASLGLPGLGNFIGEFLILLGSFQASVVFTVIATFGLV
HHHHCCHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAYSLLLVQRAFHGAAQEPAPHRAIADLSAPHLAVMAVLVVAIVGLGLYPQPVFDEVA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHC
PGLSAVQHSAAVSAALPEHLAERAP
CCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]