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The map label for this gene is nifS [H]

Identifier: 148242290

GI number: 148242290

Start: 1049699

End: 1050946

Strand: Direct

Name: nifS [H]

Synonym: SynRCC307_1191

Alternate gene names: 148242290

Gene position: 1049699-1050946 (Clockwise)

Preceding gene: 148242289

Following gene: 148242291

Centisome position: 47.18

GC content: 62.66

Gene sequence:

>1248_bases
TTGATCGTCTGTCGCTTGATGACATGGTGGGTTTGGCGAATTCATCGCTTGAAGAGCGCACCCGCCGTCGTGCCGGCTTC
TAGGCCTCTGATCTATCTGGATGCCTGCGCTGCAACGCCCCTCGCTGAGGGGCTTGGGCAACGGCTGCTGGATTACCAAC
AGCAGGCTTGGGCTAATCCCTCAAGCCTTCATCCGGCAGGGCTTGCTGCTGCCGAGTTACTGGAACGCAGCCGTCAGAGG
CTGTTGGAGCTATTGGACTGCCCCAATGGCCAGGTCGTCTTCACATCAGGCGGGACAGAAGCAGACAACTTGGCCCTGTT
CGGGGTCTGCCGCAGGCAAACACCGGGCCGTCTGTTGATTTCAGCCTTTGAGCATGCGGCCATTGGCCGCAGCGCCGATC
AGTTGCGCGCCGAGGGCTGGCAAGTGGAGATCCTGCCCCTGGATCACCACGGGCGGATCGACCTTCAGGCCCTCGAAGGA
TTGCTGGTTCCACCGACTCGCCTTGTCAGCGTGGCGTGGGCCAGCTCAGAGCTCGGTGTTCTGCAGCCCATGGAAGCGAT
CAGCCGCCTGTGCCATCGAGCTGGTGTGCCATTGCACAGCGATGCGGTGCAGGTGGTCGGTCAACTCCCCGTTGCGCAGT
TGCTGCCGTTGCCGGTGGATCTGCTGAGCATCAGCGCCCACAAATTTCAGGGCCCCAGGGGAGTGGGCGCCCTGGTGATG
CCCAAGCCGCAGGCTCTGCAACCTCAGTTGATTGGCGGCGGGCAGGAATCAGGACTGCGATCTGGAACCGAATCACCTTG
GTTGGTGGCGGCCATGGTGGATGCCCTGGAATGTCGCTTCAACCTGCAACCCCAGCTGGCTGAAGCGATGGCTGACCTGC
GGGATGAGCTTTGGCAGCGACTAGCCGCCGTTGCTGGCCTTGAGCGGGTTGGCCGCTGGAGGGGCTCGGAGTGTTTGCCA
CACCATTTGGCTGTGCTGCTGTCGTCAGTGGAGGGAGAGCCGCTCTCAGCACGCGCCGCCGTGCGCCAACTGGCCCAAGC
CGGTGTGGCGGTGAGCAGTGGTTCGGCTTGCAGCAGCGGCACCGATCAAGCCAACCCGGCGCTGTTGGCATTGGGTTACA
ACCCCAGGCAGGCCCGTAGTGGATTGCGCTTCAGTGTGGGGCCCTGGCTGCAGCCACAGGACTTCATTGGCTTACCGCCT
CTGCTGGAATCGGTACTGCGCCAATTGCCCACCGTTGAGTTGCCATGA

Upstream 100 bases:

>100_bases
GCCGTTGAGGCCATTGACTGCGCCAGCCTCTGCAGCGACGGCTGCATCCGGCCTGAGGCCTGCCCCAGTGCTGCGGCACG
GGAACAAGCGATGACATTCC

Downstream 100 bases:

>100_bases
GCCTTCCTGCTGATCTCGTTGCCGCTGAGGCCGAGTTGCTGACAGCGCTGGAGTCGGCCCTGGCCAGCAATGCAAAAGGC
CGCTGGACCTTGGATCTGCG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MIVCRLMTWWVWRIHRLKSAPAVVPASRPLIYLDACAATPLAEGLGQRLLDYQQQAWANPSSLHPAGLAAAELLERSRQR
LLELLDCPNGQVVFTSGGTEADNLALFGVCRRQTPGRLLISAFEHAAIGRSADQLRAEGWQVEILPLDHHGRIDLQALEG
LLVPPTRLVSVAWASSELGVLQPMEAISRLCHRAGVPLHSDAVQVVGQLPVAQLLPLPVDLLSISAHKFQGPRGVGALVM
PKPQALQPQLIGGGQESGLRSGTESPWLVAAMVDALECRFNLQPQLAEAMADLRDELWQRLAAVAGLERVGRWRGSECLP
HHLAVLLSSVEGEPLSARAAVRQLAQAGVAVSSGSACSSGTDQANPALLALGYNPRQARSGLRFSVGPWLQPQDFIGLPP
LLESVLRQLPTVELP

Sequences:

>Translated_415_residues
MIVCRLMTWWVWRIHRLKSAPAVVPASRPLIYLDACAATPLAEGLGQRLLDYQQQAWANPSSLHPAGLAAAELLERSRQR
LLELLDCPNGQVVFTSGGTEADNLALFGVCRRQTPGRLLISAFEHAAIGRSADQLRAEGWQVEILPLDHHGRIDLQALEG
LLVPPTRLVSVAWASSELGVLQPMEAISRLCHRAGVPLHSDAVQVVGQLPVAQLLPLPVDLLSISAHKFQGPRGVGALVM
PKPQALQPQLIGGGQESGLRSGTESPWLVAAMVDALECRFNLQPQLAEAMADLRDELWQRLAAVAGLERVGRWRGSECLP
HHLAVLLSSVEGEPLSARAAVRQLAQAGVAVSSGSACSSGTDQANPALLALGYNPRQARSGLRFSVGPWLQPQDFIGLPP
LLESVLRQLPTVELP
>Mature_415_residues
MIVCRLMTWWVWRIHRLKSAPAVVPASRPLIYLDACAATPLAEGLGQRLLDYQQQAWANPSSLHPAGLAAAELLERSRQR
LLELLDCPNGQVVFTSGGTEADNLALFGVCRRQTPGRLLISAFEHAAIGRSADQLRAEGWQVEILPLDHHGRIDLQALEG
LLVPPTRLVSVAWASSELGVLQPMEAISRLCHRAGVPLHSDAVQVVGQLPVAQLLPLPVDLLSISAHKFQGPRGVGALVM
PKPQALQPQLIGGGQESGLRSGTESPWLVAAMVDALECRFNLQPQLAEAMADLRDELWQRLAAVAGLERVGRWRGSECLP
HHLAVLLSSVEGEPLSARAAVRQLAQAGVAVSSGSACSSGTDQANPALLALGYNPRQARSGLRFSVGPWLQPQDFIGLPP
LLESVLRQLPTVELP

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=384, Percent_Identity=35.15625, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI156713448, Length=396, Percent_Identity=31.8181818181818, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI48994898, Length=372, Percent_Identity=35.4838709677419, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787970, Length=252, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789175, Length=404, Percent_Identity=26.4851485148515, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25143064, Length=390, Percent_Identity=32.8205128205128, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17533177, Length=314, Percent_Identity=29.6178343949045, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319831, Length=376, Percent_Identity=34.5744680851064, Blast_Score=190, Evalue=4e-49,
Organism=Drosophila melanogaster, GI20129463, Length=375, Percent_Identity=35.7333333333333, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44656; Mature: 44656

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVCRLMTWWVWRIHRLKSAPAVVPASRPLIYLDACAATPLAEGLGQRLLDYQQQAWANP
CEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHCCHHHHHHHHHHHHHHHHHCCCC
SSLHPAGLAAAELLERSRQRLLELLDCPNGQVVFTSGGTEADNLALFGVCRRQTPGRLLI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEHHCCCCCHHHH
SAFEHAAIGRSADQLRAEGWQVEILPLDHHGRIDLQALEGLLVPPTRLVSVAWASSELGV
HHHHHHHHCCCHHHHHHCCCEEEEEEECCCCCEEHHHHCCCCCCCHHHHEEEECCCCCCC
LQPMEAISRLCHRAGVPLHSDAVQVVGQLPVAQLLPLPVDLLSISAHKFQGPRGVGALVM
CCHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCCCCEEEC
PKPQALQPQLIGGGQESGLRSGTESPWLVAAMVDALECRFNLQPQLAEAMADLRDELWQR
CCCCCCCCHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LAAVAGLERVGRWRGSECLPHHLAVLLSSVEGEPLSARAAVRQLAQAGVAVSSGSACSSG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCCCCC
TDQANPALLALGYNPRQARSGLRFSVGPWLQPQDFIGLPPLLESVLRQLPTVELP
CCCCCCEEEEECCCHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIVCRLMTWWVWRIHRLKSAPAVVPASRPLIYLDACAATPLAEGLGQRLLDYQQQAWANP
CEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHCCHHHHHHHHHHHHHHHHHCCCC
SSLHPAGLAAAELLERSRQRLLELLDCPNGQVVFTSGGTEADNLALFGVCRRQTPGRLLI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEHHCCCCCHHHH
SAFEHAAIGRSADQLRAEGWQVEILPLDHHGRIDLQALEGLLVPPTRLVSVAWASSELGV
HHHHHHHHCCCHHHHHHCCCEEEEEEECCCCCEEHHHHCCCCCCCHHHHEEEECCCCCCC
LQPMEAISRLCHRAGVPLHSDAVQVVGQLPVAQLLPLPVDLLSISAHKFQGPRGVGALVM
CCHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCCCCEEEC
PKPQALQPQLIGGGQESGLRSGTESPWLVAAMVDALECRFNLQPQLAEAMADLRDELWQR
CCCCCCCCHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LAAVAGLERVGRWRGSECLPHHLAVLLSSVEGEPLSARAAVRQLAQAGVAVSSGSACSSG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCCCCC
TDQANPALLALGYNPRQARSGLRFSVGPWLQPQDFIGLPPLLESVLRQLPTVELP
CCCCCCEEEEECCCHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA