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The map label for this gene is slt [H]

Identifier: 148243320

GI number: 148243320

Start: 1927597

End: 1929330

Strand: Direct

Name: slt [H]

Synonym: SynRCC307_2221

Alternate gene names: 148243320

Gene position: 1927597-1929330 (Clockwise)

Preceding gene: 148243317

Following gene: 148243323

Centisome position: 86.64

GC content: 65.69

Gene sequence:

>1734_bases
ATGCAGCTGCGGGGTGGAGGAGTGCTGCTGATCAGCATCGCCGCGGCCGGCACCGTGGTCATAAGCAGTGCCTTGCAGCA
GGGCCTGCAACAAATCCGACCTGAACTCGACCGCCACAGCTCACCCACCCTGCTGCGGCGAACCGCTCGCTTTGATCCCG
ACCCAAGCCGGCGACGCCTCGCGCGGCTGATCCTGGCGAACCAGGGCGATGTCAGCGCCTCCAGGCAGCAATGGTTGCTG
CACGCTCAAGGGTGGGGCGACAACCCACTGAGCCCCGTGGTGCTCAAACAACAAGCTCTAGCGGCGCGGGCGCTCGGCCA
ACAGGAGCGATCCGATCGCCGCTGGACAGAGCTGTTGCAACGCTTCCCAGCTGCAGCACCCAGCGCCGACGCCCTCTACT
ACCTGGGCCAGCCAGGAGATGAGCAGCACCAGGAACTGCTGCAGCGCTTTGCGGCCCACCCCGCGGCCCTGGCCGCTGCA
GTGGACTGGGACCGCCAAACGCAAGATCCCCGCGCCGGGTTGCATCTGGCTGCTTGGGGAGCGCGCTGGCCAGGAGCCGA
TGCAGCCCTTCAGCGCGCCTGCCTTCGTGATCTCGGCGATCAACAGCGCGAGCAAGTGGCCACGGCCCTGGCCCAACAGG
GGGATCTCGATGCGGCCCTGAACTGCCTTGGCGATCTGAGGCCCACAGCGCCCAAGACCCTGCAATGGCTTGGGCTGGCC
GCTGAGGCGCCCTTGAGCCCTGAACAACAGGCCTGGGAGCAGAGCCGTTCCCTCCTGTTGGAGCGCGATTGGAATGGCGC
CCAAGAGGCGCTGCAGCAACAACTGGAGCAACCCCTAACGCCTCCCCTGCTGGCACGGGTGCGCTTCTGGCTTGGCCTCA
GCGCCTGGGAGCTGGGCGACGCCGAGCAGGCGCGGCAAATCTGGCAGCAGCTGTTACGCGAGCACCCCTTTGGCTACTAC
GGCTGGCGCGCCAGTGAACGGCTGCAGCAGGCCCCTGATGCGCTGCCGCTTGAAAGCCCTCCGGCGGAAGGCTTGCTGCC
CAAGGACTTGGATCAATTGCTCGCGCTCGGGATTCCCGTTGAGGCCTGGGAGCAGTGGCGCATGCAACGGGGCGGCCGAC
CAGCGGAATCGCCCAGAGAGCTTTGGCAAGAAGGCGTCCTGCGTCTGGGGGTTGGCGATCGCTGGATCGCGTTAGAGCAA
CTGGACCGGGCCCAGCTGCTTGGTGCCGCTGAGGGCTTGAGCCAACAGGTGCTACTGGAGCAACACCGCCATCCGCGCAA
CTTTGAACAACTCCTGGACGAGGCCGCGGGCAACGAGCAACTCGACCCTGATCTGCTCTTGGGCTTGGCCCGGCAAGAGT
CGCGGCTGACGCCCACGGTGCGCTCCAGCGCTGGAGCCATCGGCCTGCTGCAGCTGCTGCCCAGCACCGCTGCTGAGCTG
GACGATCCAGCCCCAAGCGAAGACGACTTACTGCAACCGGAGCGCAATGCCCCCTTGGGTGCCCGCTATCTCAAAGAGAT
GCTGAACGCTGCAGACGGCAATCCCTTTCTGGCAACAGCCAGCTACAACGCGGGACCCGGTGCTGCCGGCAGCTGGCGCA
ACGACGATCTCGATGCCATCCCTGAACTCTGGGTGGAAGCCATCCCCTATCCCGAGACCCGGATCTACGTGAAAAAAGTG
CTGGGCAACCGCTGGACCTATGCCTTGCTCGGCGACAGGTTGACCAACCACTGA

Upstream 100 bases:

>100_bases
CAGAACGAAAGCGCTCCCATCGCTTGACCATGGTTCCCGCCGGGCTGCAGCCGCGCCATTGTGACCTCCATACGATGCCG
GCAAAGGACAACGGAGGTTG

Downstream 100 bases:

>100_bases
AACCATTCCATGCCGCCCAACTGGTTGTGAATGGAGTGGCCGTAGGTCATGAACAACAGGATGTAAGCGTTGAAACCAAT
CAGGCCGAGCCAGGCCGGCG

Product: soluble lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MQLRGGGVLLISIAAAGTVVISSALQQGLQQIRPELDRHSSPTLLRRTARFDPDPSRRRLARLILANQGDVSASRQQWLL
HAQGWGDNPLSPVVLKQQALAARALGQQERSDRRWTELLQRFPAAAPSADALYYLGQPGDEQHQELLQRFAAHPAALAAA
VDWDRQTQDPRAGLHLAAWGARWPGADAALQRACLRDLGDQQREQVATALAQQGDLDAALNCLGDLRPTAPKTLQWLGLA
AEAPLSPEQQAWEQSRSLLLERDWNGAQEALQQQLEQPLTPPLLARVRFWLGLSAWELGDAEQARQIWQQLLREHPFGYY
GWRASERLQQAPDALPLESPPAEGLLPKDLDQLLALGIPVEAWEQWRMQRGGRPAESPRELWQEGVLRLGVGDRWIALEQ
LDRAQLLGAAEGLSQQVLLEQHRHPRNFEQLLDEAAGNEQLDPDLLLGLARQESRLTPTVRSSAGAIGLLQLLPSTAAEL
DDPAPSEDDLLQPERNAPLGARYLKEMLNAADGNPFLATASYNAGPGAAGSWRNDDLDAIPELWVEAIPYPETRIYVKKV
LGNRWTYALLGDRLTNH

Sequences:

>Translated_577_residues
MQLRGGGVLLISIAAAGTVVISSALQQGLQQIRPELDRHSSPTLLRRTARFDPDPSRRRLARLILANQGDVSASRQQWLL
HAQGWGDNPLSPVVLKQQALAARALGQQERSDRRWTELLQRFPAAAPSADALYYLGQPGDEQHQELLQRFAAHPAALAAA
VDWDRQTQDPRAGLHLAAWGARWPGADAALQRACLRDLGDQQREQVATALAQQGDLDAALNCLGDLRPTAPKTLQWLGLA
AEAPLSPEQQAWEQSRSLLLERDWNGAQEALQQQLEQPLTPPLLARVRFWLGLSAWELGDAEQARQIWQQLLREHPFGYY
GWRASERLQQAPDALPLESPPAEGLLPKDLDQLLALGIPVEAWEQWRMQRGGRPAESPRELWQEGVLRLGVGDRWIALEQ
LDRAQLLGAAEGLSQQVLLEQHRHPRNFEQLLDEAAGNEQLDPDLLLGLARQESRLTPTVRSSAGAIGLLQLLPSTAAEL
DDPAPSEDDLLQPERNAPLGARYLKEMLNAADGNPFLATASYNAGPGAAGSWRNDDLDAIPELWVEAIPYPETRIYVKKV
LGNRWTYALLGDRLTNH
>Mature_577_residues
MQLRGGGVLLISIAAAGTVVISSALQQGLQQIRPELDRHSSPTLLRRTARFDPDPSRRRLARLILANQGDVSASRQQWLL
HAQGWGDNPLSPVVLKQQALAARALGQQERSDRRWTELLQRFPAAAPSADALYYLGQPGDEQHQELLQRFAAHPAALAAA
VDWDRQTQDPRAGLHLAAWGARWPGADAALQRACLRDLGDQQREQVATALAQQGDLDAALNCLGDLRPTAPKTLQWLGLA
AEAPLSPEQQAWEQSRSLLLERDWNGAQEALQQQLEQPLTPPLLARVRFWLGLSAWELGDAEQARQIWQQLLREHPFGYY
GWRASERLQQAPDALPLESPPAEGLLPKDLDQLLALGIPVEAWEQWRMQRGGRPAESPRELWQEGVLRLGVGDRWIALEQ
LDRAQLLGAAEGLSQQVLLEQHRHPRNFEQLLDEAAGNEQLDPDLLLGLARQESRLTPTVRSSAGAIGLLQLLPSTAAEL
DDPAPSEDDLLQPERNAPLGARYLKEMLNAADGNPFLATASYNAGPGAAGSWRNDDLDAIPELWVEAIPYPETRIYVKKV
LGNRWTYALLGDRLTNH

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=164, Percent_Identity=34.1463414634146, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 63902; Mature: 63902

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRGGGVLLISIAAAGTVVISSALQQGLQQIRPELDRHSSPTLLRRTARFDPDPSRRRL
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHH
ARLILANQGDVSASRQQWLLHAQGWGDNPLSPVVLKQQALAARALGQQERSDRRWTELLQ
HHHHHCCCCCCCHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFPAAAPSADALYYLGQPGDEQHQELLQRFAAHPAALAAAVDWDRQTQDPRAGLHLAAWG
HCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEECC
ARWPGADAALQRACLRDLGDQQREQVATALAQQGDLDAALNCLGDLRPTAPKTLQWLGLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHCC
AEAPLSPEQQAWEQSRSLLLERDWNGAQEALQQQLEQPLTPPLLARVRFWLGLSAWELGD
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCC
AEQARQIWQQLLREHPFGYYGWRASERLQQAPDALPLESPPAEGLLPKDLDQLLALGIPV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
EAWEQWRMQRGGRPAESPRELWQEGVLRLGVGDRWIALEQLDRAQLLGAAEGLSQQVLLE
HHHHHHHHHHCCCCCCCHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHRHPRNFEQLLDEAAGNEQLDPDLLLGLARQESRLTPTVRSSAGAIGLLQLLPSTAAEL
HHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHC
DDPAPSEDDLLQPERNAPLGARYLKEMLNAADGNPFLATASYNAGPGAAGSWRNDDLDAI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
PELWVEAIPYPETRIYVKKVLGNRWTYALLGDRLTNH
HHHHHHHCCCCHHHHHHHHHHCCCEEEHHHHHHHCCC
>Mature Secondary Structure
MQLRGGGVLLISIAAAGTVVISSALQQGLQQIRPELDRHSSPTLLRRTARFDPDPSRRRL
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHH
ARLILANQGDVSASRQQWLLHAQGWGDNPLSPVVLKQQALAARALGQQERSDRRWTELLQ
HHHHHCCCCCCCHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFPAAAPSADALYYLGQPGDEQHQELLQRFAAHPAALAAAVDWDRQTQDPRAGLHLAAWG
HCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEECC
ARWPGADAALQRACLRDLGDQQREQVATALAQQGDLDAALNCLGDLRPTAPKTLQWLGLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHCC
AEAPLSPEQQAWEQSRSLLLERDWNGAQEALQQQLEQPLTPPLLARVRFWLGLSAWELGD
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCC
AEQARQIWQQLLREHPFGYYGWRASERLQQAPDALPLESPPAEGLLPKDLDQLLALGIPV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
EAWEQWRMQRGGRPAESPRELWQEGVLRLGVGDRWIALEQLDRAQLLGAAEGLSQQVLLE
HHHHHHHHHHCCCCCCCHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHRHPRNFEQLLDEAAGNEQLDPDLLLGLARQESRLTPTVRSSAGAIGLLQLLPSTAAEL
HHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHC
DDPAPSEDDLLQPERNAPLGARYLKEMLNAADGNPFLATASYNAGPGAAGSWRNDDLDAI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
PELWVEAIPYPETRIYVKKVLGNRWTYALLGDRLTNH
HHHHHHHCCCCHHHHHHHHHHCCCEEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]