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The map label for this gene is rpoD [H]

Identifier: 148241988

GI number: 148241988

Start: 806376

End: 807581

Strand: Direct

Name: rpoD [H]

Synonym: SynRCC307_0889

Alternate gene names: 148241988

Gene position: 806376-807581 (Clockwise)

Preceding gene: 148241986

Following gene: 148241991

Centisome position: 36.24

GC content: 60.36

Gene sequence:

>1206_bases
ATGCCCGTAGCTGCTGCCGCTAAGTCCACAACCGCTGTCGCGGAAGCCACCGAAGCCAAGCCGGTGAAAGCCAAGGCTGC
TCCCAAAGCCAAGGCAGCCCCTAAGGCCAAGGCTGCTCCCAAAGCCAAGGCAGCCCCTAAGGCCAAGGCTGCTCCGAAGG
CCAAAGCCGAGCCTCAGGCTGAAGTTGCTGTCACGACCACCGTCGCGAAAGACGGCAAGGCCGCCAAGCTGCCCAGCATC
AAGATCGGCCCGAAGGGCGTCTACACAGAAGACTCCATCCGGGTGTATCTGCAGGAGATCGGCCGCATCCGCCTGCTGCG
CCCTGATGAAGAGATCGAGCTGGCCCGCAAGATCGCCGACCTGCTTCACCTCGAAGAGCTGGCTGATCAGTACGAGGAAG
ACCACGGCAAATATCCCGACAACCGCGAGTGGGCTGTCCTGGTCGACATGCCCCCCATCAAGTTCCGTCGCCGCTTGATG
CTCGGCCGCCGGGCCAAAGAAAAGATGGTGCAGTCCAACCTGCGCCTGGTTGTGTCGATCGCCAAGAAGTACATGAACCG
GGGCCTGAGCTTCCAGGACCTGATCCAAGAAGGCAGCCTTGGCTTGATCCGCGCGGCTGAGAAGTTCGACCACGAGAAGG
GGTACAAGTTCTCCACTTACGCCACTTGGTGGATTCGTCAGGCCATCACCCGCGCCATCGCCGACCAGTCGCGCACCATC
CGCCTGCCGGTGCACCTCTACGAGACGATCTCTCGCATCAAGAAGACCACCAAGGTGCTCTCCCAGGAGTTCGGCCGCAA
GCCCACCGAAGAAGAGATCGCTGAATCGATGGAGATGACCATCGAGAAGCTGCGCTTCATCGCCAAGTCAGCTCAGTTGC
CGATTTCCCTGGAAACACCGATCGGTAAGGAAGAGGACTCACGTCTCGGCGACTTCATCGAAGCCGACATCGAGAATCCC
GACCAGGACGTGGCCAAGAACCTGCTGCGTGAGGACCTCGAAGGTGTGCTCGCCACCTTGAGCCCCCGTGAGCGTGATGT
GCTGCGTCTGCGTTATGGCCTCGACGATGGCCGCATGAAGACCCTCGAAGAGATCGGTCAGATCTTCGATGTGACCCGCG
AGCGCATCCGCCAGATCGAAGCCAAGGCCCTGCTCAAGTTGCGTCATCCCAACCGCAACGGCGTGCTCAAGGAGTACATC
AAATAA

Upstream 100 bases:

>100_bases
TACCCCAGACCACTGCTGTTGCGCAGGCGCGTTAGTCGCATGTGCTCGGCGTAATGAGACGCTTTCCCGTCTCTCTTAGC
CCTTCGTTCGGTTCGATCGC

Downstream 100 bases:

>100_bases
TCATTTACTCGATGTGCCAATCCTTCAGATCCTCTGCCTTGTAAAGGGTCTGAAGGTGTGACGATTCTGGCTCAAACGCT
GGAGATACGCCGGAGATACG

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MPVAAAAKSTTAVAEATEAKPVKAKAAPKAKAAPKAKAAPKAKAAPKAKAAPKAKAEPQAEVAVTTTVAKDGKAAKLPSI
KIGPKGVYTEDSIRVYLQEIGRIRLLRPDEEIELARKIADLLHLEELADQYEEDHGKYPDNREWAVLVDMPPIKFRRRLM
LGRRAKEKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTI
RLPVHLYETISRIKKTTKVLSQEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADIENP
DQDVAKNLLREDLEGVLATLSPRERDVLRLRYGLDDGRMKTLEEIGQIFDVTRERIRQIEAKALLKLRHPNRNGVLKEYI
K

Sequences:

>Translated_401_residues
MPVAAAAKSTTAVAEATEAKPVKAKAAPKAKAAPKAKAAPKAKAAPKAKAAPKAKAEPQAEVAVTTTVAKDGKAAKLPSI
KIGPKGVYTEDSIRVYLQEIGRIRLLRPDEEIELARKIADLLHLEELADQYEEDHGKYPDNREWAVLVDMPPIKFRRRLM
LGRRAKEKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTI
RLPVHLYETISRIKKTTKVLSQEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADIENP
DQDVAKNLLREDLEGVLATLSPRERDVLRLRYGLDDGRMKTLEEIGQIFDVTRERIRQIEAKALLKLRHPNRNGVLKEYI
K
>Mature_400_residues
PVAAAAKSTTAVAEATEAKPVKAKAAPKAKAAPKAKAAPKAKAAPKAKAAPKAKAEPQAEVAVTTTVAKDGKAAKLPSIK
IGPKGVYTEDSIRVYLQEIGRIRLLRPDEEIELARKIADLLHLEELADQYEEDHGKYPDNREWAVLVDMPPIKFRRRLML
GRRAKEKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIR
LPVHLYETISRIKKTTKVLSQEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADIENPD
QDVAKNLLREDLEGVLATLSPRERDVLRLRYGLDDGRMKTLEEIGQIFDVTRERIRQIEAKALLKLRHPNRNGVLKEYIK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=251, Percent_Identity=58.1673306772908, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1789098, Length=238, Percent_Identity=42.0168067226891, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=29.6296296296296, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=30.3921568627451, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 45176; Mature: 45045

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAAAAKSTTAVAEATEAKPVKAKAAPKAKAAPKAKAAPKAKAAPKAKAAPKAKAEPQA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EVAVTTTVAKDGKAAKLPSIKIGPKGVYTEDSIRVYLQEIGRIRLLRPDEEIELARKIAD
CEEEEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHH
LLHLEELADQYEEDHGKYPDNREWAVLVDMPPIKFRRRLMLGRRAKEKMVQSNLRLVVSI
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTI
HHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEE
RLPVHLYETISRIKKTTKVLSQEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETP
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
IGKEEDSRLGDFIEADIENPDQDVAKNLLREDLEGVLATLSPRERDVLRLRYGLDDGRMK
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
TLEEIGQIFDVTRERIRQIEAKALLKLRHPNRNGVLKEYIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
>Mature Secondary Structure 
PVAAAAKSTTAVAEATEAKPVKAKAAPKAKAAPKAKAAPKAKAAPKAKAAPKAKAEPQA
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EVAVTTTVAKDGKAAKLPSIKIGPKGVYTEDSIRVYLQEIGRIRLLRPDEEIELARKIAD
CEEEEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHH
LLHLEELADQYEEDHGKYPDNREWAVLVDMPPIKFRRRLMLGRRAKEKMVQSNLRLVVSI
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTI
HHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEE
RLPVHLYETISRIKKTTKVLSQEFGRKPTEEEIAESMEMTIEKLRFIAKSAQLPISLETP
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
IGKEEDSRLGDFIEADIENPDQDVAKNLLREDLEGVLATLSPRERDVLRLRYGLDDGRMK
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
TLEEIGQIFDVTRERIRQIEAKALLKLRHPNRNGVLKEYIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]