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The map label for this gene is metG [H]

Identifier: 148242327

GI number: 148242327

Start: 1089122

End: 1090654

Strand: Direct

Name: metG [H]

Synonym: SynRCC307_1228

Alternate gene names: 148242327

Gene position: 1089122-1090654 (Clockwise)

Preceding gene: 148242325

Following gene: 148242328

Centisome position: 48.95

GC content: 55.51

Gene sequence:

>1533_bases
ATGACAAGCACACTCACCACTCCGCTTTATTACGTCAACGACAAGCCCCACATCGGCAGTGTTTACACCACGTTGGCAGT
CGATACTCTGGCCCGTTTTAAGCGACTTAGCGGCGAGAATGTGGTGTTCATCACGGGATGCGATGAACACGGTCAAAAGA
TTCAACGAACCGCTGAATCAGCGGGGATTGATCCTCAACAACATTGCGACCAAATCAGCAGCACCTTTGCATCGGTGTGG
TCGCAGTGGCAGATCAGCAATGATCGTTTCATACGCACCACAGATCCCCGCCACCAATCGATTGTTGAGCAGTTCTTCGC
CCGAGTCGAAGCCAACGGCGACATTATTGAAGGCCGGCAACAAGGCTGGTATTGCGTTGCCTGTGAAGAATACAAAGATG
ATCCGAAAGAGGCCCAATCACCCCACTGCCCGATTCACCAGAAACCCTTGGAATGGCGTGATGAGGCCAACCTGTTCTTC
CGGCTGAGTCGTTACCAAGACGCCATTGAAAAGCTCATTGCAACCGAGGGCTTCATTCGCCCAGCCAGCCGGCGCAAAGA
AGTTGAAAATTTTGTTGCCAACGGCCTTCGAGATTTCTCCATTTCCCGGCGAAACGTGAGCTGGGGTCTGCCGGTCCCTG
GTCATGACGGGCACACCTTCTACGTCTGGTTTGACGCCCTGTTGGGCTACATCACAGCCCTTCTAGAGCCTGATCAGCCA
GCCACGCTGGATCAAGCCCTCAGTAATGGCTGGCCAGCCCAATTGCACGTGATTGGCAAAGACATCTTGCGCTTCCACGC
TGTGTATTGGCCCGCGATGTGTTTATCCGCTGGGATTGATTTGCCAACAGGGGTGTTTGGCCATGGCTTCCTCACCCGGG
AAGGTCAAAAAATGGGCAAGTCCATGGGGAATTCCATTGACCCCACCCGTTTGGTTGAGCTCTGCGGCCGCGATGCTGTG
CGCTGGTACCTGCTGCGTGACATCCAATTTGGCGACGATGGCGATATCCAGCAGCAGCGCCTCTTGGATCTGGTCAACAA
CGATCTAGCCAACACCATCGGCAATCTGGTGAATCGCTCCATTTCGATGGCCCGTAAATGGTTTGAGGGTGTTCCATCAC
TGCCTCAAGAGCTCCCCAGCAACCATCCCCTCAAGCAAGCCGCACAAGCCGCGGTGGCTGATGTGGCGAGCCACTACCAA
GAGCTCAGGTTCCATGCTGTGGCCGAACAGGCGCTGCAGTTGGCCATTGCCGCCAACGGTTACCTCAGTGATCAAGCTCC
TTGGAAGGCCATCAAAGATCCAGCCAACCGCGATCAAGTGGCCGCTGACTTGTATGCGGTGCTGGAAGCCAGCCGCGTGG
TGGGTGTTGTGCTGCAACCGCTCTTGCCCGAGTTCAGTGAACGGCTTTTGCAGCAGCTCAATGCACCCTCAGGGGATTGG
ATCGAGCTTTTGAATTGGGGGCAGCTCACGCCAGGCCCATTGGATGCACCATCCCCGCTGCTGCAACGCTTGGAGCTGGA
GGAGCCAATTTGA

Upstream 100 bases:

>100_bases
ACCTATGACCAGAACGGGGACGTGGCCCTGCTGAAAAGCCATCGCTGCACTGAACTGAGATCATCATGCTGACGGGCGCA
GCCTCAGTAAGATCGCGTTG

Downstream 100 bases:

>100_bases
AGGTCTTTGCTCTAGGCGCGGGAGCGGGGCTGCTCTGTTTGCTGTTGCATGGATGCGTCGGTGTGTCGCAAAGTCCAGAG
CCAGCGAAACCGTTTCAGTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTSTLTTPLYYVNDKPHIGSVYTTLAVDTLARFKRLSGENVVFITGCDEHGQKIQRTAESAGIDPQQHCDQISSTFASVW
SQWQISNDRFIRTTDPRHQSIVEQFFARVEANGDIIEGRQQGWYCVACEEYKDDPKEAQSPHCPIHQKPLEWRDEANLFF
RLSRYQDAIEKLIATEGFIRPASRRKEVENFVANGLRDFSISRRNVSWGLPVPGHDGHTFYVWFDALLGYITALLEPDQP
ATLDQALSNGWPAQLHVIGKDILRFHAVYWPAMCLSAGIDLPTGVFGHGFLTREGQKMGKSMGNSIDPTRLVELCGRDAV
RWYLLRDIQFGDDGDIQQQRLLDLVNNDLANTIGNLVNRSISMARKWFEGVPSLPQELPSNHPLKQAAQAAVADVASHYQ
ELRFHAVAEQALQLAIAANGYLSDQAPWKAIKDPANRDQVAADLYAVLEASRVVGVVLQPLLPEFSERLLQQLNAPSGDW
IELLNWGQLTPGPLDAPSPLLQRLELEEPI

Sequences:

>Translated_510_residues
MTSTLTTPLYYVNDKPHIGSVYTTLAVDTLARFKRLSGENVVFITGCDEHGQKIQRTAESAGIDPQQHCDQISSTFASVW
SQWQISNDRFIRTTDPRHQSIVEQFFARVEANGDIIEGRQQGWYCVACEEYKDDPKEAQSPHCPIHQKPLEWRDEANLFF
RLSRYQDAIEKLIATEGFIRPASRRKEVENFVANGLRDFSISRRNVSWGLPVPGHDGHTFYVWFDALLGYITALLEPDQP
ATLDQALSNGWPAQLHVIGKDILRFHAVYWPAMCLSAGIDLPTGVFGHGFLTREGQKMGKSMGNSIDPTRLVELCGRDAV
RWYLLRDIQFGDDGDIQQQRLLDLVNNDLANTIGNLVNRSISMARKWFEGVPSLPQELPSNHPLKQAAQAAVADVASHYQ
ELRFHAVAEQALQLAIAANGYLSDQAPWKAIKDPANRDQVAADLYAVLEASRVVGVVLQPLLPEFSERLLQQLNAPSGDW
IELLNWGQLTPGPLDAPSPLLQRLELEEPI
>Mature_509_residues
TSTLTTPLYYVNDKPHIGSVYTTLAVDTLARFKRLSGENVVFITGCDEHGQKIQRTAESAGIDPQQHCDQISSTFASVWS
QWQISNDRFIRTTDPRHQSIVEQFFARVEANGDIIEGRQQGWYCVACEEYKDDPKEAQSPHCPIHQKPLEWRDEANLFFR
LSRYQDAIEKLIATEGFIRPASRRKEVENFVANGLRDFSISRRNVSWGLPVPGHDGHTFYVWFDALLGYITALLEPDQPA
TLDQALSNGWPAQLHVIGKDILRFHAVYWPAMCLSAGIDLPTGVFGHGFLTREGQKMGKSMGNSIDPTRLVELCGRDAVR
WYLLRDIQFGDDGDIQQQRLLDLVNNDLANTIGNLVNRSISMARKWFEGVPSLPQELPSNHPLKQAAQAAVADVASHYQE
LRFHAVAEQALQLAIAANGYLSDQAPWKAIKDPANRDQVAADLYAVLEASRVVGVVLQPLLPEFSERLLQQLNAPSGDWI
ELLNWGQLTPGPLDAPSPLLQRLELEEPI

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=505, Percent_Identity=34.6534653465347, Blast_Score=299, Evalue=5e-81,
Organism=Homo sapiens, GI14043022, Length=554, Percent_Identity=26.3537906137184, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1788432, Length=513, Percent_Identity=27.4853801169591, Blast_Score=188, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI212645096, Length=521, Percent_Identity=30.3262955854127, Blast_Score=261, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI71992781, Length=413, Percent_Identity=27.8450363196126, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17540794, Length=549, Percent_Identity=23.6794171220401, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321610, Length=523, Percent_Identity=31.1663479923518, Blast_Score=247, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321704, Length=546, Percent_Identity=23.992673992674, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI21358483, Length=524, Percent_Identity=33.3969465648855, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24655311, Length=542, Percent_Identity=23.8007380073801, Blast_Score=143, Evalue=4e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57241; Mature: 57110

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTLTTPLYYVNDKPHIGSVYTTLAVDTLARFKRLSGENVVFITGCDEHGQKIQRTAES
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
AGIDPQQHCDQISSTFASVWSQWQISNDRFIRTTDPRHQSIVEQFFARVEANGDIIEGRQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEECCCC
QGWYCVACEEYKDDPKEAQSPHCPIHQKPLEWRDEANLFFRLSRYQDAIEKLIATEGFIR
CCEEEEEEHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
PASRRKEVENFVANGLRDFSISRRNVSWGLPVPGHDGHTFYVWFDALLGYITALLEPDQP
CHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCC
ATLDQALSNGWPAQLHVIGKDILRFHAVYWPAMCLSAGIDLPTGVFGHGFLTREGQKMGK
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
SMGNSIDPTRLVELCGRDAVRWYLLRDIQFGDDGDIQQQRLLDLVNNDLANTIGNLVNRS
HHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ISMARKWFEGVPSLPQELPSNHPLKQAAQAAVADVASHYQELRFHAVAEQALQLAIAANG
HHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YLSDQAPWKAIKDPANRDQVAADLYAVLEASRVVGVVLQPLLPEFSERLLQQLNAPSGDW
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
IELLNWGQLTPGPLDAPSPLLQRLELEEPI
HHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TSTLTTPLYYVNDKPHIGSVYTTLAVDTLARFKRLSGENVVFITGCDEHGQKIQRTAES
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
AGIDPQQHCDQISSTFASVWSQWQISNDRFIRTTDPRHQSIVEQFFARVEANGDIIEGRQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEECCCC
QGWYCVACEEYKDDPKEAQSPHCPIHQKPLEWRDEANLFFRLSRYQDAIEKLIATEGFIR
CCEEEEEEHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
PASRRKEVENFVANGLRDFSISRRNVSWGLPVPGHDGHTFYVWFDALLGYITALLEPDQP
CHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCC
ATLDQALSNGWPAQLHVIGKDILRFHAVYWPAMCLSAGIDLPTGVFGHGFLTREGQKMGK
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
SMGNSIDPTRLVELCGRDAVRWYLLRDIQFGDDGDIQQQRLLDLVNNDLANTIGNLVNRS
HHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ISMARKWFEGVPSLPQELPSNHPLKQAAQAAVADVASHYQELRFHAVAEQALQLAIAANG
HHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YLSDQAPWKAIKDPANRDQVAADLYAVLEASRVVGVVLQPLLPEFSERLLQQLNAPSGDW
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
IELLNWGQLTPGPLDAPSPLLQRLELEEPI
HHHHHCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]