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The map label for this gene is fabI [H]

Identifier: 148243388

GI number: 148243388

Start: 1987884

End: 1988666

Strand: Direct

Name: fabI [H]

Synonym: SynRCC307_2289

Alternate gene names: 148243388

Gene position: 1987884-1988666 (Clockwise)

Preceding gene: 148243385

Following gene: 148243389

Centisome position: 89.35

GC content: 63.09

Gene sequence:

>783_bases
ATGCTGCTCGATCTCACCGGCAAGAAGATCCTCGTTACCGGCATCGCCAATAACCGCTCCATCGCTTGGGGCATTGCCCA
GCAACTCAAGGCCGCTGGCGCTGAGCTGGGCATCACCTACCTGCCCGATGACAAGGGCCGCTTTGAGGCCAAGGTGCGTG
AACTCACCGAACCCCTAGCGCCCAGCCTGTTTCTGCCACTCAACGTGCAAGACCCCGCCCAGATGGAGGCGGTGTTCAAC
GAAATCAAGGAGAAGTGGGGCGTGCTGGATGGCCTGGTGCACTGCCTGGCCTTTGCCGGCAAAGAAGAATTGATTGGCGA
CTACAGCGCCACCACCGCCGCCGGATTTGCCCGGGCCCTGGAGATCAGCGCCTACTCCTTGGCGCCGCTCTGCGCCTACG
CCAAGCCGCTCTTCAGCGAAAAAGCTGGCGTGATCACCCTCTCCTACCTGGGGGCCGAACGGGCGATCCCCAACTACAAC
GTCATGGGCGTGGCAAAGGCCGCCCTGGAAGCGTCCGTCCGTTATCTGGCCGCCGAACTGGGCCCTGAAAAGCAGGTGCG
CGTCAACGCCATCAGCGCTGGCCCGATCCGCACCCTGGCGAGCTCCGCCATCGGCGGCATCCTCGACATGATCCACAACG
TGGAAGAGAAAGCTCCGCTGCGCCGCACCGTGACCCAGATGGAAGTGGGCGGCACCGCCGCCTTCCTGCTGAGTGATCTG
GCCAGTGGCATCTCCGGGCAAACCATCTATGTGGATGCCGGCTACTGCGTCAACGGCATGTGA

Upstream 100 bases:

>100_bases
AGAAGAAGCAGCGGGCACCGGAGCGAGCTGGTCGACTAAACGCCGATCTTGGCGGGGCATGGGGGAGAATCGCGCGATTC
CGCCCTGCAGGACCGCCCCG

Downstream 100 bases:

>100_bases
GCCTGGACCGATCGCCCAGGCCTGTCAGCATGGGGTGAATGGCGATCGGGCATGGGCCGGCAAGGAAACATCCATCGCGT
CACCGGAGAAACCGAGGTCC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLLDLTGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLPDDKGRFEAKVRELTEPLAPSLFLPLNVQDPAQMEAVFN
EIKEKWGVLDGLVHCLAFAGKEELIGDYSATTAAGFARALEISAYSLAPLCAYAKPLFSEKAGVITLSYLGAERAIPNYN
VMGVAKAALEASVRYLAAELGPEKQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQMEVGGTAAFLLSDL
ASGISGQTIYVDAGYCVNGM

Sequences:

>Translated_260_residues
MLLDLTGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLPDDKGRFEAKVRELTEPLAPSLFLPLNVQDPAQMEAVFN
EIKEKWGVLDGLVHCLAFAGKEELIGDYSATTAAGFARALEISAYSLAPLCAYAKPLFSEKAGVITLSYLGAERAIPNYN
VMGVAKAALEASVRYLAAELGPEKQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQMEVGGTAAFLLSDL
ASGISGQTIYVDAGYCVNGM
>Mature_260_residues
MLLDLTGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLPDDKGRFEAKVRELTEPLAPSLFLPLNVQDPAQMEAVFN
EIKEKWGVLDGLVHCLAFAGKEELIGDYSATTAAGFARALEISAYSLAPLCAYAKPLFSEKAGVITLSYLGAERAIPNYN
VMGVAKAALEASVRYLAAELGPEKQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQMEVGGTAAFLLSDL
ASGISGQTIYVDAGYCVNGM

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=48.2490272373541, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1789378, Length=243, Percent_Identity=26.7489711934156, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24643142, Length=269, Percent_Identity=26.3940520446097, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27531; Mature: 27531

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDLTGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLPDDKGRFEAKVRELTEPLA
CEEECCCCEEEEEECCCCCEEHHHHHHHHHHCCHHCCEEECCCCCCHHHHHHHHHHHHCC
PSLFLPLNVQDPAQMEAVFNEIKEKWGVLDGLVHCLAFAGKEELIGDYSATTAAGFARAL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHH
EISAYSLAPLCAYAKPLFSEKAGVITLSYLGAERAIPNYNVMGVAKAALEASVRYLAAEL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
GPEKQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQMEVGGTAAFLLSDL
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ASGISGQTIYVDAGYCVNGM
HCCCCCCEEEEECCEEECCC
>Mature Secondary Structure
MLLDLTGKKILVTGIANNRSIAWGIAQQLKAAGAELGITYLPDDKGRFEAKVRELTEPLA
CEEECCCCEEEEEECCCCCEEHHHHHHHHHHCCHHCCEEECCCCCCHHHHHHHHHHHHCC
PSLFLPLNVQDPAQMEAVFNEIKEKWGVLDGLVHCLAFAGKEELIGDYSATTAAGFARAL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHH
EISAYSLAPLCAYAKPLFSEKAGVITLSYLGAERAIPNYNVMGVAKAALEASVRYLAAEL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
GPEKQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQMEVGGTAAFLLSDL
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ASGISGQTIYVDAGYCVNGM
HCCCCCCEEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8391534; 11759840 [H]