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The map label for this gene is trmB [H]

Identifier: 148260061

GI number: 148260061

Start: 1174273

End: 1174977

Strand: Reverse

Name: trmB [H]

Synonym: Acry_1053

Alternate gene names: 148260061

Gene position: 1174977-1174273 (Counterclockwise)

Preceding gene: 148260062

Following gene: 148260060

Centisome position: 34.67

GC content: 67.8

Gene sequence:

>705_bases
TTGGCCGCTGAGGAGACGGTTGAGGAGACGGCGGGCGGCAAGGCGCTGAAGCCGCCGCCCGACCGGCTCTATGGCCGCGC
CCGCGGCCACGCGCTGCGGGCGCGCCAGGAGGAGCTGATCGAGACGTTGCTGCCGCGCCTGCGCTGGCCGGAGCGGAACT
TCGCCATCGCGCCGAAGGAACTCTGGCTCGAGGTCGGCGCCGGCGGGTTTGAGCATGCCGAGGCGATCGCCGCGGCCAAT
CCCGAAATCGGCCTTGTCGCCTGCGAGGTGTTCGTCAACTGCATCGCCTCGCTGCTCTCGCGCCTCGCCCCCGAGGGTGT
CGAGCCGACAATCCCGCCCAACCTGCGCGTGCACGACGACGACGCCCGCGCCCTGCTGCGCACCCTGCCGGCGGGATCGG
TCTCGCGCCTGTTCCTGATGTTCCCCGATCCCTGGCCGAAGGCGCGCCACGCGAAGCGGCGGTTCGTCCATCCGGCGATC
CTCGCCGAGGTCGCCCGCGTGCTCCGGCCGGGCGGGCTGTGGCGGATCGCCAGCGACGACCCGACCTATCAACAATGGGT
TGAGGAAGTATTTTCTAACGCAGAGGCATTTTCTTTGTTAAGTAAATCCGGAATTCGCCCCCCGGACTGGCCGCCGACCC
GATATGAAGCCAAGGCCATCAAGGCAGGGCGCCAGCCGCACTACTGGACATTTGTTGTAAAATAG

Upstream 100 bases:

>100_bases
CCGACCTCCGCCTACGGCCATTTCGGCCGCGAGCCGGACGACCGGCTGGGCACCTTCACCTGGGAGAAGACCGACCTCGC
GCCGGCGCTGAAGGCCGCGT

Downstream 100 bases:

>100_bases
CCTGTCAATTCGACGTTTTACGACCTCCGGGCTGTTGACAGATCAGGTATGCAGGATAAAACCGGAATGTGACGTGCGGT
TGCCACGATATAACCGGGGT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MAAEETVEETAGGKALKPPPDRLYGRARGHALRARQEELIETLLPRLRWPERNFAIAPKELWLEVGAGGFEHAEAIAAAN
PEIGLVACEVFVNCIASLLSRLAPEGVEPTIPPNLRVHDDDARALLRTLPAGSVSRLFLMFPDPWPKARHAKRRFVHPAI
LAEVARVLRPGGLWRIASDDPTYQQWVEEVFSNAEAFSLLSKSGIRPPDWPPTRYEAKAIKAGRQPHYWTFVVK

Sequences:

>Translated_234_residues
MAAEETVEETAGGKALKPPPDRLYGRARGHALRARQEELIETLLPRLRWPERNFAIAPKELWLEVGAGGFEHAEAIAAAN
PEIGLVACEVFVNCIASLLSRLAPEGVEPTIPPNLRVHDDDARALLRTLPAGSVSRLFLMFPDPWPKARHAKRRFVHPAI
LAEVARVLRPGGLWRIASDDPTYQQWVEEVFSNAEAFSLLSKSGIRPPDWPPTRYEAKAIKAGRQPHYWTFVVK
>Mature_233_residues
AAEETVEETAGGKALKPPPDRLYGRARGHALRARQEELIETLLPRLRWPERNFAIAPKELWLEVGAGGFEHAEAIAAANP
EIGLVACEVFVNCIASLLSRLAPEGVEPTIPPNLRVHDDDARALLRTLPAGSVSRLFLMFPDPWPKARHAKRRFVHPAIL
AEVARVLRPGGLWRIASDDPTYQQWVEEVFSNAEAFSLLSKSGIRPPDWPPTRYEAKAIKAGRQPHYWTFVVK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=165, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26129; Mature: 25998

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAEETVEETAGGKALKPPPDRLYGRARGHALRARQEELIETLLPRLRWPERNFAIAPKE
CCCHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LWLEVGAGGFEHAEAIAAANPEIGLVACEVFVNCIASLLSRLAPEGVEPTIPPNLRVHDD
HHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCH
DARALLRTLPAGSVSRLFLMFPDPWPKARHAKRRFVHPAILAEVARVLRPGGLWRIASDD
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCC
PTYQQWVEEVFSNAEAFSLLSKSGIRPPDWPPTRYEAKAIKAGRQPHYWTFVVK
CHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
AAEETVEETAGGKALKPPPDRLYGRARGHALRARQEELIETLLPRLRWPERNFAIAPKE
CCHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LWLEVGAGGFEHAEAIAAANPEIGLVACEVFVNCIASLLSRLAPEGVEPTIPPNLRVHDD
HHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCH
DARALLRTLPAGSVSRLFLMFPDPWPKARHAKRRFVHPAILAEVARVLRPGGLWRIASDD
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCC
PTYQQWVEEVFSNAEAFSLLSKSGIRPPDWPPTRYEAKAIKAGRQPHYWTFVVK
CHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA