Click here to switch to the map view.

The map label for this gene is otsA [H]

Identifier: 148259876

GI number: 148259876

Start: 956019

End: 957392

Strand: Reverse

Name: otsA [H]

Synonym: Acry_0865

Alternate gene names: 148259876

Gene position: 957392-956019 (Counterclockwise)

Preceding gene: 148259877

Following gene: 148259869

Centisome position: 28.25

GC content: 69.87

Gene sequence:

>1374_bases
ATGGGGCGGCTCGTCGTCGTTTCGAACCGGGTGTCGCTGCCGACCGACAAGGGCGCGAGGGCCGGCGGGCTGGCCGTGGC
GCTGGAGGACGCGATGATCCCCGGCTCGCTCTGGTTCGGCTGGTCGGGCCGGCGGGGCGGTGCGAATTCGGACCGGGCGA
GCGTGAGCGAACATCAGGGCATCACCTATGCGACGGTGGATCTCGGCGAGACGGATTATCGCCGCTTCTATGTCGGCTTC
TCGAACGGCGCGCTGTGGCCGCTGCTGCATTACCGGACCGGGCTGATCGATTACCGGCGGGACGATTACGAGGGGTATGT
CGCGGTGAACGACCTGTTCGCCGCCCGGCTGGCGCCGCTGCTGCAGCCCGATGACGTGATCTGGATCCATGACTACCAGC
TCCTGACCCTGGCGGAGGCGCTGCGCCGGCTGGGGGTGAAGAACCGGATCGGGCTGTTCGTGCATATCCCGTTCGTGCCG
CCGGCGGTGCTGCACGTGCTGCCGGAGGCGGAGCATCTGGTGCGGGCGATGGCGGCGGCCGATCTGGTCGGCTTCCAGAC
GCATGGCGACCGGTTGAATTTCCTGGAATCGGCGGCGTCCTGCATGGAGATGCAGCGCGTGGGCGAGGACGGCTTCGTCC
ATAACGGGCACCGCACCATGACGACGGTGACGCCGGTGGGCATCGATGTCGAGGGGTTCGCCCGGGCGGCGCGGCGGGCG
GCGGGCATGACCGAGACGCGGCGGCTGATCTCCAGCCTGGTGGGGCGCGCGCTGGCGATCGGGGTGGACCGGCTGGACTA
TACCAAGGGGCTGCCCCTTCGCTTCGCCGCCTTCGGCCGGCTGTTCCTGCGCCACCCCGAGCATCTGCGCCGGATCAGCC
TGTTGCAGATCGCCGCGCGCTCGCGCGAGGATGTCGACCGGTACCAGTCGCTGCGGCGGGAGCTGGACCGGCAGGCGGGC
GAGATCAACGGCGCCTATTCGGATTTCGACTGGACGCCCGTGCGCTACATGACGCGGGCGGTGAACCGCACCACGATCGC
CGGATTCTACCGGATCGCCAGCATCGGGCTGGTCACGCCGCTGCGCGACGGCATGAACCTGGTGGCGAAGGAATACATCG
CCGCGCAGGACCCGGCGGACCCCGGCGTGCTGGTGCTCTCGCGCTTCGCCGGGGCGGCGGAGGACCTGACCGAGGCGCTG
ATCGTCAACCCGTATGACGCCGACCAGGTGGCGGATGCGATGCACACGGCGCTGCTGATGCCGCCGGAGGAGCGCGTCGC
GCGGCATGCGGCGCTGCTGGCCAAGATCCGCGAGCGGACCGCCGCCTCATTCTGCCGGGCGTTCCTCGACCAGCTGCGCC
AGGTCGAGCGCTGA

Upstream 100 bases:

>100_bases
TGGGCGGGCACGGGCTGCACGTTCACCGCGATTTCGACGGCACGCCGGCCGCGGTGCTGGCCTGGCTGGGCAGCGCGCTG
GCGCCGGGGAGGGTGTGAGG

Downstream 100 bases:

>100_bases
GGCCGCCGCCGCCGCCCGGAAATGCGCCGGCAGGGCGGCGGTGACGGCGACCGGCGGGGCGAGCGGCAGGGCGAGCGCGC
GGGCGAGCAGGCACATGCCG

Product: Alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MGRLVVVSNRVSLPTDKGARAGGLAVALEDAMIPGSLWFGWSGRRGGANSDRASVSEHQGITYATVDLGETDYRRFYVGF
SNGALWPLLHYRTGLIDYRRDDYEGYVAVNDLFAARLAPLLQPDDVIWIHDYQLLTLAEALRRLGVKNRIGLFVHIPFVP
PAVLHVLPEAEHLVRAMAAADLVGFQTHGDRLNFLESAASCMEMQRVGEDGFVHNGHRTMTTVTPVGIDVEGFARAARRA
AGMTETRRLISSLVGRALAIGVDRLDYTKGLPLRFAAFGRLFLRHPEHLRRISLLQIAARSREDVDRYQSLRRELDRQAG
EINGAYSDFDWTPVRYMTRAVNRTTIAGFYRIASIGLVTPLRDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEDLTEAL
IVNPYDADQVADAMHTALLMPPEERVARHAALLAKIRERTAASFCRAFLDQLRQVER

Sequences:

>Translated_457_residues
MGRLVVVSNRVSLPTDKGARAGGLAVALEDAMIPGSLWFGWSGRRGGANSDRASVSEHQGITYATVDLGETDYRRFYVGF
SNGALWPLLHYRTGLIDYRRDDYEGYVAVNDLFAARLAPLLQPDDVIWIHDYQLLTLAEALRRLGVKNRIGLFVHIPFVP
PAVLHVLPEAEHLVRAMAAADLVGFQTHGDRLNFLESAASCMEMQRVGEDGFVHNGHRTMTTVTPVGIDVEGFARAARRA
AGMTETRRLISSLVGRALAIGVDRLDYTKGLPLRFAAFGRLFLRHPEHLRRISLLQIAARSREDVDRYQSLRRELDRQAG
EINGAYSDFDWTPVRYMTRAVNRTTIAGFYRIASIGLVTPLRDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEDLTEAL
IVNPYDADQVADAMHTALLMPPEERVARHAALLAKIRERTAASFCRAFLDQLRQVER
>Mature_456_residues
GRLVVVSNRVSLPTDKGARAGGLAVALEDAMIPGSLWFGWSGRRGGANSDRASVSEHQGITYATVDLGETDYRRFYVGFS
NGALWPLLHYRTGLIDYRRDDYEGYVAVNDLFAARLAPLLQPDDVIWIHDYQLLTLAEALRRLGVKNRIGLFVHIPFVPP
AVLHVLPEAEHLVRAMAAADLVGFQTHGDRLNFLESAASCMEMQRVGEDGFVHNGHRTMTTVTPVGIDVEGFARAARRAA
GMTETRRLISSLVGRALAIGVDRLDYTKGLPLRFAAFGRLFLRHPEHLRRISLLQIAARSREDVDRYQSLRRELDRQAGE
INGAYSDFDWTPVRYMTRAVNRTTIAGFYRIASIGLVTPLRDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEDLTEALI
VNPYDADQVADAMHTALLMPPEERVARHAALLAKIRERTAASFCRAFLDQLRQVER

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=458, Percent_Identity=45.6331877729258, Blast_Score=387, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI115533560, Length=420, Percent_Identity=32.1428571428571, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI115533558, Length=420, Percent_Identity=32.1428571428571, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI71987012, Length=422, Percent_Identity=31.2796208530806, Blast_Score=177, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6319602, Length=467, Percent_Identity=31.2633832976445, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6323917, Length=457, Percent_Identity=25.164113785558, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323537, Length=388, Percent_Identity=26.0309278350515, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320279, Length=396, Percent_Identity=22.2222222222222, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19920676, Length=488, Percent_Identity=35.2459016393443, Blast_Score=291, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 50763; Mature: 50632

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLVVVSNRVSLPTDKGARAGGLAVALEDAMIPGSLWFGWSGRRGGANSDRASVSEHQG
CCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHCC
ITYATVDLGETDYRRFYVGFSNGALWPLLHYRTGLIDYRRDDYEGYVAVNDLFAARLAPL
CEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHC
LQPDDVIWIHDYQLLTLAEALRRLGVKNRIGLFVHIPFVPPAVLHVLPEAEHLVRAMAAA
CCCCCEEEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHH
DLVGFQTHGDRLNFLESAASCMEMQRVGEDGFVHNGHRTMTTVTPVGIDVEGFARAARRA
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHH
AGMTETRRLISSLVGRALAIGVDRLDYTKGLPLRFAAFGRLFLRHPEHLRRISLLQIAAR
CCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHH
SREDVDRYQSLRRELDRQAGEINGAYSDFDWTPVRYMTRAVNRTTIAGFYRIASIGLVTP
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEDLTEALIVNPYDADQVADAMHTALLM
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHEECCCCHHHHHHHHHHHHCC
PPEERVARHAALLAKIRERTAASFCRAFLDQLRQVER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GRLVVVSNRVSLPTDKGARAGGLAVALEDAMIPGSLWFGWSGRRGGANSDRASVSEHQG
CCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHCC
ITYATVDLGETDYRRFYVGFSNGALWPLLHYRTGLIDYRRDDYEGYVAVNDLFAARLAPL
CEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHC
LQPDDVIWIHDYQLLTLAEALRRLGVKNRIGLFVHIPFVPPAVLHVLPEAEHLVRAMAAA
CCCCCEEEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHH
DLVGFQTHGDRLNFLESAASCMEMQRVGEDGFVHNGHRTMTTVTPVGIDVEGFARAARRA
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHH
AGMTETRRLISSLVGRALAIGVDRLDYTKGLPLRFAAFGRLFLRHPEHLRRISLLQIAAR
CCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHH
SREDVDRYQSLRRELDRQAGEINGAYSDFDWTPVRYMTRAVNRTTIAGFYRIASIGLVTP
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEDLTEALIVNPYDADQVADAMHTALLM
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHEECCCCHHHHHHHHHHHHCC
PPEERVARHAALLAKIRERTAASFCRAFLDQLRQVER
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA