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The map label for this gene is hupD [H]

Identifier: 148259354

GI number: 148259354

Start: 384430

End: 385005

Strand: Reverse

Name: hupD [H]

Synonym: Acry_0335

Alternate gene names: 148259354

Gene position: 385005-384430 (Counterclockwise)

Preceding gene: 148259355

Following gene: 148259353

Centisome position: 11.36

GC content: 74.31

Gene sequence:

>576_bases
ATGAGCCGCGGGGCGACGGTGCTCGGGATCGGTAACGTGCTCTGGGCGGACGAGGGGTTCGGCGTGCGCGCGGTGGAGGC
GCTGAATGCCGGCTGGACGTGCGGGCCGGAGGTGCGCCTCGTCGATGGCGGAACGCAGGGGCTGGCGCTGCTGCCGGTGG
TGGGCGAGGCCGGGCTTCTGGTCATTCTCGACGCGGTGGATTTCGGGCTGGCGCCGGGGACGCTGATGGTGGCGGCCGAC
GACGATGTGCCGCGCTACCTGACCGCAAAGAAGCTGAGCCTGCACCAGACGAGTTTCCAGGACGTGCTGGCGCTGGCGCA
GATTTCGGGGCGGGCGCCGGCGCGGATGGTGCTGGTCGGCGTGCAGCCCTGCGACCTCGACACCTATGGCGGCGGGCTGT
CGGCGCCGGTGGCGGCGCGGATCGGCGAGGCGGTGGACCTCGTGCGGGCGCATCTGGCCGGGGCGGGGTTCGCGCTCGCG
CCGCGCGCGGCCGGCGAGGAGGCGGAGCCGCTCGGCCCGGCCGCGGTGGCGATGACGTTCGGCGGACCGGCGGCGCAGAT
AGCGGAGGCATCGTGA

Upstream 100 bases:

>100_bases
TGGTCCATGTCTACATGTCGGTGCGGGAGGACATCATGTCGCGGCAGACGATGATCAGCACGATGATCAATGGCTGGCGC
TTCTTCAAGGACGACCGGGA

Downstream 100 bases:

>100_bases
TGGCGGGATCGGCGGCACTGGCGCGGCTCGCGGCGGCGGAGGGCGTGCGGGAGCTGGGCGGCGACGGGCTGGAGGCGGCG
CTGGCCGAGGGCACGACGCT

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MSRGATVLGIGNVLWADEGFGVRAVEALNAGWTCGPEVRLVDGGTQGLALLPVVGEAGLLVILDAVDFGLAPGTLMVAAD
DDVPRYLTAKKLSLHQTSFQDVLALAQISGRAPARMVLVGVQPCDLDTYGGGLSAPVAARIGEAVDLVRAHLAGAGFALA
PRAAGEEAEPLGPAAVAMTFGGPAAQIAEAS

Sequences:

>Translated_191_residues
MSRGATVLGIGNVLWADEGFGVRAVEALNAGWTCGPEVRLVDGGTQGLALLPVVGEAGLLVILDAVDFGLAPGTLMVAAD
DDVPRYLTAKKLSLHQTSFQDVLALAQISGRAPARMVLVGVQPCDLDTYGGGLSAPVAARIGEAVDLVRAHLAGAGFALA
PRAAGEEAEPLGPAAVAMTFGGPAAQIAEAS
>Mature_190_residues
SRGATVLGIGNVLWADEGFGVRAVEALNAGWTCGPEVRLVDGGTQGLALLPVVGEAGLLVILDAVDFGLAPGTLMVAADD
DVPRYLTAKKLSLHQTSFQDVLALAQISGRAPARMVLVGVQPCDLDTYGGGLSAPVAARIGEAVDLVRAHLAGAGFALAP
RAAGEEAEPLGPAAVAMTFGGPAAQIAEAS

Specific function: Not known. Could be involved in the processing of hydrogenase [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=129, Percent_Identity=58.9147286821705, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1789367, Length=159, Percent_Identity=40.8805031446541, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 19180; Mature: 19049

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRGATVLGIGNVLWADEGFGVRAVEALNAGWTCGPEVRLVDGGTQGLALLPVVGEAGLL
CCCCCEEEEECCEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEECCCCEE
VILDAVDFGLAPGTLMVAADDDVPRYLTAKKLSLHQTSFQDVLALAQISGRAPARMVLVG
EEEEEHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
VQPCDLDTYGGGLSAPVAARIGEAVDLVRAHLAGAGFALAPRAAGEEAEPLGPAAVAMTF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEC
GGPAAQIAEAS
CCCHHHCCCCC
>Mature Secondary Structure 
SRGATVLGIGNVLWADEGFGVRAVEALNAGWTCGPEVRLVDGGTQGLALLPVVGEAGLL
CCCCEEEEECCEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEECCCCEE
VILDAVDFGLAPGTLMVAADDDVPRYLTAKKLSLHQTSFQDVLALAQISGRAPARMVLVG
EEEEEHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
VQPCDLDTYGGGLSAPVAARIGEAVDLVRAHLAGAGFALAPRAAGEEAEPLGPAAVAMTF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEC
GGPAAQIAEAS
CCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8125335 [H]