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The map label for this gene is paaI [H]

Identifier: 148259494

GI number: 148259494

Start: 531631

End: 532092

Strand: Reverse

Name: paaI [H]

Synonym: Acry_0478

Alternate gene names: 148259494

Gene position: 532092-531631 (Counterclockwise)

Preceding gene: 148259495

Following gene: 148259493

Centisome position: 15.7

GC content: 68.4

Gene sequence:

>462_bases
ATGACGGAGGAACATCAGCCGCCCCCACCCGAGTCCGCGGACAACATTGCCCGCCATGTCGGCCAGGCGATGTTCGAGGT
GGACCGGGCGACGCGGTCGCTCGGCATCGAGCTCGACGAGATCGGGCCCGGTTTCGCGACACTCTCGCTGCGTCTCGGCG
CGGCAATGGCCAACGGGCACGGCACCGCGCATGGCGGGCTGATCTTCACCCTGGCCGATTCGGCCTTCGCCTTCGCCTGC
AACTCACATGGCGAGCGGGCGGTGGCGGCGCATTGCTCGATCACCTTCCTTGCACCGGCGCGGCCGGGGGAGCGGCTCAT
CGCCACCGCGCGGGAGGTGGTACGGCGCGGGCGGAACGGAATCTACGATGTTTCGGTCAGCAACGATGACGGAATCGTCG
CGGCGTTCCGCGGACAGTCGCGCACGATCGGCGGCTCGTGGCTCGATGACAACGGAAGGTAG

Upstream 100 bases:

>100_bases
CGCGGCTGGCGCAGCAGCGGTGGTTGCGCGATCCGGCTTTTGCGGCACACTCGGGGCAAGCGCCGTGCAACGGCGCGTCC
GAGGAGGAACGCACGCCGCC

Downstream 100 bases:

>100_bases
CGGGATGGAGCGGGACGTCGAATTCGCCTCGCGTGACGAGGTTGTCGCGCTGCAGACGAGGCGGCTGAGGGAGGCGCTGC
GCCATGCCTATGAGCGCGTG

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MTEEHQPPPPESADNIARHVGQAMFEVDRATRSLGIELDEIGPGFATLSLRLGAAMANGHGTAHGGLIFTLADSAFAFAC
NSHGERAVAAHCSITFLAPARPGERLIATAREVVRRGRNGIYDVSVSNDDGIVAAFRGQSRTIGGSWLDDNGR

Sequences:

>Translated_153_residues
MTEEHQPPPPESADNIARHVGQAMFEVDRATRSLGIELDEIGPGFATLSLRLGAAMANGHGTAHGGLIFTLADSAFAFAC
NSHGERAVAAHCSITFLAPARPGERLIATAREVVRRGRNGIYDVSVSNDDGIVAAFRGQSRTIGGSWLDDNGR
>Mature_152_residues
TEEHQPPPPESADNIARHVGQAMFEVDRATRSLGIELDEIGPGFATLSLRLGAAMANGHGTAHGGLIFTLADSAFAFACN
SHGERAVAAHCSITFLAPARPGERLIATAREVVRRGRNGIYDVSVSNDDGIVAAFRGQSRTIGGSWLDDNGR

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=125, Percent_Identity=44.8, Blast_Score=113, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 16167; Mature: 16036

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEHQPPPPESADNIARHVGQAMFEVDRATRSLGIELDEIGPGFATLSLRLGAAMANGH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEECEEEECCC
GTAHGGLIFTLADSAFAFACNSHGERAVAAHCSITFLAPARPGERLIATAREVVRRGRNG
CCCCCCEEEEECCCEEEEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCC
IYDVSVSNDDGIVAAFRGQSRTIGGSWLDDNGR
EEEEEECCCCCEEEEECCCCEECCCCCCCCCCC
>Mature Secondary Structure 
TEEHQPPPPESADNIARHVGQAMFEVDRATRSLGIELDEIGPGFATLSLRLGAAMANGH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEEECEEEECCC
GTAHGGLIFTLADSAFAFACNSHGERAVAAHCSITFLAPARPGERLIATAREVVRRGRNG
CCCCCCEEEEECCCEEEEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCC
IYDVSVSNDDGIVAAFRGQSRTIGGSWLDDNGR
EEEEEECCCCCEEEEECCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]