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The map label for this gene is livK [C]

Identifier: 148260060

GI number: 148260060

Start: 1172812

End: 1174065

Strand: Reverse

Name: livK [C]

Synonym: Acry_1052

Alternate gene names: 148260060

Gene position: 1174065-1172812 (Counterclockwise)

Preceding gene: 148260061

Following gene: 148260059

Centisome position: 34.64

GC content: 65.47

Gene sequence:

>1254_bases
ATGGATTTCGAGAACTCTGCATCGTTCAAGCGTCGCGGGCTGCTGAAGGCTGGCGCCGCCGCCGGCGTGTCCAGCCTTGT
CCTCCCGCAAGTCAGCTGGGGCGCCAGCGAAACGCCGGTCAAGATCGGCATGATCGACCCGCGCACCGGCGTCTACGCGC
CTGCGGGTCACAACGAGATCATCGGCGCCACCATGGCGGTCGACGAGATCAACAAGAAGGGCGGCATTCTCGGCCGGCCC
GTCCAGCTTCTCGTCGAGGACGACAACAGCCTGCCCGGGCCTGCCGTCAGCAAGGCGCAGAAGCTCGTCGCCGAGGACAA
GGTGAACTTCCTGATGGGCACGGTGAATTCGGCCGCGTCCGAATCGCTCAGCCAGTTCGCCCTTCAGCACGAGATGCTGT
TCGTCGACACCGGCGGCCACGCGGATGACGTGACCGGCAGCAAGTGCCACTGGACCACGTTCCGCACCTGCTCGACCACC
TGGATGCTCACCGCCGGCGACTTCGAGACGCTCTTCAAGAAGTTCGGCAAGAAGTGGTTCTTCATCGTGCCCGACTATGC
CTTCGGTCACGCCCTCTACAAGGACTACATGATCCAGCTGAAGAAGGCGGGCGGCCAGGCCGCGGGCCACGCGCTCGCGC
CGCTGGGCACCACCGACTTCTCCTCCTACCTGATCCGCGCCCGCGCCGCGAAGCCGGACGTGCTGATCTGCCTGCAGGCG
GGCGACGACCTCGTGAACCTGCTGAAGCAGGCCGTGCAGTTCGGCCTCGACAAGCAGATGGCGATCGCCGGCGCGCTGCA
GGAAATGGAAGTGCTCCAGGCGCTGCCGAAGGCCGCTCTCCTCGGCTGGTGGACCTTCGAGTGGTACTGGAACCAGCCCG
GCGTTCCGCATGTCGCCGATTTCGTGACGGCGGTGAAGAAGCGCACCGGCGGCAAGGTGCCGACGGCGCGAACCTGGTTC
GGCTATGCCACCGCCCACGCCATCGCGATGGCCGCGAACAATGCCAAGTCGCTCGACTCGCTGAAGGTGGTTAAGGCCCT
CGAGGGCATGGAACTGCCGCCGGATGTGGCACTGCAGCCGGGCAAGGTTTACTACCGCGCCGGGGACCATCAGATGATGG
CGAACATGTTCCCCGGCTACGTCCCCAAGGACGCGAGCTACCCGAACCTGTTCAAGGTGGCCGAAGTGGTGCCGAGCGAG
AAGATCGCCCTGTCGCCGGAGGCCGCTGGCTGCAAGATGACGTACCCGAGCTGA

Upstream 100 bases:

>100_bases
CCGTAAGATCCTCGTGTTAGTAGAATGCGGACCGCGCCTGATCGCTGGGCCGAATGCGTCGTGTGAAGCGGCCACAAGTA
CGGAAGGGGAAATGATCCAA

Downstream 100 bases:

>100_bases
AGCCCCGCCCGCGTGGCGGACTGGACCGGTCCACGGCGAAAGCCGTGGGCCCGTTCGCCGCATCCGCCGCGCGACCTCGT
CTCCAGCCGCGCCCGCGGGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MDFENSASFKRRGLLKAGAAAGVSSLVLPQVSWGASETPVKIGMIDPRTGVYAPAGHNEIIGATMAVDEINKKGGILGRP
VQLLVEDDNSLPGPAVSKAQKLVAEDKVNFLMGTVNSAASESLSQFALQHEMLFVDTGGHADDVTGSKCHWTTFRTCSTT
WMLTAGDFETLFKKFGKKWFFIVPDYAFGHALYKDYMIQLKKAGGQAAGHALAPLGTTDFSSYLIRARAAKPDVLICLQA
GDDLVNLLKQAVQFGLDKQMAIAGALQEMEVLQALPKAALLGWWTFEWYWNQPGVPHVADFVTAVKKRTGGKVPTARTWF
GYATAHAIAMAANNAKSLDSLKVVKALEGMELPPDVALQPGKVYYRAGDHQMMANMFPGYVPKDASYPNLFKVAEVVPSE
KIALSPEAAGCKMTYPS

Sequences:

>Translated_417_residues
MDFENSASFKRRGLLKAGAAAGVSSLVLPQVSWGASETPVKIGMIDPRTGVYAPAGHNEIIGATMAVDEINKKGGILGRP
VQLLVEDDNSLPGPAVSKAQKLVAEDKVNFLMGTVNSAASESLSQFALQHEMLFVDTGGHADDVTGSKCHWTTFRTCSTT
WMLTAGDFETLFKKFGKKWFFIVPDYAFGHALYKDYMIQLKKAGGQAAGHALAPLGTTDFSSYLIRARAAKPDVLICLQA
GDDLVNLLKQAVQFGLDKQMAIAGALQEMEVLQALPKAALLGWWTFEWYWNQPGVPHVADFVTAVKKRTGGKVPTARTWF
GYATAHAIAMAANNAKSLDSLKVVKALEGMELPPDVALQPGKVYYRAGDHQMMANMFPGYVPKDASYPNLFKVAEVVPSE
KIALSPEAAGCKMTYPS
>Mature_417_residues
MDFENSASFKRRGLLKAGAAAGVSSLVLPQVSWGASETPVKIGMIDPRTGVYAPAGHNEIIGATMAVDEINKKGGILGRP
VQLLVEDDNSLPGPAVSKAQKLVAEDKVNFLMGTVNSAASESLSQFALQHEMLFVDTGGHADDVTGSKCHWTTFRTCSTT
WMLTAGDFETLFKKFGKKWFFIVPDYAFGHALYKDYMIQLKKAGGQAAGHALAPLGTTDFSSYLIRARAAKPDVLICLQA
GDDLVNLLKQAVQFGLDKQMAIAGALQEMEVLQALPKAALLGWWTFEWYWNQPGVPHVADFVTAVKKRTGGKVPTARTWF
GYATAHAIAMAANNAKSLDSLKVVKALEGMELPPDVALQPGKVYYRAGDHQMMANMFPGYVPKDASYPNLFKVAEVVPSE
KIALSPEAAGCKMTYPS

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=254, Percent_Identity=24.0157480314961, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 45025; Mature: 45025

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFENSASFKRRGLLKAGAAAGVSSLVLPQVSWGASETPVKIGMIDPRTGVYAPAGHNEI
CCCCCCCCHHHHCCHHHHHHHCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
IGATMAVDEINKKGGILGRPVQLLVEDDNSLPGPAVSKAQKLVAEDKVNFLMGTVNSAAS
EEHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLSQFALQHEMLFVDTGGHADDVTGSKCHWTTFRTCSTTWMLTAGDFETLFKKFGKKWF
HHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEEEEECCCEEEEECCCHHHHHHHHCCEEE
FIVPDYAFGHALYKDYMIQLKKAGGQAAGHALAPLGTTDFSSYLIRARAAKPDVLICLQA
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCEEEEEEC
GDDLVNLLKQAVQFGLDKQMAIAGALQEMEVLQALPKAALLGWWTFEWYWNQPGVPHVAD
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCHHHH
FVTAVKKRTGGKVPTARTWFGYATAHAIAMAANNAKSLDSLKVVKALEGMELPPDVALQP
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
GKVYYRAGDHQMMANMFPGYVPKDASYPNLFKVAEVVPSEKIALSPEAAGCKMTYPS
CEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCEECCCC
>Mature Secondary Structure
MDFENSASFKRRGLLKAGAAAGVSSLVLPQVSWGASETPVKIGMIDPRTGVYAPAGHNEI
CCCCCCCCHHHHCCHHHHHHHCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
IGATMAVDEINKKGGILGRPVQLLVEDDNSLPGPAVSKAQKLVAEDKVNFLMGTVNSAAS
EEHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLSQFALQHEMLFVDTGGHADDVTGSKCHWTTFRTCSTTWMLTAGDFETLFKKFGKKWF
HHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEEEEECCCEEEEECCCHHHHHHHHCCEEE
FIVPDYAFGHALYKDYMIQLKKAGGQAAGHALAPLGTTDFSSYLIRARAAKPDVLICLQA
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCEEEEEEC
GDDLVNLLKQAVQFGLDKQMAIAGALQEMEVLQALPKAALLGWWTFEWYWNQPGVPHVAD
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCHHHH
FVTAVKKRTGGKVPTARTWFGYATAHAIAMAANNAKSLDSLKVVKALEGMELPPDVALQP
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
GKVYYRAGDHQMMANMFPGYVPKDASYPNLFKVAEVVPSEKIALSPEAAGCKMTYPS
CEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA