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The map label for this gene is hupZ [H]

Identifier: 148259355

GI number: 148259355

Start: 385002

End: 385736

Strand: Reverse

Name: hupZ [H]

Synonym: Acry_0336

Alternate gene names: 148259355

Gene position: 385736-385002 (Counterclockwise)

Preceding gene: 148259356

Following gene: 148259354

Centisome position: 11.38

GC content: 63.54

Gene sequence:

>735_bases
ATGACGGCAGACACGCAGACGACGCCTACGGATCTCGCGCAGGGGGCGACGGCGAGGCGGGTGAGCGCGCCGGTCTATGT
GTACGAGCTGCCGGTGCGGATCTGGCACTGGGTGATGGCGCTGTCGATCGTGATTTTGTCGATCACCGGATTCCTGATCG
CCGATCCGCTGTGGCCGTTGCAGGTCGGCCAGCCGAGCCAGCATTTCCTGATGGGCGACATCCGCACGATCCATTTCATC
ACCGGCGACGTGTTCGCGGTGGCGATGCTGGTGCGGATCTACTGGGCGATCGTCGGCAACCGGTATGCGCGGGAGATTTT
CGCGCCCGCGGTCTGGCGGCGGTCGTGGCGGCGCGAGTTCGCCAGGACGATCCGCTGGTACCTGTTCCTCGAACGCGAGA
CGAGCAAGGAGATCGGGCATAATCCGCTGGCGCAGCTTGCGATGTTCGCGATGTTCACGCTCGGCGTGCTGTTCCAGATC
GTCACCGGCTTCGCGCTGTTCGGCGAGGGGACCGGCCATGGCACCTGGGCCTTTGCGCTGTTCACGAGCTGGGTGATGCC
GCTCTTCGGCAACAGCCAGGCGGTGCACACGTGGCATCACCTGGCGATGTGGTACCTGATCCTGTTCGCGATGGTCCATG
TCTACATGTCGGTGCGGGAGGACATCATGTCGCGGCAGACGATGATCAGCACGATGATCAATGGCTGGCGCTTCTTCAAG
GACGACCGGGAATGA

Upstream 100 bases:

>100_bases
CCTCGCCTGCTCGACGCATGTGATGGCGCCGGATGGCGGCGAGCTGGTGCGCGTGCAGGTGCTCTGAGGGATCGTCGGAA
ACAGGATCGGGGATCGTGCC

Downstream 100 bases:

>100_bases
GCCGCGGGGCGACGGTGCTCGGGATCGGTAACGTGCTCTGGGCGGACGAGGGGTTCGGCGTGCGCGCGGTGGAGGCGCTG
AATGCCGGCTGGACGTGCGG

Product: Ni/Fe-hydrogenase, b-type cytochrome subunit

Products: reduced ferredoxin; H+

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTADTQTTPTDLAQGATARRVSAPVYVYELPVRIWHWVMALSIVILSITGFLIADPLWPLQVGQPSQHFLMGDIRTIHFI
TGDVFAVAMLVRIYWAIVGNRYAREIFAPAVWRRSWRREFARTIRWYLFLERETSKEIGHNPLAQLAMFAMFTLGVLFQI
VTGFALFGEGTGHGTWAFALFTSWVMPLFGNSQAVHTWHHLAMWYLILFAMVHVYMSVREDIMSRQTMISTMINGWRFFK
DDRE

Sequences:

>Translated_244_residues
MTADTQTTPTDLAQGATARRVSAPVYVYELPVRIWHWVMALSIVILSITGFLIADPLWPLQVGQPSQHFLMGDIRTIHFI
TGDVFAVAMLVRIYWAIVGNRYAREIFAPAVWRRSWRREFARTIRWYLFLERETSKEIGHNPLAQLAMFAMFTLGVLFQI
VTGFALFGEGTGHGTWAFALFTSWVMPLFGNSQAVHTWHHLAMWYLILFAMVHVYMSVREDIMSRQTMISTMINGWRFFK
DDRE
>Mature_243_residues
TADTQTTPTDLAQGATARRVSAPVYVYELPVRIWHWVMALSIVILSITGFLIADPLWPLQVGQPSQHFLMGDIRTIHFIT
GDVFAVAMLVRIYWAIVGNRYAREIFAPAVWRRSWRREFARTIRWYLFLERETSKEIGHNPLAQLAMFAMFTLGVLFQIV
TGFALFGEGTGHGTWAFALFTSWVMPLFGNSQAVHTWHHLAMWYLILFAMVHVYMSVREDIMSRQTMISTMINGWRFFKD
DRE

Specific function: Probable b-type cytochrome [H]

COG id: COG1969

COG function: function code C; Ni,Fe-hydrogenase I cytochrome b subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupC/hyaC/hydC family [H]

Homologues:

Organism=Escherichia coli, GI1787208, Length=219, Percent_Identity=50.6849315068493, Blast_Score=209, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR000516 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.12.7.2

Molecular weight: Translated: 28284; Mature: 28153

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00882 NI_HGENASE_CYTB_1 ; PS00883 NI_HGENASE_CYTB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADTQTTPTDLAQGATARRVSAPVYVYELPVRIWHWVMALSIVILSITGFLIADPLWPL
CCCCCCCCHHHHHCCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QVGQPSQHFLMGDIRTIHFITGDVFAVAMLVRIYWAIVGNRYAREIFAPAVWRRSWRREF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARTIRWYLFLERETSKEIGHNPLAQLAMFAMFTLGVLFQIVTGFALFGEGTGHGTWAFAL
HHHHHHHHEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FTSWVMPLFGNSQAVHTWHHLAMWYLILFAMVHVYMSVREDIMSRQTMISTMINGWRFFK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDRE
CCCC
>Mature Secondary Structure 
TADTQTTPTDLAQGATARRVSAPVYVYELPVRIWHWVMALSIVILSITGFLIADPLWPL
CCCCCCCHHHHHCCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QVGQPSQHFLMGDIRTIHFITGDVFAVAMLVRIYWAIVGNRYAREIFAPAVWRRSWRREF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARTIRWYLFLERETSKEIGHNPLAQLAMFAMFTLGVLFQIVTGFALFGEGTGHGTWAFAL
HHHHHHHHEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
FTSWVMPLFGNSQAVHTWHHLAMWYLILFAMVHVYMSVREDIMSRQTMISTMINGWRFFK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDRE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; oxidized ferredoxin

Specific reaction: H2 + oxidized ferredoxin = reduced ferredoxin + 2 H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7966281 [H]