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The map label for this gene is chaA [H]

Identifier: 148242964

GI number: 148242964

Start: 1623591

End: 1624754

Strand: Direct

Name: chaA [H]

Synonym: SynRCC307_1865

Alternate gene names: 148242964

Gene position: 1623591-1624754 (Clockwise)

Preceding gene: 148242963

Following gene: 148242966

Centisome position: 72.97

GC content: 61.77

Gene sequence:

>1164_bases
GTGGAGGACAACCGTCCTGCTGCGTTGAATCGCCTGCGCCTTCTGCTGCCTGAGCTCGCCACGCTCTCCATGGGAGCGGG
CGTGCTGGCGCTGCTGAGCTTCACCGGGGTCCTGGAATGGCTCCTGGCTGGTAGCGCCAGCACCATCGTGCTGGGCCTGA
TCTTGCTGGGCAGCGTCGTGGTGGTGCTCTCGCTGCAGGTGGCCCGCCAGGCGGAACTGTTAGCGGAAGAGTTGGGTGAG
CCCTTCGGGACGCTGGTGCTCACCGGCTCGGTGATCGTGATCGAGCTGGCCCTAATTGGCAGCACGATGCTGACGGGGGA
ATCGAATCCCACCCTGGCCCGGGATTCAATGTTCTCGGTCTTGATGGTGGCCCTCACCGGCATCACCGGGTTGTGCATGG
CCCTCACCTCCCGCATGCAGAAAAAAGACCGTGATCAGCCGATGCGGGTCGAAGACCTGGCGGCCACGAACAAGGCGGGG
GCGATGGTCTACTACAACCTCATCGGCACGATGAGCGTGCTGGTGCTGATCATTCCCAACTTCAGCAACGACAGCCCTGA
AGGCAATTTCAGCCTGCCGCTGAACATCGTTCTCTCCGTCACCGCACTGGTGGTCTACGGCCTGTTCCTGTTCAACCAAA
CCGGTGCTTACCGCGGCTTCTTCATGGAGATGTTTGCCGACGAGGGCGGCAATCCAGAAAGCCGCCATCACGCCGAACTC
GAAGGGGGCCCCTGGCAAGCCGCCGCCCTGCTGATGGGAACCCTGGCGATCGTGGTGGTGATCGCTGAATCCATGGGCCA
ACTCATTGAGCGCGGAGTCAATGAGCTGCACTTGCCCAGCTCCCTGGCTGGCATCCTCGTGGCGATGCTGATCCTGCTGC
CGGAGGCCATCAATGCGATCCAAGCAACGCGGAAAGGCAAGTTGCAGCGGGCCCTCAACACCCTCTATGGCTCGGTGCTC
TCCACCATCAGCCTGACGGTTCCTGCGGTGCTGCTCATCGGAGAAGTGACTGGCACCAAGGTGATCTTGGGGCTGGAGCA
ATACGAAATGGTGCTCCTCTCGCTCACCTTGTTTCTGCTGCGCCCCCACAGCCGCGTGCTGGGAAGCGAAGGGCTGATGT
TGCTCGTGGTGTTTCTGTTCTGGTTCGTGCTGGAGCTGGTCTGA

Upstream 100 bases:

>100_bases
GCAACTACTGCAGGCCGGTGCCGATCACATCCTGGGCAGCACTCCAGAGCTGCTTTTTTTCATTGATCAGCTGACAATCC
CTCAACACTGAGTTCAAGGT

Downstream 100 bases:

>100_bases
ACTCAGCGCTCCATCGCTTCCACACTCTTGAGGGCGGCGGCGGTGAGCACCTCAGCGCCCCCATCGACCACCGCCACATC
GTCTTCGATGCGAATCCCGA

Product: calcium/proton antiporter

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEDNRPAALNRLRLLLPELATLSMGAGVLALLSFTGVLEWLLAGSASTIVLGLILLGSVVVVLSLQVARQAELLAEELGE
PFGTLVLTGSVIVIELALIGSTMLTGESNPTLARDSMFSVLMVALTGITGLCMALTSRMQKKDRDQPMRVEDLAATNKAG
AMVYYNLIGTMSVLVLIIPNFSNDSPEGNFSLPLNIVLSVTALVVYGLFLFNQTGAYRGFFMEMFADEGGNPESRHHAEL
EGGPWQAAALLMGTLAIVVVIAESMGQLIERGVNELHLPSSLAGILVAMLILLPEAINAIQATRKGKLQRALNTLYGSVL
STISLTVPAVLLIGEVTGTKVILGLEQYEMVLLSLTLFLLRPHSRVLGSEGLMLLVVFLFWFVLELV

Sequences:

>Translated_387_residues
MEDNRPAALNRLRLLLPELATLSMGAGVLALLSFTGVLEWLLAGSASTIVLGLILLGSVVVVLSLQVARQAELLAEELGE
PFGTLVLTGSVIVIELALIGSTMLTGESNPTLARDSMFSVLMVALTGITGLCMALTSRMQKKDRDQPMRVEDLAATNKAG
AMVYYNLIGTMSVLVLIIPNFSNDSPEGNFSLPLNIVLSVTALVVYGLFLFNQTGAYRGFFMEMFADEGGNPESRHHAEL
EGGPWQAAALLMGTLAIVVVIAESMGQLIERGVNELHLPSSLAGILVAMLILLPEAINAIQATRKGKLQRALNTLYGSVL
STISLTVPAVLLIGEVTGTKVILGLEQYEMVLLSLTLFLLRPHSRVLGSEGLMLLVVFLFWFVLELV
>Mature_387_residues
MEDNRPAALNRLRLLLPELATLSMGAGVLALLSFTGVLEWLLAGSASTIVLGLILLGSVVVVLSLQVARQAELLAEELGE
PFGTLVLTGSVIVIELALIGSTMLTGESNPTLARDSMFSVLMVALTGITGLCMALTSRMQKKDRDQPMRVEDLAATNKAG
AMVYYNLIGTMSVLVLIIPNFSNDSPEGNFSLPLNIVLSVTALVVYGLFLFNQTGAYRGFFMEMFADEGGNPESRHHAEL
EGGPWQAAALLMGTLAIVVVIAESMGQLIERGVNELHLPSSLAGILVAMLILLPEAINAIQATRKGKLQRALNTLYGSVL
STISLTVPAVLLIGEVTGTKVILGLEQYEMVLLSLTLFLLRPHSRVLGSEGLMLLVVFLFWFVLELV

Specific function: According to PubMed:8496184 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=305, Percent_Identity=32.7868852459016, Blast_Score=151, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 41447; Mature: 41447

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDNRPAALNRLRLLLPELATLSMGAGVLALLSFTGVLEWLLAGSASTIVLGLILLGSVV
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VVLSLQVARQAELLAEELGEPFGTLVLTGSVIVIELALIGSTMLTGESNPTLARDSMFSV
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LMVALTGITGLCMALTSRMQKKDRDQPMRVEDLAATNKAGAMVYYNLIGTMSVLVLIIPN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
FSNDSPEGNFSLPLNIVLSVTALVVYGLFLFNQTGAYRGFFMEMFADEGGNPESRHHAEL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
EGGPWQAAALLMGTLAIVVVIAESMGQLIERGVNELHLPSSLAGILVAMLILLPEAINAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QATRKGKLQRALNTLYGSVLSTISLTVPAVLLIGEVTGTKVILGLEQYEMVLLSLTLFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHH
RPHSRVLGSEGLMLLVVFLFWFVLELV
CCHHHHHCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MEDNRPAALNRLRLLLPELATLSMGAGVLALLSFTGVLEWLLAGSASTIVLGLILLGSVV
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VVLSLQVARQAELLAEELGEPFGTLVLTGSVIVIELALIGSTMLTGESNPTLARDSMFSV
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LMVALTGITGLCMALTSRMQKKDRDQPMRVEDLAATNKAGAMVYYNLIGTMSVLVLIIPN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
FSNDSPEGNFSLPLNIVLSVTALVVYGLFLFNQTGAYRGFFMEMFADEGGNPESRHHAEL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
EGGPWQAAALLMGTLAIVVVIAESMGQLIERGVNELHLPSSLAGILVAMLILLPEAINAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QATRKGKLQRALNTLYGSVLSTISLTVPAVLLIGEVTGTKVILGLEQYEMVLLSLTLFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHH
RPHSRVLGSEGLMLLVVFLFWFVLELV
CCHHHHHCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8496184; 8905232; 9278503 [H]