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The map label for this gene is cobS [H]

Identifier: 148243411

GI number: 148243411

Start: 2010789

End: 2011544

Strand: Direct

Name: cobS [H]

Synonym: SynRCC307_2312

Alternate gene names: 148243411

Gene position: 2010789-2011544 (Clockwise)

Preceding gene: 148243408

Following gene: 148243413

Centisome position: 90.38

GC content: 68.12

Gene sequence:

>756_bases
ATGCTCAGGTGGTGGCCGGATCTGGCCGGAAGCTGGATCTTCTACACAACGCTCCCGCTCTGGCCGGGGGTGACCCCGCG
CTTTGAGCGGATTGCCCGGTTTGCTCCGGTGATTGGCGTCTTCATTGGTGGCGCCCAAGCTGGCCTCTGGCTGCTGGGCC
GCGAACTGGGGCTGCCCAGCGCCAGCTGCGCCCTACTGGTGCTGGCCCTGGGGCTTTGGCTCACCGGCGGCTTGCACCTC
GATGGGGTGATGGATACCGGAGATGGCTTGGCCGCCGGCCCCCGCTGCCTAGCGGCCATGGCCGACAGCCGCATCGGCGC
CAGCGGCTTGGTGGCTGGCCTGATGGTGCTGCTGCTCAAGTTGGGTGCCCTGCTGGCCCTCGCGGCCTTGGCACCCACCC
TGTTGATCTGGAGTGCGGTGTGGGGCCGCATTGCACCACTGCTGGCCATCGCCTGGTTCCCCTATCTCAGGGCCGAAGGC
AGTGGTGGCTTCCACCGGCTCCAGCGTCAACCCCTGCTGCAAGAACTGCTGCCGGCAGCGGCTGTGCTGGGGCTGCTGAT
AGGACTCAGCCTCAGCCCCCCCGGCACGCGCACGGTGCTGGCTGGGGTTTGCGGTGTGATCCCAACGCTGCTGGTGCCTC
GGCTGCTGGGCCAACGCCTGGGAGGACACACCGGCGATAGCTACGGCGCCTGCGTGGAATGGACCGAAGCCTGCACGCTG
TGGAGCGGCTGGCTGATGCTGCGTCTGCTCAGCTGA

Upstream 100 bases:

>100_bases
GCAGCTCGAATTGGAACGGAGCAGCAGTGCTCAACACCCACAGACAGCGCAACTCTCACCCTATGGCGAGGCGCAGGATG
GAGGCAGCACCCAACCGCTG

Downstream 100 bases:

>100_bases
GCGGCAGCGTCAACACAAACGCGTTCCCCGCAGCCGCCAAACCAGGCGCTAAAGCACTGGCAGGACAGTGCAGCTGCAGA
CTGCCGCCGCTGGCTTCGGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLRWWPDLAGSWIFYTTLPLWPGVTPRFERIARFAPVIGVFIGGAQAGLWLLGRELGLPSASCALLVLALGLWLTGGLHL
DGVMDTGDGLAAGPRCLAAMADSRIGASGLVAGLMVLLLKLGALLALAALAPTLLIWSAVWGRIAPLLAIAWFPYLRAEG
SGGFHRLQRQPLLQELLPAAAVLGLLIGLSLSPPGTRTVLAGVCGVIPTLLVPRLLGQRLGGHTGDSYGACVEWTEACTL
WSGWLMLRLLS

Sequences:

>Translated_251_residues
MLRWWPDLAGSWIFYTTLPLWPGVTPRFERIARFAPVIGVFIGGAQAGLWLLGRELGLPSASCALLVLALGLWLTGGLHL
DGVMDTGDGLAAGPRCLAAMADSRIGASGLVAGLMVLLLKLGALLALAALAPTLLIWSAVWGRIAPLLAIAWFPYLRAEG
SGGFHRLQRQPLLQELLPAAAVLGLLIGLSLSPPGTRTVLAGVCGVIPTLLVPRLLGQRLGGHTGDSYGACVEWTEACTL
WSGWLMLRLLS
>Mature_251_residues
MLRWWPDLAGSWIFYTTLPLWPGVTPRFERIARFAPVIGVFIGGAQAGLWLLGRELGLPSASCALLVLALGLWLTGGLHL
DGVMDTGDGLAAGPRCLAAMADSRIGASGLVAGLMVLLLKLGALLALAALAPTLLIWSAVWGRIAPLLAIAWFPYLRAEG
SGGFHRLQRQPLLQELLPAAAVLGLLIGLSLSPPGTRTVLAGVCGVIPTLLVPRLLGQRLGGHTGDSYGACVEWTEACTL
WSGWLMLRLLS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=243, Percent_Identity=31.2757201646091, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26484; Mature: 26484

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRWWPDLAGSWIFYTTLPLWPGVTPRFERIARFAPVIGVFIGGAQAGLWLLGRELGLPS
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
ASCALLVLALGLWLTGGLHLDGVMDTGDGLAAGPRCLAAMADSRIGASGLVAGLMVLLLK
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
LGALLALAALAPTLLIWSAVWGRIAPLLAIAWFPYLRAEGSGGFHRLQRQPLLQELLPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AVLGLLIGLSLSPPGTRTVLAGVCGVIPTLLVPRLLGQRLGGHTGDSYGACVEWTEACTL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
WSGWLMLRLLS
HHHHHHHHHCC
>Mature Secondary Structure
MLRWWPDLAGSWIFYTTLPLWPGVTPRFERIARFAPVIGVFIGGAQAGLWLLGRELGLPS
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
ASCALLVLALGLWLTGGLHLDGVMDTGDGLAAGPRCLAAMADSRIGASGLVAGLMVLLLK
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
LGALLALAALAPTLLIWSAVWGRIAPLLAIAWFPYLRAEGSGGFHRLQRQPLLQELLPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AVLGLLIGLSLSPPGTRTVLAGVCGVIPTLLVPRLLGQRLGGHTGDSYGACVEWTEACTL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
WSGWLMLRLLS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]