Click here to switch to the map view.

The map label for this gene is gca [H]

Identifier: 148259524

GI number: 148259524

Start: 565706

End: 566689

Strand: Reverse

Name: gca [H]

Synonym: Acry_0508

Alternate gene names: 148259524

Gene position: 566689-565706 (Counterclockwise)

Preceding gene: 148259525

Following gene: 148259523

Centisome position: 16.72

GC content: 64.13

Gene sequence:

>984_bases
ATGAAAACAGCACTCGTCACCGGGATCACCGGCCAGGACGGCGCCTATCTCTCCCAGTTGCTCCTGGGCAAGGGATACGA
GGTATTCGGCGTGATACGGCGCTCTTCGCATCGCGGCGTCGAAGACCATCGCCTGCGCTGGCTCGGCATCGCCGACAAGG
TCACCCTGCTTGACGGCGATCTCGCTGATCTTTCCAGCCTCGTGCGCATCGTCAAGGACGTCCGGCCCGACGAGGTCTAT
AATCTTGCCGCCCAGTCGTTCGTCGCCTCGTCCTGGCGGCAGCCGATTCTGACCGCCAACATCACCGCCGTCGGCGTCAC
CAACATCCTCGAGGCAATCCGCGCCGAACAGCCGGAGGCCCGCTTCTACCAGGCATCCTCCTCGGAAATGTATGGCCTGA
TCCAGGAGCCGATGCAGAGCGAGGCGACCCCCTTTTATCCCCGCTCGCCCTACGCCGTTGCTAAACTCTACGGCCACTGG
ATCACGGTGAACTACCGCGAGAGCTTCGGCATGCATGCCTCTTCGGGCATCCTGTTCAACCACGAAAGCCCGCTTCGCGG
CGTCGAGTTCGTCACCCGCAAGGTGACCGACGGCGTCGCCCGCATCAAGCTCGGCCTCGCCGGCGAACTGCGTCTTGGCA
ATGTCGACGCAAAGCGCGACTGGGGCCACGCACGGGATTACGTGAAGGCGATGTGGCTGATGCTCCAGCAGGACAAGCCG
GATGACTACGTCGTGGCCACCGGCCGCACTACCACGGTGCGGGACATGTGTCGTATCGCCTTCGACCATGCCGGCCTCGA
CATGGAGCGCCACCTCGTCATCGATCCCGCCTTCTACCGCCCCGCCGAGGTCGACGTGCTGCTGGGCAATCCCGCGAAGG
CGGAGCAGAAACTCGGCTGGCGGCCGGAAACCACGCTCGAGCAGATGATCATCGAAATGGTCGACGCCGATCTCGAACGC
CTGCGCCGCACCACGCCGGGCTGA

Upstream 100 bases:

>100_bases
GGCGGGCTCGCAATCGATGTAGTGGTCGCGCCTCCGGCCATTAATTTGACATTTTGGCGCATATAGATCCCGCCAAGTTT
ATTGGCTAAGGGATCGAAGC

Downstream 100 bases:

>100_bases
CCTCGCAGATTGCGGCCGCCATGCGAACGTTCCGCGCCGGCGGCGGACTGGCCACCGTCTCCAACTTCCACTAGCGTGCG
CCGCCCACAACTAGAGCATC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKTALVTGITGQDGAYLSQLLLGKGYEVFGVIRRSSHRGVEDHRLRWLGIADKVTLLDGDLADLSSLVRIVKDVRPDEVY
NLAAQSFVASSWRQPILTANITAVGVTNILEAIRAEQPEARFYQASSSEMYGLIQEPMQSEATPFYPRSPYAVAKLYGHW
ITVNYRESFGMHASSGILFNHESPLRGVEFVTRKVTDGVARIKLGLAGELRLGNVDAKRDWGHARDYVKAMWLMLQQDKP
DDYVVATGRTTTVRDMCRIAFDHAGLDMERHLVIDPAFYRPAEVDVLLGNPAKAEQKLGWRPETTLEQMIIEMVDADLER
LRRTTPG

Sequences:

>Translated_327_residues
MKTALVTGITGQDGAYLSQLLLGKGYEVFGVIRRSSHRGVEDHRLRWLGIADKVTLLDGDLADLSSLVRIVKDVRPDEVY
NLAAQSFVASSWRQPILTANITAVGVTNILEAIRAEQPEARFYQASSSEMYGLIQEPMQSEATPFYPRSPYAVAKLYGHW
ITVNYRESFGMHASSGILFNHESPLRGVEFVTRKVTDGVARIKLGLAGELRLGNVDAKRDWGHARDYVKAMWLMLQQDKP
DDYVVATGRTTTVRDMCRIAFDHAGLDMERHLVIDPAFYRPAEVDVLLGNPAKAEQKLGWRPETTLEQMIIEMVDADLER
LRRTTPG
>Mature_327_residues
MKTALVTGITGQDGAYLSQLLLGKGYEVFGVIRRSSHRGVEDHRLRWLGIADKVTLLDGDLADLSSLVRIVKDVRPDEVY
NLAAQSFVASSWRQPILTANITAVGVTNILEAIRAEQPEARFYQASSSEMYGLIQEPMQSEATPFYPRSPYAVAKLYGHW
ITVNYRESFGMHASSGILFNHESPLRGVEFVTRKVTDGVARIKLGLAGELRLGNVDAKRDWGHARDYVKAMWLMLQQDKP
DDYVVATGRTTTVRDMCRIAFDHAGLDMERHLVIDPAFYRPAEVDVLLGNPAKAEQKLGWRPETTLEQMIIEMVDADLER
LRRTTPG

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=346, Percent_Identity=48.2658959537572, Blast_Score=329, Evalue=2e-90,
Organism=Escherichia coli, GI1788366, Length=354, Percent_Identity=51.4124293785311, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI48994969, Length=329, Percent_Identity=23.1003039513678, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788353, Length=336, Percent_Identity=21.7261904761905, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=342, Percent_Identity=49.7076023391813, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17539424, Length=343, Percent_Identity=49.8542274052478, Blast_Score=333, Evalue=8e-92,
Organism=Caenorhabditis elegans, GI133901790, Length=343, Percent_Identity=49.8542274052478, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI133901788, Length=343, Percent_Identity=49.8542274052478, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17539422, Length=343, Percent_Identity=49.8542274052478, Blast_Score=332, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI133901786, Length=343, Percent_Identity=49.8542274052478, Blast_Score=332, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24158427, Length=349, Percent_Identity=48.4240687679083, Blast_Score=326, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 36677; Mature: 36677

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALVTGITGQDGAYLSQLLLGKGYEVFGVIRRSSHRGVEDHRLRWLGIADKVTLLDGD
CCCEEEECCCCCCHHHHHHHHHCCCCCEEHEEECCCCCCCHHHCEEEEECCCEEEEECCC
LADLSSLVRIVKDVRPDEVYNLAAQSFVASSWRQPILTANITAVGVTNILEAIRAEQPEA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCHH
RFYQASSSEMYGLIQEPMQSEATPFYPRSPYAVAKLYGHWITVNYRESFGMHASSGILFN
HHEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEEHHHCCCCCCCCEEEC
HESPLRGVEFVTRKVTDGVARIKLGLAGELRLGNVDAKRDWGHARDYVKAMWLMLQQDKP
CCCCCHHHHHHHHHHHCCHHEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCC
DDYVVATGRTTTVRDMCRIAFDHAGLDMERHLVIDPAFYRPAEVDVLLGNPAKAEQKLGW
CCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHCCC
RPETTLEQMIIEMVDADLERLRRTTPG
CCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTALVTGITGQDGAYLSQLLLGKGYEVFGVIRRSSHRGVEDHRLRWLGIADKVTLLDGD
CCCEEEECCCCCCHHHHHHHHHCCCCCEEHEEECCCCCCCHHHCEEEEECCCEEEEECCC
LADLSSLVRIVKDVRPDEVYNLAAQSFVASSWRQPILTANITAVGVTNILEAIRAEQPEA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCHH
RFYQASSSEMYGLIQEPMQSEATPFYPRSPYAVAKLYGHWITVNYRESFGMHASSGILFN
HHEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEEHHHCCCCCCCCEEEC
HESPLRGVEFVTRKVTDGVARIKLGLAGELRLGNVDAKRDWGHARDYVKAMWLMLQQDKP
CCCCCHHHHHHHHHHHCCHHEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCC
DDYVVATGRTTTVRDMCRIAFDHAGLDMERHLVIDPAFYRPAEVDVLLGNPAKAEQKLGW
CCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHCCC
RPETTLEQMIIEMVDADLERLRRTTPG
CCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]