Click here to switch to the map view.

The map label for this gene is walK [H]

Identifier: 148241664

GI number: 148241664

Start: 499851

End: 500942

Strand: Direct

Name: walK [H]

Synonym: SynRCC307_0565

Alternate gene names: 148241664

Gene position: 499851-500942 (Clockwise)

Preceding gene: 148241663

Following gene: 148241666

Centisome position: 22.47

GC content: 59.52

Gene sequence:

>1092_bases
ATGAGTGAGGAGGCCACGCTGGCTCAGTTGCGCCAGTCGCTTGGCCTGCTGCAGGTGGCGTTTGATGCGGCAGCAGAGGC
GATGGTGATTGTTGAGCCTGGCGGGGAGGTGCGTTGGGGGAATCAAGCCGCCGCCGATCTTTGGTCCAATGGCTTGGCGA
TCCTGTTGGTGGGCCAGCGGTTGGAGCAGTTGCTTCAACCCATCTGCAGCAGCAGCGGTGTGCCGTTAGCTCTTGAGGCT
GCCGAGCATCCGCTGCAACTCCTCAAGCGTGGGGATGGCTCAGGTGTTTTTGGAATCGCTGATCATTTGCAGCAGTTGGA
GTGGCGGCGGATTCAGCAGCCTGGCGATGGCTACCTGTTGCTGTTGGCGCGTGATTTGGCTCCGCAAGAGCAGGCGTTAC
AGCAACAGCGCCAATTTGTGAACCAGTTGGCCCATGAGCTCAACACGCCATTGGCGATCGTCAACGGCAGCCTGCGGCAG
CTGGGCAAGAAGCTGCTGAATTTCGGTGATGGATCACGCGAGCGCTTGCGCCAGGCGCAGCAGGAAACCAGCCGGCTGGT
GCGATTGCTGCGCCATCTTTTGGTGCTGACGGATCTGGAAACAGGCCGCCGCCAGCTACAGCTCCAGCCTTTGGCCCTCA
AGCCTTGGCTGCAGGAGTGGATGGCCCAGCAAGTTCTGCCTGCTTCTGCTGAATTGAATCTTGTGGCTGATGTTGGCGAG
AGCGCTGTTATTGCCGCTGATGCCGAGGCATTGGCCGAGGTGTTGGAGCAATTGCTCGATAACAGCCTGCGTTACAGCAA
AGGGCCTGCTCAGATCGAGCTGCAACTGCTGGAGCTGGATGGAGCGAGCGGTTTGCGCTGGCAAGACCAAGGCCTCGGCA
TCACAGCTGAACCGCGCAGCCAGGTGTTTGAGCGCTTTGTTCGATTGGAGGAACATCGCGATTTCAACCAGGCCGACGGA
GCTGGTCTGGGACTGGCTTTGTGTGAGGCCTTGATGCAAGCCATGGGAGGCAGCACTGGACTAGAGCCAATCACCAACGA
ATCGACGGGCACCGCTTTCCTATTGAGCTGGCCTCAGGCTGGAGGCAAGTAG

Upstream 100 bases:

>100_bases
AAAGCGAGCACTACATCGTTGCTGAGAAATATCCCGATGGCAGTTGGAGCTTTGCCGCCACATTGCGGCCATTGGAAGGT
GAGCACGCTTCAGATCAGGA

Downstream 100 bases:

>100_bases
CGGTTCAAAACGGCTTCAACGTTGTCGAATAGCGCTGCTTTGAGCATCCATCCGTTAGCCCCAGCTGCTTTGGCTTGCTC
CTGTAGCTCAGCGTCGTTCA

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSEEATLAQLRQSLGLLQVAFDAAAEAMVIVEPGGEVRWGNQAAADLWSNGLAILLVGQRLEQLLQPICSSSGVPLALEA
AEHPLQLLKRGDGSGVFGIADHLQQLEWRRIQQPGDGYLLLLARDLAPQEQALQQQRQFVNQLAHELNTPLAIVNGSLRQ
LGKKLLNFGDGSRERLRQAQQETSRLVRLLRHLLVLTDLETGRRQLQLQPLALKPWLQEWMAQQVLPASAELNLVADVGE
SAVIAADAEALAEVLEQLLDNSLRYSKGPAQIELQLLELDGASGLRWQDQGLGITAEPRSQVFERFVRLEEHRDFNQADG
AGLGLALCEALMQAMGGSTGLEPITNESTGTAFLLSWPQAGGK

Sequences:

>Translated_363_residues
MSEEATLAQLRQSLGLLQVAFDAAAEAMVIVEPGGEVRWGNQAAADLWSNGLAILLVGQRLEQLLQPICSSSGVPLALEA
AEHPLQLLKRGDGSGVFGIADHLQQLEWRRIQQPGDGYLLLLARDLAPQEQALQQQRQFVNQLAHELNTPLAIVNGSLRQ
LGKKLLNFGDGSRERLRQAQQETSRLVRLLRHLLVLTDLETGRRQLQLQPLALKPWLQEWMAQQVLPASAELNLVADVGE
SAVIAADAEALAEVLEQLLDNSLRYSKGPAQIELQLLELDGASGLRWQDQGLGITAEPRSQVFERFVRLEEHRDFNQADG
AGLGLALCEALMQAMGGSTGLEPITNESTGTAFLLSWPQAGGK
>Mature_362_residues
SEEATLAQLRQSLGLLQVAFDAAAEAMVIVEPGGEVRWGNQAAADLWSNGLAILLVGQRLEQLLQPICSSSGVPLALEAA
EHPLQLLKRGDGSGVFGIADHLQQLEWRRIQQPGDGYLLLLARDLAPQEQALQQQRQFVNQLAHELNTPLAIVNGSLRQL
GKKLLNFGDGSRERLRQAQQETSRLVRLLRHLLVLTDLETGRRQLQLQPLALKPWLQEWMAQQVLPASAELNLVADVGES
AVIAADAEALAEVLEQLLDNSLRYSKGPAQIELQLLELDGASGLRWQDQGLGITAEPRSQVFERFVRLEEHRDFNQADGA
GLGLALCEALMQAMGGSTGLEPITNESTGTAFLLSWPQAGGK

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: COG5002

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=228, Percent_Identity=31.140350877193, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788393, Length=209, Percent_Identity=29.6650717703349, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=26.3829787234043, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786600, Length=358, Percent_Identity=23.7430167597765, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790346, Length=218, Percent_Identity=28.8990825688073, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789149, Length=291, Percent_Identity=24.7422680412371, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786912, Length=307, Percent_Identity=25.0814332247557, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39607; Mature: 39476

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEATLAQLRQSLGLLQVAFDAAAEAMVIVEPGGEVRWGNQAAADLWSNGLAILLVGQR
CCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEECCCHHHHHHHCCCCEEEEHHHH
LEQLLQPICSSSGVPLALEAAEHPLQLLKRGDGSGVFGIADHLQQLEWRRIQQPGDGYLL
HHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEE
LLARDLAPQEQALQQQRQFVNQLAHELNTPLAIVNGSLRQLGKKLLNFGDGSRERLRQAQ
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHH
QETSRLVRLLRHLLVLTDLETGRRQLQLQPLALKPWLQEWMAQQVLPASAELNLVADVGE
HHHHHHHHHHHHHHHHHCCHHCHHHEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEECCC
SAVIAADAEALAEVLEQLLDNSLRYSKGPAQIELQLLELDGASGLRWQDQGLGITAEPRS
CEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHH
QVFERFVRLEEHRDFNQADGAGLGLALCEALMQAMGGSTGLEPITNESTGTAFLLSWPQA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
GGK
CCC
>Mature Secondary Structure 
SEEATLAQLRQSLGLLQVAFDAAAEAMVIVEPGGEVRWGNQAAADLWSNGLAILLVGQR
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEECCCHHHHHHHCCCCEEEEHHHH
LEQLLQPICSSSGVPLALEAAEHPLQLLKRGDGSGVFGIADHLQQLEWRRIQQPGDGYLL
HHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEE
LLARDLAPQEQALQQQRQFVNQLAHELNTPLAIVNGSLRQLGKKLLNFGDGSRERLRQAQ
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHH
QETSRLVRLLRHLLVLTDLETGRRQLQLQPLALKPWLQEWMAQQVLPASAELNLVADVGE
HHHHHHHHHHHHHHHHHCCHHCHHHEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEECCC
SAVIAADAEALAEVLEQLLDNSLRYSKGPAQIELQLLELDGASGLRWQDQGLGITAEPRS
CEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHH
QVFERFVRLEEHRDFNQADGAGLGLALCEALMQAMGGSTGLEPITNESTGTAFLLSWPQA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
GGK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA