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The map label for this gene is stcD [H]

Identifier: 148260009

GI number: 148260009

Start: 1108227

End: 1110263

Strand: Reverse

Name: stcD [H]

Synonym: Acry_1000

Alternate gene names: 148260009

Gene position: 1110263-1108227 (Counterclockwise)

Preceding gene: 148260010

Following gene: 148260008

Centisome position: 32.76

GC content: 70.05

Gene sequence:

>2037_bases
ATGATCGCCCACGCCGAGGCGCTGTTCCGCCCGCTGACGATCCGCCGGACGACGTTCCGCAACCGGATCATGAGCACGAG
CCACGCGCCAGGCTACGGCCGCGACGGCAAGCCGCAGGAGCGCTACCAGCTCTACCACGCGGAAAAGGCGAAGGGCGGCA
TCGGGCTGACGATGTTCGGCGGATCGTCCTCCGTCTCGCTCGACAGCCCGGCGGCGCCGTGGAACCAGATATCGGTCGCG
GATGACAGCGTCATTCCGTATTTTCAACAATTTTCCGAACGCGTGCACGCGCATGGCGCGGCGCTGATGATCCAGCTCAC
GCATATGGGGCGGCGGACCCGCTGGGATACCGAGAACTGGCTGCCGACGCTTTCCGCCTCGCCGCGGCGGGAGCCGGCGA
GCCGGTCGATCCCGCGGGAGATGGAGCAGGAGGATATCGACCGGGTGGTCGCCGATTTCGCGGCGGCGGCGAGGCGGGCG
CGCGAGGGCGGGCTCGATGGCTGCGAACTCTCGGCGGCGCACGGGCACCTGGTCGACCAGTTCTGGTCGCCCTCGGTCAA
TCATCGCGGCGATGGTTATGGCGGGTCGCTGGAGAACCGGATGCGGTTCGGCTTCGAGATTCTCGAGGCGATGCGGGCGG
CGGCGGGGGATGATTTCCTGATCGGCATCCGCATGTCGGGCGACGAGATGCTGGCCGAGGGGCTGAGCCACGAGGACTGC
GTGGCCATCGCCGAGGCGCATGCGCGGCGCGGGCTGGTCGATTTCATCAACGTGCTCGGCGGGCAGGCGCGGGACCATCT
GGCGCATGCGGTGTCGCTGCCGAACATGTCGTTTCCGGTCGCGCCCTTCCTGCCGCTGGCCAGCGCGATCCGCCGCGAGG
TGGAGGTGCCGGTGTTCCACGCGCAGCGGGTCACCGACCTTGCCACCGCCGCGCGCGCGGTGGCGGAGGGACATGTCGAC
ATGATCGCGATGACCCGGGCGCACATCGCCGACCCGCATCTCGCGCGCAAGCTGCAGGACGGGCGGGAGGACGATATCCG
CCAGTGCGTCGGCGCCGGCTACTGCATCGACCGGATCTATACCGGGGCGGATGCGCTGTGCATCCAGAACGCCGCGACCG
GGCGCGAGGCGACGATGCCGCACGTGATCGGCAAGGCGGCGCGGCGGCGGCGGGTGGTTGTCGTCGGCGGCGGGCCGGGC
GGGCTGGAGGCGGCGCGGGTGAGTGCGGAGCGCGGCCACGAGGTGGTGCTGTTCGAGCGGGAGACGGCGCTTGGCGGGCA
GATCAACATCGCCGCGAAGGCGGGATGGCGGGAGGCGCTGTCGGGGATTCCGCGCTGGCTCGGCGCGCAGGTGCGCAGGC
TCGGCGTGGATCTGCGGCTCGGTGTCGCGGCGGATGCGGCGATGGTGCTGGCGGAACGGCCGGACATCGTGATTCTCGCG
ACCGGCGGCGTGCCCAATCCGGGCTTCATGGAGGGGGCGGAGCTGGTGACGACGACCGCCGCGATCCTGCGCGACGGGAT
CGCGCTCAGCGGTTCGGTGCTGCTCTATGACGGGATGGGGCAGCACAACGCCGCCTCCTGCGCCGAATTCCTGGCGAAGC
GCGGCGCGCTGGTGGAACTCGCGACCGGCGACCGGATGGCGGCCGAGGAGGTCGGCACCACCAACCAGCCGATCCATCTG
CGCGAACTCTACCGCCATGACGTGATCATGACGCCGAATCTCGACCTGCTCGCGGTGCGGCGGGAGGAGAACCGGCTGGT
CGCGGTGCTGCGCAACACGATGGATCTCGCCGAGGAGGAGCGGGTGGTGGATCATGTCGTCGTCGATTTCGGCACGCTGC
CGGATGACGCGCTTTATGATCCGCTGAAGGCGGGGTCGGTCAACCGGGGGCGGATCGATGCCGGCGCGCTGGTGGCGGGG
CGGGCGCAGCCCTGGCCGGAGGACGGATACGCGCTGTATCGCATCGGCGATGCGGCGGCGGGACGCAACATCCACGCCGC
GATCTACGACGCGCTGCGCCTCTGCAAGGATTTCTGA

Upstream 100 bases:

>100_bases
CGATGGCCTGCGGGTTCGGCTCCAGCACGCATTTCGCCAGCGCCTATCACGATTATTTCGGACATCCGCCGCGCGCCGAG
CGGCGGCCGGGAGGCATTGC

Downstream 100 bases:

>100_bases
TGGCGGGCCTGTTCGTCCTGCTGGCCTGGCTGCTGCTGGCCGGGGTGGTCGCGCAGGGGCTGCGTCTCGCCGCATCGTGG
CGGCGGGGCGGGGCGGCGCC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MIAHAEALFRPLTIRRTTFRNRIMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFGGSSSVSLDSPAAPWNQISVA
DDSVIPYFQQFSERVHAHGAALMIQLTHMGRRTRWDTENWLPTLSASPRREPASRSIPREMEQEDIDRVVADFAAAARRA
REGGLDGCELSAAHGHLVDQFWSPSVNHRGDGYGGSLENRMRFGFEILEAMRAAAGDDFLIGIRMSGDEMLAEGLSHEDC
VAIAEAHARRGLVDFINVLGGQARDHLAHAVSLPNMSFPVAPFLPLASAIRREVEVPVFHAQRVTDLATAARAVAEGHVD
MIAMTRAHIADPHLARKLQDGREDDIRQCVGAGYCIDRIYTGADALCIQNAATGREATMPHVIGKAARRRRVVVVGGGPG
GLEAARVSAERGHEVVLFERETALGGQINIAAKAGWREALSGIPRWLGAQVRRLGVDLRLGVAADAAMVLAERPDIVILA
TGGVPNPGFMEGAELVTTTAAILRDGIALSGSVLLYDGMGQHNAASCAEFLAKRGALVELATGDRMAAEEVGTTNQPIHL
RELYRHDVIMTPNLDLLAVRREENRLVAVLRNTMDLAEEERVVDHVVVDFGTLPDDALYDPLKAGSVNRGRIDAGALVAG
RAQPWPEDGYALYRIGDAAAGRNIHAAIYDALRLCKDF

Sequences:

>Translated_678_residues
MIAHAEALFRPLTIRRTTFRNRIMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFGGSSSVSLDSPAAPWNQISVA
DDSVIPYFQQFSERVHAHGAALMIQLTHMGRRTRWDTENWLPTLSASPRREPASRSIPREMEQEDIDRVVADFAAAARRA
REGGLDGCELSAAHGHLVDQFWSPSVNHRGDGYGGSLENRMRFGFEILEAMRAAAGDDFLIGIRMSGDEMLAEGLSHEDC
VAIAEAHARRGLVDFINVLGGQARDHLAHAVSLPNMSFPVAPFLPLASAIRREVEVPVFHAQRVTDLATAARAVAEGHVD
MIAMTRAHIADPHLARKLQDGREDDIRQCVGAGYCIDRIYTGADALCIQNAATGREATMPHVIGKAARRRRVVVVGGGPG
GLEAARVSAERGHEVVLFERETALGGQINIAAKAGWREALSGIPRWLGAQVRRLGVDLRLGVAADAAMVLAERPDIVILA
TGGVPNPGFMEGAELVTTTAAILRDGIALSGSVLLYDGMGQHNAASCAEFLAKRGALVELATGDRMAAEEVGTTNQPIHL
RELYRHDVIMTPNLDLLAVRREENRLVAVLRNTMDLAEEERVVDHVVVDFGTLPDDALYDPLKAGSVNRGRIDAGALVAG
RAQPWPEDGYALYRIGDAAAGRNIHAAIYDALRLCKDF
>Mature_678_residues
MIAHAEALFRPLTIRRTTFRNRIMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFGGSSSVSLDSPAAPWNQISVA
DDSVIPYFQQFSERVHAHGAALMIQLTHMGRRTRWDTENWLPTLSASPRREPASRSIPREMEQEDIDRVVADFAAAARRA
REGGLDGCELSAAHGHLVDQFWSPSVNHRGDGYGGSLENRMRFGFEILEAMRAAAGDDFLIGIRMSGDEMLAEGLSHEDC
VAIAEAHARRGLVDFINVLGGQARDHLAHAVSLPNMSFPVAPFLPLASAIRREVEVPVFHAQRVTDLATAARAVAEGHVD
MIAMTRAHIADPHLARKLQDGREDDIRQCVGAGYCIDRIYTGADALCIQNAATGREATMPHVIGKAARRRRVVVVGGGPG
GLEAARVSAERGHEVVLFERETALGGQINIAAKAGWREALSGIPRWLGAQVRRLGVDLRLGVAADAAMVLAERPDIVILA
TGGVPNPGFMEGAELVTTTAAILRDGIALSGSVLLYDGMGQHNAASCAEFLAKRGALVELATGDRMAAEEVGTTNQPIHL
RELYRHDVIMTPNLDLLAVRREENRLVAVLRNTMDLAEEERVVDHVVVDFGTLPDDALYDPLKAGSVNRGRIDAGALVAG
RAQPWPEDGYALYRIGDAAAGRNIHAAIYDALRLCKDF

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=664, Percent_Identity=29.5180722891566, Blast_Score=242, Evalue=6e-65,
Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=29.3150684931507, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=268, Percent_Identity=32.089552238806, Blast_Score=116, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=269, Percent_Identity=31.5985130111524, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=32.089552238806, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI72001454, Length=270, Percent_Identity=32.5925925925926, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17540738, Length=298, Percent_Identity=29.8657718120805, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=272, Percent_Identity=30.8823529411765, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17566914, Length=324, Percent_Identity=25, Blast_Score=86, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=93, Percent_Identity=34.4086021505376, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=86, Percent_Identity=38.3720930232558, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 73453; Mature: 73453

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAHAEALFRPLTIRRTTFRNRIMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFG
CCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
GSSSVSLDSPAAPWNQISVADDSVIPYFQQFSERVHAHGAALMIQLTHMGRRTRWDTENW
CCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHC
LPTLSASPRREPASRSIPREMEQEDIDRVVADFAAAARRAREGGLDGCELSAAHGHLVDQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHH
FWSPSVNHRGDGYGGSLENRMRFGFEILEAMRAAAGDDFLIGIRMSGDEMLAEGLSHEDC
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCHHH
VAIAEAHARRGLVDFINVLGGQARDHLAHAVSLPNMSFPVAPFLPLASAIRREVEVPVFH
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHH
AQRVTDLATAARAVAEGHVDMIAMTRAHIADPHLARKLQDGREDDIRQCVGAGYCIDRIY
HHHHHHHHHHHHHHHHCCHHHEEHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHH
TGADALCIQNAATGREATMPHVIGKAARRRRVVVVGGGPGGLEAARVSAERGHEVVLFER
CCCCHHEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEC
ETALGGQINIAAKAGWREALSGIPRWLGAQVRRLGVDLRLGVAADAAMVLAERPDIVILA
CCCCCCEEEEEECCCHHHHHHHCHHHHHHHHHHHCCCEEECCHHHHHHHEECCCCEEEEE
TGGVPNPGFMEGAELVTTTAAILRDGIALSGSVLLYDGMGQHNAASCAEFLAKRGALVEL
CCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCCCCCHHHHHHHHHHCCCEEEE
ATGDRMAAEEVGTTNQPIHLRELYRHDVIMTPNLDLLAVRREENRLVAVLRNTMDLAEEE
ECCCCHHHHHCCCCCCCCHHHHHHHCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHH
RVVDHVVVDFGTLPDDALYDPLKAGSVNRGRIDAGALVAGRAQPWPEDGYALYRIGDAAA
HHHHHHHHHCCCCCCHHHHCCHHCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCC
GRNIHAAIYDALRLCKDF
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAHAEALFRPLTIRRTTFRNRIMSTSHAPGYGRDGKPQERYQLYHAEKAKGGIGLTMFG
CCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
GSSSVSLDSPAAPWNQISVADDSVIPYFQQFSERVHAHGAALMIQLTHMGRRTRWDTENW
CCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHC
LPTLSASPRREPASRSIPREMEQEDIDRVVADFAAAARRAREGGLDGCELSAAHGHLVDQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHH
FWSPSVNHRGDGYGGSLENRMRFGFEILEAMRAAAGDDFLIGIRMSGDEMLAEGLSHEDC
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCHHH
VAIAEAHARRGLVDFINVLGGQARDHLAHAVSLPNMSFPVAPFLPLASAIRREVEVPVFH
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHH
AQRVTDLATAARAVAEGHVDMIAMTRAHIADPHLARKLQDGREDDIRQCVGAGYCIDRIY
HHHHHHHHHHHHHHHHCCHHHEEHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHH
TGADALCIQNAATGREATMPHVIGKAARRRRVVVVGGGPGGLEAARVSAERGHEVVLFER
CCCCHHEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEC
ETALGGQINIAAKAGWREALSGIPRWLGAQVRRLGVDLRLGVAADAAMVLAERPDIVILA
CCCCCCEEEEEECCCHHHHHHHCHHHHHHHHHHHCCCEEECCHHHHHHHEECCCCEEEEE
TGGVPNPGFMEGAELVTTTAAILRDGIALSGSVLLYDGMGQHNAASCAEFLAKRGALVEL
CCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCCCCCHHHHHHHHHHCCCEEEE
ATGDRMAAEEVGTTNQPIHLRELYRHDVIMTPNLDLLAVRREENRLVAVLRNTMDLAEEE
ECCCCHHHHHCCCCCCCCHHHHHHHCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHH
RVVDHVVVDFGTLPDDALYDPLKAGSVNRGRIDAGALVAGRAQPWPEDGYALYRIGDAAA
HHHHHHHHHCCCCCCHHHHCCHHCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCC
GRNIHAAIYDALRLCKDF
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]