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The map label for this gene is livM [H]

Identifier: 148259375

GI number: 148259375

Start: 406376

End: 407302

Strand: Reverse

Name: livM [H]

Synonym: Acry_0358

Alternate gene names: 148259375

Gene position: 407302-406376 (Counterclockwise)

Preceding gene: 148259376

Following gene: 148259374

Centisome position: 12.02

GC content: 66.77

Gene sequence:

>927_bases
ATGAGCGCATCCGCAAGCCGCAACACCGGCATCGCCGTCTGGATCCTCGGCCTGTTTCTGCCGGTCGTGCTGAACAGCGC
CTGGGTCTCGGTTGCCGCGATCTTCGCGATCTATGGCATCGTCGCCCTGTCGGAAGACATCATTCTCGGCCGCGCCGGCA
TGTTCGACATGGGGCACGCCGTCTATTTCGGCATCGGCGCCTATGTCACGGCGATCCTGAACGTCACCTTCGACTGGCCG
ATCTTCCTGACCATCCCCTTCGCCATCCTGCTGGCCGCCGCCTTCGGCGCCCTGCTCGCCGCCCCGCTGATCCGCCTGCG
CGGCGACTACCTGCTGGTCGCCAGCATCGGCTTCAACGCGATCTTCGTGCTGGCGATGCAGAACAACCTGTTCGGCCTCA
CCGGCGGGTCGGACGGCATCACCGGCATCGGCGTGCCCTCGCTGTTCGGCCTGCCGATCGAGAGCCAGAGCGCGATGTAT
TGGCTCGACTGGGTGGTGCTCGGCATCGTGCTGGTGCTGATGCGCAATCTCGACGGCTCGGAATTCGGCCGCGCGCTGCG
CTATCTCAAGCAGGACGAACTCGCCGCGACGACGATCGGCGTCAATCCGCGCAAGGTGAAGACGCTCGCCTTCGCCATCG
GCGCCGGGCTCGCCGGGCTCGCCGGCACGCTGTTCGCGGTGCAGCTCTCCACGGTCAGCCCGTCCTCCTTCAAGTTCACC
GAATCGGTGACGCTGTTCGCCATCGTCATCGTCGGCGGCCAGGGCTCGATTCCCGGCGTGCTGCTCGGCACCGCGCTGAT
GTTCGTGCTGCCCCAGGTCTTCCGCGACTTCGCGCAATACCGCTACCTGGTCTTCGGCTTCGCGATGATCCTGGTGATGG
TGCTGCGCCCGCAGGGCCTGTGGCCGCGGCGGGGGGCGCGGGCATGA

Upstream 100 bases:

>100_bases
CCAGCTCCTGGCAGGATGCGATCACCTTCGCGCTGCTGATCGGGATCCTGCTGGTGCGGCCTTCTGGACTCCTGGGCGAA
CGCGTCGCGGAGAAGGTGTG

Downstream 100 bases:

>100_bases
GCGCGCTGCTCGAACTCCACGGGGTCGGCATCCGCTTCGGCGGGCTGCGCGCGGTCGACAGGCTCTCCTTCACCGTCGAC
AAGGGGCAGATCGTCGCCCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSASASRNTGIAVWILGLFLPVVLNSAWVSVAAIFAIYGIVALSEDIILGRAGMFDMGHAVYFGIGAYVTAILNVTFDWP
IFLTIPFAILLAAAFGALLAAPLIRLRGDYLLVASIGFNAIFVLAMQNNLFGLTGGSDGITGIGVPSLFGLPIESQSAMY
WLDWVVLGIVLVLMRNLDGSEFGRALRYLKQDELAATTIGVNPRKVKTLAFAIGAGLAGLAGTLFAVQLSTVSPSSFKFT
ESVTLFAIVIVGGQGSIPGVLLGTALMFVLPQVFRDFAQYRYLVFGFAMILVMVLRPQGLWPRRGARA

Sequences:

>Translated_308_residues
MSASASRNTGIAVWILGLFLPVVLNSAWVSVAAIFAIYGIVALSEDIILGRAGMFDMGHAVYFGIGAYVTAILNVTFDWP
IFLTIPFAILLAAAFGALLAAPLIRLRGDYLLVASIGFNAIFVLAMQNNLFGLTGGSDGITGIGVPSLFGLPIESQSAMY
WLDWVVLGIVLVLMRNLDGSEFGRALRYLKQDELAATTIGVNPRKVKTLAFAIGAGLAGLAGTLFAVQLSTVSPSSFKFT
ESVTLFAIVIVGGQGSIPGVLLGTALMFVLPQVFRDFAQYRYLVFGFAMILVMVLRPQGLWPRRGARA
>Mature_307_residues
SASASRNTGIAVWILGLFLPVVLNSAWVSVAAIFAIYGIVALSEDIILGRAGMFDMGHAVYFGIGAYVTAILNVTFDWPI
FLTIPFAILLAAAFGALLAAPLIRLRGDYLLVASIGFNAIFVLAMQNNLFGLTGGSDGITGIGVPSLFGLPIESQSAMYW
LDWVVLGIVLVLMRNLDGSEFGRALRYLKQDELAATTIGVNPRKVKTLAFAIGAGLAGLAGTLFAVQLSTVSPSSFKFTE
SVTLFAIVIVGGQGSIPGVLLGTALMFVLPQVFRDFAQYRYLVFGFAMILVMVLRPQGLWPRRGARA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=305, Percent_Identity=36.3934426229508, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 32821; Mature: 32690

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASASRNTGIAVWILGLFLPVVLNSAWVSVAAIFAIYGIVALSEDIILGRAGMFDMGHA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHH
VYFGIGAYVTAILNVTFDWPIFLTIPFAILLAAAFGALLAAPLIRLRGDYLLVASIGFNA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCE
IFVLAMQNNLFGLTGGSDGITGIGVPSLFGLPIESQSAMYWLDWVVLGIVLVLMRNLDGS
EEEEEEECCEEEECCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
EFGRALRYLKQDELAATTIGVNPRKVKTLAFAIGAGLAGLAGTLFAVQLSTVSPSSFKFT
HHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCCCEEH
ESVTLFAIVIVGGQGSIPGVLLGTALMFVLPQVFRDFAQYRYLVFGFAMILVMVLRPQGL
HCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WPRRGARA
CCCCCCCC
>Mature Secondary Structure 
SASASRNTGIAVWILGLFLPVVLNSAWVSVAAIFAIYGIVALSEDIILGRAGMFDMGHA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHH
VYFGIGAYVTAILNVTFDWPIFLTIPFAILLAAAFGALLAAPLIRLRGDYLLVASIGFNA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCE
IFVLAMQNNLFGLTGGSDGITGIGVPSLFGLPIESQSAMYWLDWVVLGIVLVLMRNLDGS
EEEEEEECCEEEECCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
EFGRALRYLKQDELAATTIGVNPRKVKTLAFAIGAGLAGLAGTLFAVQLSTVSPSSFKFT
HHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCCCEEH
ESVTLFAIVIVGGQGSIPGVLLGTALMFVLPQVFRDFAQYRYLVFGFAMILVMVLRPQGL
HCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WPRRGARA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]