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The map label for this gene is ilvE [H]

Identifier: 148242307

GI number: 148242307

Start: 1067088

End: 1068002

Strand: Direct

Name: ilvE [H]

Synonym: SynRCC307_1208

Alternate gene names: 148242307

Gene position: 1067088-1068002 (Clockwise)

Preceding gene: 148242305

Following gene: 148242308

Centisome position: 47.96

GC content: 62.4

Gene sequence:

>915_bases
ATGCATCGCTTCCTGCCCTACGCCTGGTTCGGCGGACGCTGCGTGCCATTTGCTGAAGCCACGCTGTCGGTGGCGACCCA
TGCCTTGCACTACGGCACCGGTGCCTTTGGCGGCATGCGGGCCCTGCCAAATCCTCAAGATCCCGGCGAGATCCTGCTGT
TCCGGGCTGATCGCCATGCCAAGCGCCTGAGCCAGAGCGCACGCTTGCTGCTCACTGATTTGCCCGCGTCCACTGTTTTG
GAGGCGGTGGAGGCTTTCCTGAAGGCCAACCAGCCCAGCTGTCCGATCTACCTGCGCCCATTCGTTTACACCTCCGATCT
CGGTATCGCCCCGCGGCTGCACGACATCGAGACCGATTTTCTGATCTATGGCCTGGAACTGGGGGACTACCTCTCTCCGG
AGGGCGTCAGCTGCCGGATCAGCTCCTGGACACGCCAGGAGGACCGCTCTCTCCCCCTGAGGGGCAAGATCAGTGGGGCC
TACATCACCAGCTCCCTGGCCAAGACAGAGGCAGTGAAGAGCGGATTTGATGAGGCGCTGCTTCTGAACAGCCGCGGCAA
GGTGAGCGAAGCCAGCGGCATGAACCTGTTCATCGTTCGCGATGGGGTGCTCTACACCCCTGGCGTCGACCAGGACATCC
TGGAGGGAATCACCCGTGGCAGCGTGATCGAGCTGGCTCGCCACATGGGCTACACCGTGGTGGAACGGCCGATCGACAAG
AGCGAATTATTTGTTGCCGAGGAGGTTTTCCTGACCGGCACCGCCGCCAAGGTCACCCCGGTGCGGCGCGTGGAATGCAC
CGACCTGTCCTCTGATCGGCCGGTCATGGATGCCTTGCGCGAGCGCATCACCGCCATTACCGAGGGGCGAGACCCTAACT
TTGAGCACTGGGTGACGCGGATTCGGATTAAGTGA

Upstream 100 bases:

>100_bases
CCCCTCGCCGGGATCCCCTGGCAGTGCTGCCAATCTGTGCATGGCTTGATTCTCGCTCAGCAGAGGAGGTGAGAAGATCT
GCCCAGGTCTGTGTCGCTCC

Downstream 100 bases:

>100_bases
TGCACCGCTTTCTGGAGAGACTGCACGCTCCGGAGCGGCCAGTTCTCGTCTTCGATGGGGCGACGGGTACCTCGCTCCAA
CAGATGGATCTTGGCCCCGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MHRFLPYAWFGGRCVPFAEATLSVATHALHYGTGAFGGMRALPNPQDPGEILLFRADRHAKRLSQSARLLLTDLPASTVL
EAVEAFLKANQPSCPIYLRPFVYTSDLGIAPRLHDIETDFLIYGLELGDYLSPEGVSCRISSWTRQEDRSLPLRGKISGA
YITSSLAKTEAVKSGFDEALLLNSRGKVSEASGMNLFIVRDGVLYTPGVDQDILEGITRGSVIELARHMGYTVVERPIDK
SELFVAEEVFLTGTAAKVTPVRRVECTDLSSDRPVMDALRERITAITEGRDPNFEHWVTRIRIK

Sequences:

>Translated_304_residues
MHRFLPYAWFGGRCVPFAEATLSVATHALHYGTGAFGGMRALPNPQDPGEILLFRADRHAKRLSQSARLLLTDLPASTVL
EAVEAFLKANQPSCPIYLRPFVYTSDLGIAPRLHDIETDFLIYGLELGDYLSPEGVSCRISSWTRQEDRSLPLRGKISGA
YITSSLAKTEAVKSGFDEALLLNSRGKVSEASGMNLFIVRDGVLYTPGVDQDILEGITRGSVIELARHMGYTVVERPIDK
SELFVAEEVFLTGTAAKVTPVRRVECTDLSSDRPVMDALRERITAITEGRDPNFEHWVTRIRIK
>Mature_304_residues
MHRFLPYAWFGGRCVPFAEATLSVATHALHYGTGAFGGMRALPNPQDPGEILLFRADRHAKRLSQSARLLLTDLPASTVL
EAVEAFLKANQPSCPIYLRPFVYTSDLGIAPRLHDIETDFLIYGLELGDYLSPEGVSCRISSWTRQEDRSLPLRGKISGA
YITSSLAKTEAVKSGFDEALLLNSRGKVSEASGMNLFIVRDGVLYTPGVDQDILEGITRGSVIELARHMGYTVVERPIDK
SELFVAEEVFLTGTAAKVTPVRRVECTDLSSDRPVMDALRERITAITEGRDPNFEHWVTRIRIK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=319, Percent_Identity=27.8996865203762, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI38176287, Length=319, Percent_Identity=27.8996865203762, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI296010904, Length=319, Percent_Identity=27.8996865203762, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI48994963, Length=305, Percent_Identity=33.1147540983607, Blast_Score=180, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6322002, Length=327, Percent_Identity=23.8532110091743, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322608, Length=284, Percent_Identity=23.943661971831, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33683; Mature: 33683

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00037 MYB_1 ; PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRFLPYAWFGGRCVPFAEATLSVATHALHYGTGAFGGMRALPNPQDPGEILLFRADRHA
CCCCCCEEEECCEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHH
KRLSQSARLLLTDLPASTVLEAVEAFLKANQPSCPIYLRPFVYTSDLGIAPRLHDIETDF
HHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEECEEEECCCCCCCCCCCCCCCE
LIYGLELGDYLSPEGVSCRISSWTRQEDRSLPLRGKISGAYITSSLAKTEAVKSGFDEAL
EEEEEECCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
LLNSRGKVSEASGMNLFIVRDGVLYTPGVDQDILEGITRGSVIELARHMGYTVVERPIDK
EECCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHCCCCHHHHHHHHCCCEEECCCCCH
SELFVAEEVFLTGTAAKVTPVRRVECTDLSSDRPVMDALRERITAITEGRDPNFEHWVTR
HHEEEEEHHEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEEE
IRIK
EECC
>Mature Secondary Structure
MHRFLPYAWFGGRCVPFAEATLSVATHALHYGTGAFGGMRALPNPQDPGEILLFRADRHA
CCCCCCEEEECCEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHH
KRLSQSARLLLTDLPASTVLEAVEAFLKANQPSCPIYLRPFVYTSDLGIAPRLHDIETDF
HHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEECEEEECCCCCCCCCCCCCCCE
LIYGLELGDYLSPEGVSCRISSWTRQEDRSLPLRGKISGAYITSSLAKTEAVKSGFDEAL
EEEEEECCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
LLNSRGKVSEASGMNLFIVRDGVLYTPGVDQDILEGITRGSVIELARHMGYTVVERPIDK
EECCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHCCCCHHHHHHHHCCCEEECCCCCH
SELFVAEEVFLTGTAAKVTPVRRVECTDLSSDRPVMDALRERITAITEGRDPNFEHWVTR
HHEEEEEHHEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEEE
IRIK
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]