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The map label for this gene is otsB [H]

Identifier: 148259877

GI number: 148259877

Start: 957395

End: 958105

Strand: Reverse

Name: otsB [H]

Synonym: Acry_0866

Alternate gene names: 148259877

Gene position: 958105-957395 (Counterclockwise)

Preceding gene: 148259878

Following gene: 148259876

Centisome position: 28.27

GC content: 75.81

Gene sequence:

>711_bases
ATGGGACCGAAGCTGACGCGCGATCACGCGCTGTTCCTCGATCTGGACGGCACGTTGCTGGAGTTTGCCCCGACGGCGGA
AGAGGTGGCGGTGCCCGCGGGGCTGGGGGCGATGCTGCGCGGGCTCGCGGCGCAGCGCGACGGGGCGCTGGCGATACTCT
CGGGGCGGTCGCTCGCGCAGATCGACGCGGTGACCGCGGACCCCTTCCCCGCCGGCGCCGAGCATGGGTTCGTGCTGCGC
GACGCGGCGGGGCGAATCCATCATCCGGTGCGGCAGACGGCCAACCGCGCGGCCTGGCGGGCGGCGCTGGAGGCGGCGGC
GGCGGCGCATCCGGGCGTGCGGATCGAGGCGAAATCGGCCTCGCTGGTCGCGCATTACCGGGCGTCACCCGAGGCGGGGC
CGGCGCTGGGCGCGATGATCGGCGCGATGATCGCGGAGGCGCCGGAGGTCGAGCGGCTGGCGGCGAACATGGCCTGGGAG
ATCCGCCCGCGCGGCGCGGGCAAGGGGCGCGCGCTGGAATGGTTCATGGCGCGGCCGCCTTTCGCCGGGCGCGTGCCGGT
CTTCGTCGGCGACGATGTGACCGACGAGGAGGCGATCGCGGCGGCGGCGGCGCTGGGCGGGCACGGGCTGCACGTTCACC
GCGATTTCGACGGCACGCCGGCCGCGGTGCTGGCCTGGCTGGGCAGCGCGCTGGCGCCGGGGAGGGTGTGA

Upstream 100 bases:

>100_bases
GCATCACCTGATGTCGTGGCCGGGGGCGCGGCGGTAGGCTTCCGCCGCCGGACCGGCTTCCTTGGTTGATCGGGGCATCA
GCCATGCCCATTTCTCCCGG

Downstream 100 bases:

>100_bases
GGATGGGGCGGCTCGTCGTCGTTTCGAACCGGGTGTCGCTGCCGACCGACAAGGGCGCGAGGGCCGGCGGGCTGGCCGTG
GCGCTGGAGGACGCGATGAT

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MGPKLTRDHALFLDLDGTLLEFAPTAEEVAVPAGLGAMLRGLAAQRDGALAILSGRSLAQIDAVTADPFPAGAEHGFVLR
DAAGRIHHPVRQTANRAAWRAALEAAAAAHPGVRIEAKSASLVAHYRASPEAGPALGAMIGAMIAEAPEVERLAANMAWE
IRPRGAGKGRALEWFMARPPFAGRVPVFVGDDVTDEEAIAAAAALGGHGLHVHRDFDGTPAAVLAWLGSALAPGRV

Sequences:

>Translated_236_residues
MGPKLTRDHALFLDLDGTLLEFAPTAEEVAVPAGLGAMLRGLAAQRDGALAILSGRSLAQIDAVTADPFPAGAEHGFVLR
DAAGRIHHPVRQTANRAAWRAALEAAAAAHPGVRIEAKSASLVAHYRASPEAGPALGAMIGAMIAEAPEVERLAANMAWE
IRPRGAGKGRALEWFMARPPFAGRVPVFVGDDVTDEEAIAAAAALGGHGLHVHRDFDGTPAAVLAWLGSALAPGRV
>Mature_235_residues
GPKLTRDHALFLDLDGTLLEFAPTAEEVAVPAGLGAMLRGLAAQRDGALAILSGRSLAQIDAVTADPFPAGAEHGFVLRD
AAGRIHHPVRQTANRAAWRAALEAAAAAHPGVRIEAKSASLVAHYRASPEAGPALGAMIGAMIAEAPEVERLAANMAWEI
RPRGAGKGRALEWFMARPPFAGRVPVFVGDDVTDEEAIAAAAALGGHGLHVHRDFDGTPAAVLAWLGSALAPGRV

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=226, Percent_Identity=37.6106194690265, Blast_Score=137, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 24393; Mature: 24262

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPKLTRDHALFLDLDGTLLEFAPTAEEVAVPAGLGAMLRGLAAQRDGALAILSGRSLAQ
CCCCCCCCCEEEEECCCCEEECCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCEEE
IDAVTADPFPAGAEHGFVLRDAAGRIHHPVRQTANRAAWRAALEAAAAAHPGVRIEAKSA
EEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
SLVAHYRASPEAGPALGAMIGAMIAEAPEVERLAANMAWEIRPRGAGKGRALEWFMARPP
EEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCEEEEEECCCCCCCEEEEEECCCC
FAGRVPVFVGDDVTDEEAIAAAAALGGHGLHVHRDFDGTPAAVLAWLGSALAPGRV
CCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GPKLTRDHALFLDLDGTLLEFAPTAEEVAVPAGLGAMLRGLAAQRDGALAILSGRSLAQ
CCCCCCCCEEEEECCCCEEECCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCEEE
IDAVTADPFPAGAEHGFVLRDAAGRIHHPVRQTANRAAWRAALEAAAAAHPGVRIEAKSA
EEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
SLVAHYRASPEAGPALGAMIGAMIAEAPEVERLAANMAWEIRPRGAGKGRALEWFMARPP
EEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCEEEEEECCCCCCCEEEEEECCCC
FAGRVPVFVGDDVTDEEAIAAAAALGGHGLHVHRDFDGTPAAVLAWLGSALAPGRV
CCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]