Click here to switch to the map view.

The map label for this gene is ycjG [C]

Identifier: 148241704

GI number: 148241704

Start: 539017

End: 540072

Strand: Direct

Name: ycjG [C]

Synonym: SynRCC307_0605

Alternate gene names: 148241704

Gene position: 539017-540072 (Clockwise)

Preceding gene: 148241697

Following gene: 148241705

Centisome position: 24.23

GC content: 66.19

Gene sequence:

>1056_bases
TTGGGTTGGCTTGCGATGCGCTGGTCACTGCGTCGTTTCACGCTGACCAAGCAGGTACCTCTCACCATCAGCCGCGGCAC
CACCGCTGCTGTGGAGCACCTGCTGTTGGAGCTTGAACACGGCGGCCTGATCGGCCGCGGCGAAACCGGTGGCTTTGACA
CCGGTCAGCGGGTTTTCAGCACCGCTCAGCTCGAGCAGGAGCTCACGGGCTGGTTACCGCAACTGGAGGCTGCTGGCTGG
CAGGAGCCGGCCCATTGGCCGGCATTGCTGGCGCCGCTGAGTGCGCCCGCACGCTGCGCGGTGGATTTGGCCTTGCATGA
TCTCTGGGGGCAGCTGTTGGCCCAGCCGCTGTGGCGGCTTTGGGGCCTGGACCTGCGCGGCTGCCAGCCCACCAGCGTCA
CCCTCGGGCTGGCGCCTGTGGAGAAGGTGCTGGAACGCTTGAGCCGTTGGCGTCAGCAGCTGCCGGCCAGCCGGGTGAAA
TTGAAGTTGGGCAGCCCCGATGGCCTCAGCCATGACCAGGCTCTGGTGGAGGCCCTGTTGCCAGTGCTGGGCGGGGCTGA
GTTGCAGGTGGATGCCAATGGCGGCTGGAGCCTGGAGGGCGCGCGCCAAATGCTGCCTTGGCTGGCGGAACGTGGGGTTG
TGCTGGTGGAGCAGCCGTTAGCGGCCAGCGATGGCGATGAGCAGGGGTTTGCGGCCCTGCAGGGGGCGGCTCCGATTCCC
TTGGTGGCCGATGAAAGCTGCTGGAACCTCGAGGATTTGTTGCGGCTGGCGCCCCACGTGCAGGGGGTCAACCTCAAGTT
GCTCAAAACCGGTGGTTTGAGCGAGGCGCTGTTGATGGCGCAGCTGGCCTCGCGGTTGGGCCTGAAGTTGATGCTGGGCT
GCTACTCCGATAGCGCCCTGCTCAATGGTGCTGCCGCCCAGCTGCTGCCCCTGGTGGAGTGGCCCGATCTCGATTCCCAT
CTCAACTTGGCTGATGACCCCTTCTCTGGCCCGCTGGCCCAGGGGGATCTGCTGCAGCCCAGTAGCGGGGCTGGTTTGGG
GGTGCGGCCATGTTGA

Upstream 100 bases:

>100_bases
CAGTAGGTCCCTCGAGAGGAGTCGGCCATGGGTGGATGAGGCTCAACCCAGTCTTGACCATGGCCGGTGAACTGACGTGG
CAGGGTGAGACACGACGGTC

Downstream 100 bases:

>100_bases
GCCCCGATGCACCGGTGCTGCTGCTGCAGCACGGCGGCCTCTTTGATCTGTCCGGCAAAACGGGCCTGGCGTTTTTGCGC
TACCGCCAGGGCCCTGTGGT

Product: L-alanine-DL-glutamate epimerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MGWLAMRWSLRRFTLTKQVPLTISRGTTAAVEHLLLELEHGGLIGRGETGGFDTGQRVFSTAQLEQELTGWLPQLEAAGW
QEPAHWPALLAPLSAPARCAVDLALHDLWGQLLAQPLWRLWGLDLRGCQPTSVTLGLAPVEKVLERLSRWRQQLPASRVK
LKLGSPDGLSHDQALVEALLPVLGGAELQVDANGGWSLEGARQMLPWLAERGVVLVEQPLAASDGDEQGFAALQGAAPIP
LVADESCWNLEDLLRLAPHVQGVNLKLLKTGGLSEALLMAQLASRLGLKLMLGCYSDSALLNGAAAQLLPLVEWPDLDSH
LNLADDPFSGPLAQGDLLQPSSGAGLGVRPC

Sequences:

>Translated_351_residues
MGWLAMRWSLRRFTLTKQVPLTISRGTTAAVEHLLLELEHGGLIGRGETGGFDTGQRVFSTAQLEQELTGWLPQLEAAGW
QEPAHWPALLAPLSAPARCAVDLALHDLWGQLLAQPLWRLWGLDLRGCQPTSVTLGLAPVEKVLERLSRWRQQLPASRVK
LKLGSPDGLSHDQALVEALLPVLGGAELQVDANGGWSLEGARQMLPWLAERGVVLVEQPLAASDGDEQGFAALQGAAPIP
LVADESCWNLEDLLRLAPHVQGVNLKLLKTGGLSEALLMAQLASRLGLKLMLGCYSDSALLNGAAAQLLPLVEWPDLDSH
LNLADDPFSGPLAQGDLLQPSSGAGLGVRPC
>Mature_350_residues
GWLAMRWSLRRFTLTKQVPLTISRGTTAAVEHLLLELEHGGLIGRGETGGFDTGQRVFSTAQLEQELTGWLPQLEAAGWQ
EPAHWPALLAPLSAPARCAVDLALHDLWGQLLAQPLWRLWGLDLRGCQPTSVTLGLAPVEKVLERLSRWRQQLPASRVKL
KLGSPDGLSHDQALVEALLPVLGGAELQVDANGGWSLEGARQMLPWLAERGVVLVEQPLAASDGDEQGFAALQGAAPIPL
VADESCWNLEDLLRLAPHVQGVNLKLLKTGGLSEALLMAQLASRLGLKLMLGCYSDSALLNGAAAQLLPLVEWPDLDSHL
NLADDPFSGPLAQGDLLQPSSGAGLGVRPC

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of a broad range of other

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=304, Percent_Identity=34.2105263157895, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 37620; Mature: 37489

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWLAMRWSLRRFTLTKQVPLTISRGTTAAVEHLLLELEHGGLIGRGETGGFDTGQRVFS
CCCEEHHHHHHHEEEECCCCEEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHH
TAQLEQELTGWLPQLEAAGWQEPAHWPALLAPLSAPARCAVDLALHDLWGQLLAQPLWRL
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WGLDLRGCQPTSVTLGLAPVEKVLERLSRWRQQLPASRVKLKLGSPDGLSHDQALVEALL
HCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
PVLGGAELQVDANGGWSLEGARQMLPWLAERGVVLVEQPLAASDGDEQGFAALQGAAPIP
HHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHCCCCCCC
LVADESCWNLEDLLRLAPHVQGVNLKLLKTGGLSEALLMAQLASRLGLKLMLGCYSDSAL
EECCCCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCHHH
LNGAAAQLLPLVEWPDLDSHLNLADDPFSGPLAQGDLLQPSSGAGLGVRPC
HCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GWLAMRWSLRRFTLTKQVPLTISRGTTAAVEHLLLELEHGGLIGRGETGGFDTGQRVFS
CCEEHHHHHHHEEEECCCCEEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHH
TAQLEQELTGWLPQLEAAGWQEPAHWPALLAPLSAPARCAVDLALHDLWGQLLAQPLWRL
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WGLDLRGCQPTSVTLGLAPVEKVLERLSRWRQQLPASRVKLKLGSPDGLSHDQALVEALL
HCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
PVLGGAELQVDANGGWSLEGARQMLPWLAERGVVLVEQPLAASDGDEQGFAALQGAAPIP
HHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHCCCCCCC
LVADESCWNLEDLLRLAPHVQGVNLKLLKTGGLSEALLMAQLASRLGLKLMLGCYSDSAL
EECCCCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCHHH
LNGAAAQLLPLVEWPDLDSHLNLADDPFSGPLAQGDLLQPSSGAGLGVRPC
HCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]