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The map label for this gene is gatA [H]

Identifier: 148259263

GI number: 148259263

Start: 278928

End: 280097

Strand: Reverse

Name: gatA [H]

Synonym: Acry_0243

Alternate gene names: 148259263

Gene position: 280097-278928 (Counterclockwise)

Preceding gene: 148259266

Following gene: 148259258

Centisome position: 8.26

GC content: 72.31

Gene sequence:

>1170_bases
ATGGAAACGCGCCATCCCCTCTCCGAAGCTCCCGATCTCGGCCTGATCAAATCGCTCGACATCCGGGGCAATCCGCACGG
CCCGCTTGCGGGGCTCGACGTGGTCGTCAAGGACCTGTTCGACATCGCGGGCGAAGTGACCGCCTTCGGCAACCCCGACT
GGGGGCGGCATTTCGGCCCCGCCCTCTCGCATGCCTGGATCGTCTCACAGCTTCTCGAGGCCGGCGCCCGCGTCACCGGC
AAGACAACGACGGTCGAACTCGCCTTCGGCCTGGAGGGGCGCAACACCCATTACGGCACGCCGCTCAACCCGGCGGCGCC
GGACCGGCTGCCGGGCGGCTCGTCGAGCGGCTCGGTGGCGATGGTCGCGTCCGGCCGCGCCCATGTCGGCGTCGGCTCGG
ATACCGGCGGCTCGGTGCGGATTCCGGCCAGCTATTGCGGGCTCTACGGGTTGCGCCCGACTCAGGGGCTGCTCAGCCTG
GCCGGGGCGGCGCCGCTGGCCCCGAGCTTCGACACGCCTGGCTGGTTCACCCGCGATGCGGAGACCATGCTTCGCGTCGG
CGAGGCGCTTTTGCCGCCAGCACCGCCGCTCGCGGCCCAGTTTCTCATGATTCCCGCAGCCTTCGCCAATGCCGATCCCG
ACGTCGTCACTGCCCTCGAACCCGCGCTCGCCAGGCTGGGCCCGATGGGCGAGGCCGACCCGGTGCCGGAAGGGCTCGAC
CGGATGCTGGCCTGGCAGAACGCCGTGCGCGGGCGCGAGACGTGGCTGACGCTGGGCGGCTTCATCGAGACGGTCAAGCC
GCTGCTCGACCCCGTCACCGCGGGCCGGATCGCGGCGACGCGCGGCTTCACCGCCGAAGCCGCTGCCGAGGGACGCGTGG
CGCGCCGCGCGCACACGGCGCGGATGCACGCGCTGCTGGCCGGCGGCGCGGTGCTGGTGATTCCGACCTCGCCCTGCCCG
GCCCCGCCGGTTGCGGCGGAGCAGGCGGTCTATGAAGACGTCCGCACGCGAACGCTGCGCGTCGGTATCATCGCCGCCTT
CGCCGGCCTGCCGGAACTCACCATTCCGGTCGGCAAGGTGAACGGGGCGCCGGTCGGCCTGTCGCTCATCGCCGGGCCGG
GACGCGACCTCGCCCTGCTCGAACTCGCGGCCGACCTCGCACTCGCTTGA

Upstream 100 bases:

>100_bases
TCGTCCTTGTCCTCTGCCGGAGATATCGGTGCCGGCCAGTCGATTTTAACCCTGCCGGAAGCCGGTTCAACCCGGCGCCG
AACTCTGTTACCCTTTCGCC

Downstream 100 bases:

>100_bases
GCGCGGGGCCGGAACGCGAATCCAGAAGCGCTTCAGGGACGCGACGTCAAAAGATCTCCTCGTAGATCTTCACGATCTCG
GCAGCGTCCGGCACCGCCGG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
METRHPLSEAPDLGLIKSLDIRGNPHGPLAGLDVVVKDLFDIAGEVTAFGNPDWGRHFGPALSHAWIVSQLLEAGARVTG
KTTTVELAFGLEGRNTHYGTPLNPAAPDRLPGGSSSGSVAMVASGRAHVGVGSDTGGSVRIPASYCGLYGLRPTQGLLSL
AGAAPLAPSFDTPGWFTRDAETMLRVGEALLPPAPPLAAQFLMIPAAFANADPDVVTALEPALARLGPMGEADPVPEGLD
RMLAWQNAVRGRETWLTLGGFIETVKPLLDPVTAGRIAATRGFTAEAAAEGRVARRAHTARMHALLAGGAVLVIPTSPCP
APPVAAEQAVYEDVRTRTLRVGIIAAFAGLPELTIPVGKVNGAPVGLSLIAGPGRDLALLELAADLALA

Sequences:

>Translated_389_residues
METRHPLSEAPDLGLIKSLDIRGNPHGPLAGLDVVVKDLFDIAGEVTAFGNPDWGRHFGPALSHAWIVSQLLEAGARVTG
KTTTVELAFGLEGRNTHYGTPLNPAAPDRLPGGSSSGSVAMVASGRAHVGVGSDTGGSVRIPASYCGLYGLRPTQGLLSL
AGAAPLAPSFDTPGWFTRDAETMLRVGEALLPPAPPLAAQFLMIPAAFANADPDVVTALEPALARLGPMGEADPVPEGLD
RMLAWQNAVRGRETWLTLGGFIETVKPLLDPVTAGRIAATRGFTAEAAAEGRVARRAHTARMHALLAGGAVLVIPTSPCP
APPVAAEQAVYEDVRTRTLRVGIIAAFAGLPELTIPVGKVNGAPVGLSLIAGPGRDLALLELAADLALA
>Mature_389_residues
METRHPLSEAPDLGLIKSLDIRGNPHGPLAGLDVVVKDLFDIAGEVTAFGNPDWGRHFGPALSHAWIVSQLLEAGARVTG
KTTTVELAFGLEGRNTHYGTPLNPAAPDRLPGGSSSGSVAMVASGRAHVGVGSDTGGSVRIPASYCGLYGLRPTQGLLSL
AGAAPLAPSFDTPGWFTRDAETMLRVGEALLPPAPPLAAQFLMIPAAFANADPDVVTALEPALARLGPMGEADPVPEGLD
RMLAWQNAVRGRETWLTLGGFIETVKPLLDPVTAGRIAATRGFTAEAAAEGRVARRAHTARMHALLAGGAVLVIPTSPCP
APPVAAEQAVYEDVRTRTLRVGIIAAFAGLPELTIPVGKVNGAPVGLSLIAGPGRDLALLELAADLALA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=437, Percent_Identity=25.4004576659039, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI195972892, Length=140, Percent_Identity=35.7142857142857, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=406, Percent_Identity=25.1231527093596, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=169, Percent_Identity=33.7278106508876, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=179, Percent_Identity=32.9608938547486, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=99, Percent_Identity=43.4343434343434, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=388, Percent_Identity=30.1546391752577, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=129, Percent_Identity=36.4341085271318, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21356731, Length=410, Percent_Identity=25.1219512195122, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648113, Length=161, Percent_Identity=39.7515527950311, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161078093, Length=421, Percent_Identity=25.1781472684086, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550774, Length=152, Percent_Identity=33.5526315789474, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648435, Length=151, Percent_Identity=35.7615894039735, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648437, Length=151, Percent_Identity=35.7615894039735, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648441, Length=151, Percent_Identity=35.7615894039735, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648439, Length=151, Percent_Identity=35.7615894039735, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644968, Length=359, Percent_Identity=23.6768802228412, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652985, Length=378, Percent_Identity=24.3386243386243, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922090, Length=378, Percent_Identity=24.3386243386243, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652981, Length=378, Percent_Identity=24.3386243386243, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652983, Length=378, Percent_Identity=24.3386243386243, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 40050; Mature: 40050

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRHPLSEAPDLGLIKSLDIRGNPHGPLAGLDVVVKDLFDIAGEVTAFGNPDWGRHFGP
CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHCH
ALSHAWIVSQLLEAGARVTGKTTTVELAFGLEGRNTHYGTPLNPAAPDRLPGGSSSGSVA
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
MVASGRAHVGVGSDTGGSVRIPASYCGLYGLRPTQGLLSLAGAAPLAPSFDTPGWFTRDA
EEECCCEEEECCCCCCCEEEECHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCH
ETMLRVGEALLPPAPPLAAQFLMIPAAFANADPDVVTALEPALARLGPMGEADPVPEGLD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
RMLAWQNAVRGRETWLTLGGFIETVKPLLDPVTAGRIAATRGFTAEAAAEGRVARRAHTA
HHHHHHHHHCCHHHEEEHHHHHHHHHHHHCHHCCCCEECCCCCCHHHHHCCHHHHHHHHH
RMHALLAGGAVLVIPTSPCPAPPVAAEQAVYEDVRTRTLRVGIIAAFAGLPELTIPVGKV
HHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
NGAPVGLSLIAGPGRDLALLELAADLALA
CCCCCEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
METRHPLSEAPDLGLIKSLDIRGNPHGPLAGLDVVVKDLFDIAGEVTAFGNPDWGRHFGP
CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHCH
ALSHAWIVSQLLEAGARVTGKTTTVELAFGLEGRNTHYGTPLNPAAPDRLPGGSSSGSVA
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
MVASGRAHVGVGSDTGGSVRIPASYCGLYGLRPTQGLLSLAGAAPLAPSFDTPGWFTRDA
EEECCCEEEECCCCCCCEEEECHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCH
ETMLRVGEALLPPAPPLAAQFLMIPAAFANADPDVVTALEPALARLGPMGEADPVPEGLD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
RMLAWQNAVRGRETWLTLGGFIETVKPLLDPVTAGRIAATRGFTAEAAAEGRVARRAHTA
HHHHHHHHHCCHHHEEEHHHHHHHHHHHHCHHCCCCEECCCCCCHHHHHCCHHHHHHHHH
RMHALLAGGAVLVIPTSPCPAPPVAAEQAVYEDVRTRTLRVGIIAAFAGLPELTIPVGKV
HHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
NGAPVGLSLIAGPGRDLALLELAADLALA
CCCCCEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA