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The map label for this gene is potI [H]

Identifier: 148259556

GI number: 148259556

Start: 602900

End: 603742

Strand: Reverse

Name: potI [H]

Synonym: Acry_0540

Alternate gene names: 148259556

Gene position: 603742-602900 (Counterclockwise)

Preceding gene: 148259557

Following gene: 148259555

Centisome position: 17.81

GC content: 64.06

Gene sequence:

>843_bases
ATGATGGCCATCTCGATGGGAAAATCGACGCGCCGTTCGCTGCTGGCGATGAGCCTGCCGGTCTATCTGCTGCTCTATGC
GCCACTCCTTCTGATCGCGATCTTTTCCTTCGCGCCGCCGCCGCTGGCGAAGGGACAGACCTTCTACTGGTATGCCGACC
TGCTGCATTCGCCCGAGGTCATCGCGGCGTTCCGGCGATCGCTGACGCTGGCGATCGGCTCCTCGATCCTGGGGACGGCT
CTGGGCACGCTGATGGGCTTCGGCCTCTATCGTTACCGCGACCGCAACGCGACCGGCATCGCCAGCGCGCTGCCGAGCCT
GATCATCTACACGCCGATCATCATGCCGAGCCTGGTGTTCGGGATTTCGGAGCTGATCTTCTTCAGCTTCATCCACAGCG
CGACCGGATTTCTGGCCGCCGGGCTGCTGACGATGGGCATCGCGCATGTCACCTTCGAGGCACCGTTCGTCGCCCTCGTG
GTGTTCGCGCGGCTGTCGGGGCTCGATCCGAACATGTTCGAGGCCTCGCACGATCTCTACGCCGATGCGCGGCAATCCTT
CCTGCACCTGACCGTGCCGGTGATCCGGCCGGCGATCATCGGCGGTTTCCTGCTCGCCTTCACGCTGTCGATCGACGATT
TCACGATCAGCTTCTTCACCGCCGGGCCGGGCAGCACGACGCTGCCGATCTACATCTACAGCGCGGTGGCGCGGAAGGGC
GTGTCGCCCGACATCAACGCGCTGGCCACGCTGATGATCGCCACCGTCATCGGCACCGGGCTGCTGCAATACATGTTCAC
GAGAAACCGGGCGCGAAAGCTCGTCGCGCGACCTGGAGACTGA

Upstream 100 bases:

>100_bases
ACGATCTTCATGCTGGTGTTCACCTGTGCGGGGCTGCTCGCCTTCCGCGTGCTGAGCAAGCGGCTCGGCGGCGCCGAAGA
GAGCATGATGTGAGGCGCGG

Downstream 100 bases:

>100_bases
GACGGACGAGCCGCACAGGCAACGGATGACGACGCGGCAACGCGGATGAATCAACGCTACAGGAGGTTTTTGATGAAGGG
ACTCATGGGGAAGATCGCGG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MMAISMGKSTRRSLLAMSLPVYLLLYAPLLLIAIFSFAPPPLAKGQTFYWYADLLHSPEVIAAFRRSLTLAIGSSILGTA
LGTLMGFGLYRYRDRNATGIASALPSLIIYTPIIMPSLVFGISELIFFSFIHSATGFLAAGLLTMGIAHVTFEAPFVALV
VFARLSGLDPNMFEASHDLYADARQSFLHLTVPVIRPAIIGGFLLAFTLSIDDFTISFFTAGPGSTTLPIYIYSAVARKG
VSPDINALATLMIATVIGTGLLQYMFTRNRARKLVARPGD

Sequences:

>Translated_280_residues
MMAISMGKSTRRSLLAMSLPVYLLLYAPLLLIAIFSFAPPPLAKGQTFYWYADLLHSPEVIAAFRRSLTLAIGSSILGTA
LGTLMGFGLYRYRDRNATGIASALPSLIIYTPIIMPSLVFGISELIFFSFIHSATGFLAAGLLTMGIAHVTFEAPFVALV
VFARLSGLDPNMFEASHDLYADARQSFLHLTVPVIRPAIIGGFLLAFTLSIDDFTISFFTAGPGSTTLPIYIYSAVARKG
VSPDINALATLMIATVIGTGLLQYMFTRNRARKLVARPGD
>Mature_280_residues
MMAISMGKSTRRSLLAMSLPVYLLLYAPLLLIAIFSFAPPPLAKGQTFYWYADLLHSPEVIAAFRRSLTLAIGSSILGTA
LGTLMGFGLYRYRDRNATGIASALPSLIIYTPIIMPSLVFGISELIFFSFIHSATGFLAAGLLTMGIAHVTFEAPFVALV
VFARLSGLDPNMFEASHDLYADARQSFLHLTVPVIRPAIIGGFLLAFTLSIDDFTISFFTAGPGSTTLPIYIYSAVARKG
VSPDINALATLMIATVIGTGLLQYMFTRNRARKLVARPGD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=259, Percent_Identity=39.7683397683398, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1787368, Length=229, Percent_Identity=33.6244541484716, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787714, Length=236, Percent_Identity=30.0847457627119, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788764, Length=183, Percent_Identity=30.6010928961749, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30271; Mature: 30271

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAISMGKSTRRSLLAMSLPVYLLLYAPLLLIAIFSFAPPPLAKGQTFYWYADLLHSPEV
CCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCHHH
IAAFRRSLTLAIGSSILGTALGTLMGFGLYRYRDRNATGIASALPSLIIYTPIIMPSLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GISELIFFSFIHSATGFLAAGLLTMGIAHVTFEAPFVALVVFARLSGLDPNMFEASHDLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ADARQSFLHLTVPVIRPAIIGGFLLAFTLSIDDFTISFFTAGPGSTTLPIYIYSAVARKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCHHHHHHHHHHHCC
VSPDINALATLMIATVIGTGLLQYMFTRNRARKLVARPGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMAISMGKSTRRSLLAMSLPVYLLLYAPLLLIAIFSFAPPPLAKGQTFYWYADLLHSPEV
CCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCHHH
IAAFRRSLTLAIGSSILGTALGTLMGFGLYRYRDRNATGIASALPSLIIYTPIIMPSLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GISELIFFSFIHSATGFLAAGLLTMGIAHVTFEAPFVALVVFARLSGLDPNMFEASHDLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ADARQSFLHLTVPVIRPAIIGGFLLAFTLSIDDFTISFFTAGPGSTTLPIYIYSAVARKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCHHHHHHHHHHHCC
VSPDINALATLMIATVIGTGLLQYMFTRNRARKLVARPGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]