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The map label for this gene is rfbD [H]

Identifier: 148259523

GI number: 148259523

Start: 564596

End: 565492

Strand: Reverse

Name: rfbD [H]

Synonym: Acry_0507

Alternate gene names: 148259523

Gene position: 565492-564596 (Counterclockwise)

Preceding gene: 148259524

Following gene: 148259518

Centisome position: 16.68

GC content: 65.89

Gene sequence:

>897_bases
ATGGGACAATTGAGACGGATACTTGTCACCGGCGCCGGCGGCTTCGTCGGGCGGCACCTGATGCCTGAACTTGGCCGCCG
ATTTCCGTGGGCCGAACTGGTCAGCTTCCGCGCCGATATCACCGACCCCGCCGCCACCGAGACCTCGGTGCGCGACGTCC
GGCCGGATGCCTGCGTCCATCTCGCGGGCATCGCCGCCATTCCCGAGGCACGGGAACACCCACGCCGTGCCTTCGCGGTG
AACCTCGACGGCACGCTGAATCTTGCTCGCGCTCTGCTTGCGCACGCACCTGGCTGCCAACTGATTCATGCCGGTAGTGC
CGATTGCTACGGAGCCAGTTTCCGCTCGGGCCAGGCGCTGGACGAATCGGCGCCTCTGGCGCCGCTCAACACCTATGCGG
CAAGCAAGGCCGCAGCGGATCTTACCCTTGGTGCCATGGCTGCCGAATCCGGACTGCGCGTGCTGCGATTCCGTCCGTTC
AACCATGTGGGCCCCGGGCAGAGCGAAGCCTTCGCCCTTGCCTCCTTTGCTGCCCAACTCCGCCGGATTGCCCGCGGCGA
TGCAGCACCGGTGCTGCATGTCGGCAACCTCGATGCCGAGCGCGACTTTCTTCACGTGAACGACGTGGTCCGTGCGTATG
CGCTGGCGCTGGGACGCTCCGACGACCTGCCGAACGGCACGATCTTCAACATTGCCTCCGGCATTCCGCGCCGGATGCGC
GACATGGTCGAGGCGATGATCGTCTCTCTCGGTATCCATGTCTCGGTCGAGATCGATCCGACCCGCCTTCGGCCAAGCGA
TATTCCGCGCGCGGTCGGCGATGCTTCCCGCGCCGAAAACCTGCTTGGCTGGCACCCGGACCACGGGATCGAGGAGATCG
TGCGCTCGGTCCTCTGA

Upstream 100 bases:

>100_bases
GATCCATTTGATCGGATGATGCTCTGATGCTCAATATGATAGAGCACTTTCCGATCCTGCAGGATCGGAAAGTGCTCTAG
ACGATGGAGACGTCCGGCCC

Downstream 100 bases:

>100_bases
GCGCCGCATCTGCGCCCCGAGGCGAAACCAGCCCTGAAGACACGACCGGCAGCCCGGCCGGAAACCGGCGGCAGATTCCG
CCGATGCGCCAGACTGCCGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MGQLRRILVTGAGGFVGRHLMPELGRRFPWAELVSFRADITDPAATETSVRDVRPDACVHLAGIAAIPEAREHPRRAFAV
NLDGTLNLARALLAHAPGCQLIHAGSADCYGASFRSGQALDESAPLAPLNTYAASKAAADLTLGAMAAESGLRVLRFRPF
NHVGPGQSEAFALASFAAQLRRIARGDAAPVLHVGNLDAERDFLHVNDVVRAYALALGRSDDLPNGTIFNIASGIPRRMR
DMVEAMIVSLGIHVSVEIDPTRLRPSDIPRAVGDASRAENLLGWHPDHGIEEIVRSVL

Sequences:

>Translated_298_residues
MGQLRRILVTGAGGFVGRHLMPELGRRFPWAELVSFRADITDPAATETSVRDVRPDACVHLAGIAAIPEAREHPRRAFAV
NLDGTLNLARALLAHAPGCQLIHAGSADCYGASFRSGQALDESAPLAPLNTYAASKAAADLTLGAMAAESGLRVLRFRPF
NHVGPGQSEAFALASFAAQLRRIARGDAAPVLHVGNLDAERDFLHVNDVVRAYALALGRSDDLPNGTIFNIASGIPRRMR
DMVEAMIVSLGIHVSVEIDPTRLRPSDIPRAVGDASRAENLLGWHPDHGIEEIVRSVL
>Mature_297_residues
GQLRRILVTGAGGFVGRHLMPELGRRFPWAELVSFRADITDPAATETSVRDVRPDACVHLAGIAAIPEAREHPRRAFAVN
LDGTLNLARALLAHAPGCQLIHAGSADCYGASFRSGQALDESAPLAPLNTYAASKAAADLTLGAMAAESGLRVLRFRPFN
HVGPGQSEAFALASFAAQLRRIARGDAAPVLHVGNLDAERDFLHVNDVVRAYALALGRSDDLPNGTIFNIASGIPRRMRD
MVEAMIVSLGIHVSVEIDPTRLRPSDIPRAVGDASRAENLLGWHPDHGIEEIVRSVL

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1788366, Length=291, Percent_Identity=24.7422680412371, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786974, Length=336, Percent_Identity=24.702380952381, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994969, Length=338, Percent_Identity=26.3313609467456, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71982035, Length=347, Percent_Identity=25.3602305475504, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=349, Percent_Identity=25.214899713467, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI133901786, Length=280, Percent_Identity=22.5, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=280, Percent_Identity=22.5, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=280, Percent_Identity=22.5, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=280, Percent_Identity=22.5, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=280, Percent_Identity=22.5, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=280, Percent_Identity=22.5, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24158427, Length=281, Percent_Identity=23.4875444839858, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 31886; Mature: 31755

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQLRRILVTGAGGFVGRHLMPELGRRFPWAELVSFRADITDPAATETSVRDVRPDACVH
CCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHH
LAGIAAIPEAREHPRRAFAVNLDGTLNLARALLAHAPGCQLIHAGSADCYGASFRSGQAL
HHHHHHCCCHHHCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC
DESAPLAPLNTYAASKAAADLTLGAMAAESGLRVLRFRPFNHVGPGQSEAFALASFAAQL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
RRIARGDAAPVLHVGNLDAERDFLHVNDVVRAYALALGRSDDLPNGTIFNIASGIPRRMR
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHCCHHHHH
DMVEAMIVSLGIHVSVEIDPTRLRPSDIPRAVGDASRAENLLGWHPDHGIEEIVRSVL
HHHHHHHHHHCCEEEEEECCCCCCHHHCCHHHCCHHHHHHHCCCCCCCCHHHHHHHHC
>Mature Secondary Structure 
GQLRRILVTGAGGFVGRHLMPELGRRFPWAELVSFRADITDPAATETSVRDVRPDACVH
CCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHH
LAGIAAIPEAREHPRRAFAVNLDGTLNLARALLAHAPGCQLIHAGSADCYGASFRSGQAL
HHHHHHCCCHHHCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC
DESAPLAPLNTYAASKAAADLTLGAMAAESGLRVLRFRPFNHVGPGQSEAFALASFAAQL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
RRIARGDAAPVLHVGNLDAERDFLHVNDVVRAYALALGRSDDLPNGTIFNIASGIPRRMR
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHCCHHHHH
DMVEAMIVSLGIHVSVEIDPTRLRPSDIPRAVGDASRAENLLGWHPDHGIEEIVRSVL
HHHHHHHHHHCCEEEEEECCCCCCHHHCCHHHCCHHHHHHHCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098074; 10521656 [H]