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The map label for this gene is bioD [H]

Identifier: 148243026

GI number: 148243026

Start: 1671164

End: 1671844

Strand: Direct

Name: bioD [H]

Synonym: SynRCC307_1927

Alternate gene names: 148243026

Gene position: 1671164-1671844 (Clockwise)

Preceding gene: 148243025

Following gene: 148243035

Centisome position: 75.11

GC content: 64.17

Gene sequence:

>681_bases
ATGAGCCCTAAACCCCGCCATCCCCATCAACTGGTGGTGGTGGGCACCGACACCGACGTGGGCAAGACCGTGATCAGCGC
CCTGTTGGTGCAAGGCCTGGGCGCCCACTACTGGAAGCCGGTGCAGTGCGGTGATCTTGAGATCGGCGGTGATACAGGCC
GTGTCGCCAACCTCTGCGGTTTGAGCGCAGCAGAACAACAGCAGCGGCTGCTCCCCGAGGCCTACCAGCTCAAAGCACCG
GCATCCCCCAACCAAGCCGCTAGGGCTGAGGGCTTTGCCGTTGATCCAGCTCAGTTGAGCTTGCCGCCCGTGGACGGGCC
TTTGGTGGTGGAAACAGCAGGCGGGCTGCTGGTGCCCCTGCGGGATGACTACCTGCAGATTCAACAGATCCAGCAATGGC
AACGTCCGGTCCTGCTGGTGGCCCGCAGCGGACTGGGCACCCTCAACCACACGCTGCTGTCACTGGAAGCCTTGCAGCGC
CGCCAGATCCCGGTGTTGGGGTTGATCCTCAATGGGCCCCGGCACCCAGCGAATCACCAAACCCTTTGCGCCATGAGCGG
CACCACGGTGCTGGCGGAAGTGGAGCAGCAGCCAACACTTGATCAGCAGGCGTTGAGCCGCCTGTGGAGCAGCAGCGGCC
TAGCTGAACGCCTGCCAAAGGCCCTAGAGGCCCGGGCTTGA

Upstream 100 bases:

>100_bases
CCCACAGAACTGCGCCGGCTGCTCAAGCACTGGCCCATGCAGGAGCAGGATCAAGGGGTGAGCCTGAACTGGGATCTCTT
GCTGCTGCTCGCTTGTCGTG

Downstream 100 bases:

>100_bases
CTGCATGATGCCGCCGTCGGCAAAGATGCTGGTGGCGGTGATGTAGCTGGCTCCATCGCTGGCCACAAAGGCAGCCACAG
AGGCGATTTCTTGGGGCTCA

Product: dethiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSPKPRHPHQLVVVGTDTDVGKTVISALLVQGLGAHYWKPVQCGDLEIGGDTGRVANLCGLSAAEQQQRLLPEAYQLKAP
ASPNQAARAEGFAVDPAQLSLPPVDGPLVVETAGGLLVPLRDDYLQIQQIQQWQRPVLLVARSGLGTLNHTLLSLEALQR
RQIPVLGLILNGPRHPANHQTLCAMSGTTVLAEVEQQPTLDQQALSRLWSSSGLAERLPKALEARA

Sequences:

>Translated_226_residues
MSPKPRHPHQLVVVGTDTDVGKTVISALLVQGLGAHYWKPVQCGDLEIGGDTGRVANLCGLSAAEQQQRLLPEAYQLKAP
ASPNQAARAEGFAVDPAQLSLPPVDGPLVVETAGGLLVPLRDDYLQIQQIQQWQRPVLLVARSGLGTLNHTLLSLEALQR
RQIPVLGLILNGPRHPANHQTLCAMSGTTVLAEVEQQPTLDQQALSRLWSSSGLAERLPKALEARA
>Mature_225_residues
SPKPRHPHQLVVVGTDTDVGKTVISALLVQGLGAHYWKPVQCGDLEIGGDTGRVANLCGLSAAEQQQRLLPEAYQLKAPA
SPNQAARAEGFAVDPAQLSLPPVDGPLVVETAGGLLVPLRDDYLQIQQIQQWQRPVLLVARSGLGTLNHTLLSLEALQRR
QIPVLGLILNGPRHPANHQTLCAMSGTTVLAEVEQQPTLDQQALSRLWSSSGLAERLPKALEARA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=202, Percent_Identity=29.7029702970297, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324385, Length=213, Percent_Identity=38.9671361502347, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472 [H]

Pfam domain/function: NA

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 24228; Mature: 24097

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKPRHPHQLVVVGTDTDVGKTVISALLVQGLGAHYWKPVQCGDLEIGGDTGRVANLCG
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHCCCCCCCCCEEECCCCCHHHHHHC
LSAAEQQQRLLPEAYQLKAPASPNQAARAEGFAVDPAQLSLPPVDGPLVVETAGGLLVPL
CCHHHHHHHHCCHHHEECCCCCCCHHHHCCCCEECCHHCCCCCCCCCEEEEECCCEEEEC
RDDYLQIQQIQQWQRPVLLVARSGLGTLNHTLLSLEALQRRQIPVLGLILNGPRHPANHQ
CHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
TLCAMSGTTVLAEVEQQPTLDQQALSRLWSSSGLAERLPKALEARA
EEEEECCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SPKPRHPHQLVVVGTDTDVGKTVISALLVQGLGAHYWKPVQCGDLEIGGDTGRVANLCG
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHCCCCCCCCCEEECCCCCHHHHHHC
LSAAEQQQRLLPEAYQLKAPASPNQAARAEGFAVDPAQLSLPPVDGPLVVETAGGLLVPL
CCHHHHHHHHCCHHHEECCCCCCCHHHHCCCCEECCHHCCCCCCCCCEEEEECCCEEEEC
RDDYLQIQQIQQWQRPVLLVARSGLGTLNHTLLSLEALQRRQIPVLGLILNGPRHPANHQ
CHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
TLCAMSGTTVLAEVEQQPTLDQQALSRLWSSSGLAERLPKALEARA
EEEEECCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]