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The map label for this gene is leuD [H]

Identifier: 148243431

GI number: 148243431

Start: 2029111

End: 2029734

Strand: Direct

Name: leuD [H]

Synonym: SynRCC307_2332

Alternate gene names: 148243431

Gene position: 2029111-2029734 (Clockwise)

Preceding gene: 148243430

Following gene: 148243432

Centisome position: 91.2

GC content: 60.9

Gene sequence:

>624_bases
ATGACATCAACTCCTTTTCCCAGCGGCGCAATCAGCCAAGTCAGCGGACGCACCGTGGTGCTGCGCGGCAACGACATCGA
CACCGACCGAATCATCCCGGCGCGCTTTCTCAAATGCGTGACCTTCGATGGCCTTGGCGCCCAGGTGTTCGCCGACGATC
GCAGCGAGCAAAACGGTGAACACCCCTTTGATCGACCTGAGCACCAAGGGGCAGCTGTCTTGATCGTGAACCGCAACTTC
GGCTGCGGCTCCAGCCGTGAACATGCCCCCCAAGCGCTATTGCGTTGGGGGATCCGCGGCGTGGTGGGCGAAAGTTTCGC
CGAAATCTTCTATGGAAACTGCCTGGCTTTAGGCATTCCCTGCCTGCAGGCCAGCAGCAAGGAGATCGAGGCTCTGCAAA
CGGCTGCTGAAGCCGCTCCGCAAAGCAGCGCCAGCATCAGCTTGGAGCCTGCTGCGATCAAGATCGATGGCCGTAACTGG
GATCTGAGCATGGCGGCGGATGCCAAAAATCGACTGCTGAACGGCCAGTGGGATGCCACCAGCCAGCTGCTGCAACAGGA
GCAAGCACTCCAAGACACAGCGGCGCGCTTGCCCTACATGCAGGGGTTCGTGGCCAGCCGCTGA

Upstream 100 bases:

>100_bases
CAGCGGCCGCACGCTGCTGATGAGCCCGGCGATGGTGGCTGCCGCTGCCATTGCGGGCCGTGTCACCGACGTTCGCTCCC
TGCCGCCTGCTTAAACACCC

Downstream 100 bases:

>100_bases
TTATTTGTCTACAAAAGAGATAGCGATGAGACGGATGCGATGGACCCAGTGCCTGCCCCCCGCCATCGCTCCCGCTGGAG
CTTGTTGAGCCTGCTGGTGT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MTSTPFPSGAISQVSGRTVVLRGNDIDTDRIIPARFLKCVTFDGLGAQVFADDRSEQNGEHPFDRPEHQGAAVLIVNRNF
GCGSSREHAPQALLRWGIRGVVGESFAEIFYGNCLALGIPCLQASSKEIEALQTAAEAAPQSSASISLEPAAIKIDGRNW
DLSMAADAKNRLLNGQWDATSQLLQQEQALQDTAARLPYMQGFVASR

Sequences:

>Translated_207_residues
MTSTPFPSGAISQVSGRTVVLRGNDIDTDRIIPARFLKCVTFDGLGAQVFADDRSEQNGEHPFDRPEHQGAAVLIVNRNF
GCGSSREHAPQALLRWGIRGVVGESFAEIFYGNCLALGIPCLQASSKEIEALQTAAEAAPQSSASISLEPAAIKIDGRNW
DLSMAADAKNRLLNGQWDATSQLLQQEQALQDTAARLPYMQGFVASR
>Mature_206_residues
TSTPFPSGAISQVSGRTVVLRGNDIDTDRIIPARFLKCVTFDGLGAQVFADDRSEQNGEHPFDRPEHQGAAVLIVNRNFG
CGSSREHAPQALLRWGIRGVVGESFAEIFYGNCLALGIPCLQASSKEIEALQTAAEAAPQSSASISLEPAAIKIDGRNWD
LSMAADAKNRLLNGQWDATSQLLQQEQALQDTAARLPYMQGFVASR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=190, Percent_Identity=35.2631578947368, Blast_Score=119, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321429, Length=114, Percent_Identity=35.0877192982456, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22316; Mature: 22185

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTPFPSGAISQVSGRTVVLRGNDIDTDRIIPARFLKCVTFDGLGAQVFADDRSEQNGE
CCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCC
HPFDRPEHQGAAVLIVNRNFGCGSSREHAPQALLRWGIRGVVGESFAEIFYGNCLALGIP
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCH
CLQASSKEIEALQTAAEAAPQSSASISLEPAAIKIDGRNWDLSMAADAKNRLLNGQWDAT
HHCCCHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCCCEEEECCCHHHHCCCCCCCHH
SQLLQQEQALQDTAARLPYMQGFVASR
HHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
TSTPFPSGAISQVSGRTVVLRGNDIDTDRIIPARFLKCVTFDGLGAQVFADDRSEQNGE
CCCCCCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCC
HPFDRPEHQGAAVLIVNRNFGCGSSREHAPQALLRWGIRGVVGESFAEIFYGNCLALGIP
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCH
CLQASSKEIEALQTAAEAAPQSSASISLEPAAIKIDGRNWDLSMAADAKNRLLNGQWDAT
HHCCCHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCCCEEEECCCHHHHCCCCCCCHH
SQLLQQEQALQDTAARLPYMQGFVASR
HHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]