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The map label for this gene is potB [H]

Identifier: 148259557

GI number: 148259557

Start: 603750

End: 604682

Strand: Reverse

Name: potB [H]

Synonym: Acry_0541

Alternate gene names: 148259557

Gene position: 604682-603750 (Counterclockwise)

Preceding gene: 148259558

Following gene: 148259556

Centisome position: 17.84

GC content: 66.35

Gene sequence:

>933_bases
ATGAGCGCAACGACGGCAGACGCACGGCGAGGCAGTGCCGCGCGGGCGGAGCGCCTCGGCCGCGCCTTGCGGCTTTGGGT
CACCGCCGGGCCGGGCCTCACCTGGATCGTGCTGTTCCTCGTATTGCCGAGCGCCATTTTGCTCGGCATCGGTTTCTTCA
CCTCGGGCAGCTATGGTGGACCCGAATTGCCGCTAACCCTGCAGCCGTTGCGCGAGGTTGCCGGCTATTCGATCCTGGGC
TGGAGTTCCGGCAACATCACGGTGCTCGTGCGGTCGCTCATCCAGGCAATGAGCGCCGGCGTGCTGACGGTGCTGTTCGC
CTATCCGGTCGTGTTCTACATCGTCACCCGCCCGCCGGCGGCGCGGCCACTTCTGCTGCTGTTCATCGTGCTGCCGTCCT
GGACCAACCAGGTGGTGCGGACCTATGCATGGATGGAGCTGCTCGGGCCGGAATCGGCGCTGTCGCATCTCGTGCAGTCG
CTCGGGCTGCTGCAGCCGCATCTCGGCCTGTTTCCGGGCGTGCTCGCGATGCAGATCGGGCTGACCTACAATTATTTGCC
CTACATGGTCGTGCCGCTCTACGCCGCGGCGGAGAAGCTCGACTGGGCGCTGGTCGAGGCCGGGCGGGATCTCTACGCCT
CCGGCCCCAGGCTGTTCTGGAACACGGTGTTTCCGCAGACGCTGCCGGGGCTGCTCTCGGGCATCATCCTCGTCGTCATC
CCGGCCTTCGGCGATTACGTGGTGCCGACGCTGCTCGGCGGCGGCAAGTCGATGATGATCGGCTCGCTGATCGCCTCGCA
GTTCCTGGAAACGCCGAACTGGCCCTACGGGGCGGCGCTGACGATCTTCATGCTGGTGTTCACCTGTGCGGGGCTGCTCG
CCTTCCGCGTGCTGAGCAAGCGGCTCGGCGGCGCCGAAGAGAGCATGATGTGA

Upstream 100 bases:

>100_bases
TCGTCTACGACACCAACAACCAGGCGGCCGAACGCAGCTGGCGGCCCGGCGAGGCGGTGACCTGCGAGCTGAAGCCCGAA
AATATCGTGCTCATCGGCTC

Downstream 100 bases:

>100_bases
GGCGCGGATGATGGCCATCTCGATGGGAAAATCGACGCGCCGTTCGCTGCTGGCGATGAGCCTGCCGGTCTATCTGCTGC
TCTATGCGCCACTCCTTCTG

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSATTADARRGSAARAERLGRALRLWVTAGPGLTWIVLFLVLPSAILLGIGFFTSGSYGGPELPLTLQPLREVAGYSILG
WSSGNITVLVRSLIQAMSAGVLTVLFAYPVVFYIVTRPPAARPLLLLFIVLPSWTNQVVRTYAWMELLGPESALSHLVQS
LGLLQPHLGLFPGVLAMQIGLTYNYLPYMVVPLYAAAEKLDWALVEAGRDLYASGPRLFWNTVFPQTLPGLLSGIILVVI
PAFGDYVVPTLLGGGKSMMIGSLIASQFLETPNWPYGAALTIFMLVFTCAGLLAFRVLSKRLGGAEESMM

Sequences:

>Translated_310_residues
MSATTADARRGSAARAERLGRALRLWVTAGPGLTWIVLFLVLPSAILLGIGFFTSGSYGGPELPLTLQPLREVAGYSILG
WSSGNITVLVRSLIQAMSAGVLTVLFAYPVVFYIVTRPPAARPLLLLFIVLPSWTNQVVRTYAWMELLGPESALSHLVQS
LGLLQPHLGLFPGVLAMQIGLTYNYLPYMVVPLYAAAEKLDWALVEAGRDLYASGPRLFWNTVFPQTLPGLLSGIILVVI
PAFGDYVVPTLLGGGKSMMIGSLIASQFLETPNWPYGAALTIFMLVFTCAGLLAFRVLSKRLGGAEESMM
>Mature_309_residues
SATTADARRGSAARAERLGRALRLWVTAGPGLTWIVLFLVLPSAILLGIGFFTSGSYGGPELPLTLQPLREVAGYSILGW
SSGNITVLVRSLIQAMSAGVLTVLFAYPVVFYIVTRPPAARPLLLLFIVLPSWTNQVVRTYAWMELLGPESALSHLVQSL
GLLQPHLGLFPGVLAMQIGLTYNYLPYMVVPLYAAAEKLDWALVEAGRDLYASGPRLFWNTVFPQTLPGLLSGIILVVIP
AFGDYVVPTLLGGGKSMMIGSLIASQFLETPNWPYGAALTIFMLVFTCAGLLAFRVLSKRLGGAEESMM

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=271, Percent_Identity=34.6863468634686, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787080, Length=217, Percent_Identity=35.0230414746544, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1787713, Length=274, Percent_Identity=29.1970802919708, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33433; Mature: 33302

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATTADARRGSAARAERLGRALRLWVTAGPGLTWIVLFLVLPSAILLGIGFFTSGSYGG
CCCCCCCHHCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PELPLTLQPLREVAGYSILGWSSGNITVLVRSLIQAMSAGVLTVLFAYPVVFYIVTRPPA
CCCCEEHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ARPLLLLFIVLPSWTNQVVRTYAWMELLGPESALSHLVQSLGLLQPHLGLFPGVLAMQIG
CCHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
LTYNYLPYMVVPLYAAAEKLDWALVEAGRDLYASGPRLFWNTVFPQTLPGLLSGIILVVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHH
PAFGDYVVPTLLGGGKSMMIGSLIASQFLETPNWPYGAALTIFMLVFTCAGLLAFRVLSK
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLGGAEESMM
HHCCCHHCCC
>Mature Secondary Structure 
SATTADARRGSAARAERLGRALRLWVTAGPGLTWIVLFLVLPSAILLGIGFFTSGSYGG
CCCCCCHHCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC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HHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]