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The map label for this gene is dmlA [H]

Identifier: 148259660

GI number: 148259660

Start: 729508

End: 730572

Strand: Reverse

Name: dmlA [H]

Synonym: Acry_0647

Alternate gene names: 148259660

Gene position: 730572-729508 (Counterclockwise)

Preceding gene: 148259661

Following gene: 148259656

Centisome position: 21.56

GC content: 69.48

Gene sequence:

>1065_bases
ATGTCGCGTCGCCACCGCATCGCCGTCATCGAGGGTGACGGCATCGGTCGCGAGGTGATCCCGGCGGCCCGCACGGTCCT
CGACCGTGCGGCCTCCGCCTGCGGCTTCACGCTCGACTGGCGCGATTTCGACTGGAGCTGCGAAACCTGGAAGGCCACCG
GGCGGATGATGCCGGAAGACGGCATCGCCCGCCTCGCCGACCATGACGCGATCTTTCTCGGCGCGGTCGGCTATCCCGGC
GTGCCGGACCACATCTCCCTCTGGGGCCTGCTGATCCCGATCCGCCGCGAGTTCGACCAGTATGTGAACCTCCGCCCGGT
CCGCCTGCTGCCCGGCGTGCCCTGCCCGCTCGCCAACAAGCAGCCCGGCGACATCGATTTCTGGGTGGTGCGCGAGAATG
TCGAGGGCGAATACAGCCAGATCGGCGGGCGGATGTTCGCCGGCACCGAGCGCGAGTTCGTCGAGCAGACCGCGATCTTC
ACCCGCGCCGGCACCGACCGCATCCTGCGCTACGCCTTCGACCTCGCCCGCCGCCTCGGCCGCCCGCACGTCACCTCGGC
GACCAAGTCGAACGGGATCTTCCACTCGATGCCGTTCTGGGACGAGCGCTTCGCCGCGATCGGCGCCGAATACCCCGAGA
TCCGCGCCGACCAGTACCATATCGACATCCTCGCGGCGCGCTTCGTGATGAGCCCGGAGCGGTTCGACGTGGTGGTCGGC
TCGAACCTGTTCGGGGACATCCTTTCAGACCTCGGCCCCGGCGTGACCGGCACGATCGCCGTCGCCCCCTCGGCCAATCT
CAACCCGGAGAAGAAGTTCCCCTCGATGTTCGAGCCGGTGCATGGCTCCGCCCCCGACATCTTCGGCCAGCGCATCGCCA
ATCCGGTGGGCGCGATCTGGGCCGGCGCGATGATGCTCGATCATCTCGGCGAGGCCGAGGCCGCGGCGCTGGTTCTCCGC
GCGATCGAGACCGCCCTCGGCGATCCCGCCTCGCCGCGCACGCCCGATCTCGGCGGCACCGCGAAGATGGACGATCTCGC
CGCGGCCATCACCGCCGCGGTCTGA

Upstream 100 bases:

>100_bases
GCGTGCTGATCCTCTCGATCATCTACGGCCTGTCGCTCGCGAAGCGCGATCCCGATCTCGTGCAGAAGATCGGCTCCTAC
GTCGCCGACGAGGAATACTG

Downstream 100 bases:

>100_bases
TCCCGCTCAGCGGAGCGGCTTGGCGAAGCCGAACAGGGTGATCGCGAGGCCCTCGCGGAAATAGAACCGGTGCGCCCGCT
CGCGGTGGGTGCCGGATTCG

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSRRHRIAVIEGDGIGREVIPAARTVLDRAASACGFTLDWRDFDWSCETWKATGRMMPEDGIARLADHDAIFLGAVGYPG
VPDHISLWGLLIPIRREFDQYVNLRPVRLLPGVPCPLANKQPGDIDFWVVRENVEGEYSQIGGRMFAGTEREFVEQTAIF
TRAGTDRILRYAFDLARRLGRPHVTSATKSNGIFHSMPFWDERFAAIGAEYPEIRADQYHIDILAARFVMSPERFDVVVG
SNLFGDILSDLGPGVTGTIAVAPSANLNPEKKFPSMFEPVHGSAPDIFGQRIANPVGAIWAGAMMLDHLGEAEAAALVLR
AIETALGDPASPRTPDLGGTAKMDDLAAAITAAV

Sequences:

>Translated_354_residues
MSRRHRIAVIEGDGIGREVIPAARTVLDRAASACGFTLDWRDFDWSCETWKATGRMMPEDGIARLADHDAIFLGAVGYPG
VPDHISLWGLLIPIRREFDQYVNLRPVRLLPGVPCPLANKQPGDIDFWVVRENVEGEYSQIGGRMFAGTEREFVEQTAIF
TRAGTDRILRYAFDLARRLGRPHVTSATKSNGIFHSMPFWDERFAAIGAEYPEIRADQYHIDILAARFVMSPERFDVVVG
SNLFGDILSDLGPGVTGTIAVAPSANLNPEKKFPSMFEPVHGSAPDIFGQRIANPVGAIWAGAMMLDHLGEAEAAALVLR
AIETALGDPASPRTPDLGGTAKMDDLAAAITAAV
>Mature_353_residues
SRRHRIAVIEGDGIGREVIPAARTVLDRAASACGFTLDWRDFDWSCETWKATGRMMPEDGIARLADHDAIFLGAVGYPGV
PDHISLWGLLIPIRREFDQYVNLRPVRLLPGVPCPLANKQPGDIDFWVVRENVEGEYSQIGGRMFAGTEREFVEQTAIFT
RAGTDRILRYAFDLARRLGRPHVTSATKSNGIFHSMPFWDERFAAIGAEYPEIRADQYHIDILAARFVMSPERFDVVVGS
NLFGDILSDLGPGVTGTIAVAPSANLNPEKKFPSMFEPVHGSAPDIFGQRIANPVGAIWAGAMMLDHLGEAEAAALVLRA
IETALGDPASPRTPDLGGTAKMDDLAAAITAAV

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=31.1428571428571, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4758582, Length=339, Percent_Identity=29.7935103244838, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI28178838, Length=328, Percent_Identity=28.9634146341463, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI28178816, Length=354, Percent_Identity=27.683615819209, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI28178821, Length=350, Percent_Identity=26.8571428571429, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI28178819, Length=212, Percent_Identity=29.7169811320755, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788101, Length=361, Percent_Identity=61.7728531855956, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI87081683, Length=368, Percent_Identity=36.4130434782609, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787381, Length=364, Percent_Identity=28.8461538461538, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71986051, Length=349, Percent_Identity=31.5186246418338, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=353, Percent_Identity=28.328611898017, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=26.271186440678, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=355, Percent_Identity=27.6056338028169, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=33.8068181818182, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=30.8988764044944, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=355, Percent_Identity=28.4507042253521, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6319830, Length=372, Percent_Identity=28.7634408602151, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24643270, Length=353, Percent_Identity=29.7450424929178, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24643268, Length=353, Percent_Identity=29.7450424929178, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24661184, Length=350, Percent_Identity=30.2857142857143, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI161078635, Length=351, Percent_Identity=29.3447293447293, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161078637, Length=351, Percent_Identity=29.3447293447293, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161078633, Length=351, Percent_Identity=29.3447293447293, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24650122, Length=351, Percent_Identity=29.3447293447293, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161078639, Length=261, Percent_Identity=32.5670498084291, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281362242, Length=353, Percent_Identity=28.0453257790368, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648872, Length=353, Percent_Identity=28.0453257790368, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI20130355, Length=347, Percent_Identity=24.7838616714697, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 38610; Mature: 38479

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRHRIAVIEGDGIGREVIPAARTVLDRAASACGFTLDWRDFDWSCETWKATGRMMPED
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCCCCCCH
GIARLADHDAIFLGAVGYPGVPDHISLWGLLIPIRREFDQYVNLRPVRLLPGVPCPLANK
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
QPGDIDFWVVRENVEGEYSQIGGRMFAGTEREFVEQTAIFTRAGTDRILRYAFDLARRLG
CCCCEEEEEEECCCCCHHHHHCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
RPHVTSATKSNGIFHSMPFWDERFAAIGAEYPEIRADQYHIDILAARFVMSPERFDVVVG
CCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEC
SNLFGDILSDLGPGVTGTIAVAPSANLNPEKKFPSMFEPVHGSAPDIFGQRIANPVGAIW
CHHHHHHHHHHCCCCEEEEEECCCCCCCCHHCCCHHHCCCCCCCCHHHHHHHCCHHHHHH
AGAMMLDHLGEAEAAALVLRAIETALGDPASPRTPDLGGTAKMDDLAAAITAAV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SRRHRIAVIEGDGIGREVIPAARTVLDRAASACGFTLDWRDFDWSCETWKATGRMMPED
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCCCCCCH
GIARLADHDAIFLGAVGYPGVPDHISLWGLLIPIRREFDQYVNLRPVRLLPGVPCPLANK
HHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
QPGDIDFWVVRENVEGEYSQIGGRMFAGTEREFVEQTAIFTRAGTDRILRYAFDLARRLG
CCCCEEEEEEECCCCCHHHHHCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
RPHVTSATKSNGIFHSMPFWDERFAAIGAEYPEIRADQYHIDILAARFVMSPERFDVVVG
CCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEC
SNLFGDILSDLGPGVTGTIAVAPSANLNPEKKFPSMFEPVHGSAPDIFGQRIANPVGAIW
CHHHHHHHHHHCCCCEEEEEECCCCCCCCHHCCCHHHCCCCCCCCHHHHHHHCCHHHHHH
AGAMMLDHLGEAEAAALVLRAIETALGDPASPRTPDLGGTAKMDDLAAAITAAV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]