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The map label for this gene is ascG [H]

Identifier: 147675752

GI number: 147675752

Start: 1221820

End: 1222854

Strand: Reverse

Name: ascG [H]

Synonym: VC0395_A1164

Alternate gene names: 147675752

Gene position: 1222854-1221820 (Counterclockwise)

Preceding gene: 147673845

Following gene: 147675253

Centisome position: 40.44

GC content: 48.5

Gene sequence:

>1035_bases
TTGCGGACTCAGTTTGAAGGATTGTTGATGGCAACCATAAACGATGTGTGTAAATTGGCGGGTGTCTCCAAAGCCACGGT
ATCACGAGTGCTGAATGAGACAGGCCAAGTCAAAGCACAAACGCGTGAAGCGGTACTTGCCGCCATGCAGCAGCTCGGTT
ATCAGCCCAACAGTTTGGCGCAGGCGCTCGCTACCAATACCACAAACTCAATTGGCTTGGTGCTGCCGCATTTTGAGAGC
AGCTATTTTGGTTCGATTTTGTTTGAAGCCGAACAAGGCGCGCAGAAAGCTGGGAAAAAACTGCTGGTTATGAACAGCAA
AAACAGTGAGCAAGGCGAAAAAGAAGCGGTCGCCACATTAGCGGCGCAGCGTTGTGATGCGATCTTGCTGTACAGTCGTC
ATTTGAATGAGGTGCAATTACTTGAATTACAACAGCAACATCCATCGCCCTTAGTGATCCTCAATCGCCGTTTACATCAC
CCGCAATTGCACAGCTTTGGGCTCGATCAAACGCAAATTGCCCAGTTAGCGATGCAGCATTTGTTGAATTTAGGGCATCG
TCAAATTGCCTGCATTACATCGCCGTTGGTGAGCGAGACGGGAAAAATTCGTTATCAGGTCTATCAACAAGCGTTACATG
AGCAAGGGATAGAGTTGAGCTCGTCCTTGGTCATTGAAGGGGATAACACGCTGCTTGGTGGTTATCAGGCAATGCAACAA
CTGCTGCAACAAGGCATTTCCATGACGGCTGTTTTTGCCTGTAACGATGATATGGCGCTCGGTGCAATGCGTGCCATGCA
TGAACATGGCATTCATGTACCCAAACAAGTTTCCATAATCGGGATTGATAATGAACCCGCGGCGGCTTTTGCTATCCCGA
GTTTATCGAGCGTGAGTCTACCGATCGGCGAGTTAACTCAGCAAGCAATCAGTTTGGCTGTCGAGATTGCTAATAAAAAA
CCACAAGATGCACAGCACAGGCTCTATCAAGGGAGTTTGATCGCCCGTGAATCCACCATAGCGCTCAAACTATGA

Upstream 100 bases:

>100_bases
GCGCTAAGAAAAAGAGCTTCTACTGGTATCAACAAGTGATCGCCAGTAACGGTGAAAAGCTTTAAATCGTGTCGGCGGCC
TTTGGGGCGCCGCTTAATTT

Downstream 100 bases:

>100_bases
GTAAAACAGCAACGTGTCAGTAAAATTGCGGGGACAGAGCGCGATTTTACGCTTCATCTTGGCTATCCTGCTCCCCGATA
TAGATAAGTGAAGAGGCGAC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRTQFEGLLMATINDVCKLAGVSKATVSRVLNETGQVKAQTREAVLAAMQQLGYQPNSLAQALATNTTNSIGLVLPHFES
SYFGSILFEAEQGAQKAGKKLLVMNSKNSEQGEKEAVATLAAQRCDAILLYSRHLNEVQLLELQQQHPSPLVILNRRLHH
PQLHSFGLDQTQIAQLAMQHLLNLGHRQIACITSPLVSETGKIRYQVYQQALHEQGIELSSSLVIEGDNTLLGGYQAMQQ
LLQQGISMTAVFACNDDMALGAMRAMHEHGIHVPKQVSIIGIDNEPAAAFAIPSLSSVSLPIGELTQQAISLAVEIANKK
PQDAQHRLYQGSLIARESTIALKL

Sequences:

>Translated_344_residues
MRTQFEGLLMATINDVCKLAGVSKATVSRVLNETGQVKAQTREAVLAAMQQLGYQPNSLAQALATNTTNSIGLVLPHFES
SYFGSILFEAEQGAQKAGKKLLVMNSKNSEQGEKEAVATLAAQRCDAILLYSRHLNEVQLLELQQQHPSPLVILNRRLHH
PQLHSFGLDQTQIAQLAMQHLLNLGHRQIACITSPLVSETGKIRYQVYQQALHEQGIELSSSLVIEGDNTLLGGYQAMQQ
LLQQGISMTAVFACNDDMALGAMRAMHEHGIHVPKQVSIIGIDNEPAAAFAIPSLSSVSLPIGELTQQAISLAVEIANKK
PQDAQHRLYQGSLIARESTIALKL
>Mature_344_residues
MRTQFEGLLMATINDVCKLAGVSKATVSRVLNETGQVKAQTREAVLAAMQQLGYQPNSLAQALATNTTNSIGLVLPHFES
SYFGSILFEAEQGAQKAGKKLLVMNSKNSEQGEKEAVATLAAQRCDAILLYSRHLNEVQLLELQQQHPSPLVILNRRLHH
PQLHSFGLDQTQIAQLAMQHLLNLGHRQIACITSPLVSETGKIRYQVYQQALHEQGIELSSSLVIEGDNTLLGGYQAMQQ
LLQQGISMTAVFACNDDMALGAMRAMHEHGIHVPKQVSIIGIDNEPAAAFAIPSLSSVSLPIGELTQQAISLAVEIANKK
PQDAQHRLYQGSLIARESTIALKL

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=38.2089552238806, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=37.4622356495468, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1789202, Length=329, Percent_Identity=37.3860182370821, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=34.1389728096677, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=30.7228915662651, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=32.2289156626506, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786540, Length=332, Percent_Identity=30.7228915662651, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=27.2151898734177, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994940, Length=290, Percent_Identity=26.8965517241379, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790715, Length=326, Percent_Identity=25.1533742331288, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787906, Length=325, Percent_Identity=25.5384615384615, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789456, Length=332, Percent_Identity=24.3975903614458, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786268, Length=169, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790689, Length=300, Percent_Identity=26.3333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790192, Length=264, Percent_Identity=25.7575757575758, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37437; Mature: 37437

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTQFEGLLMATINDVCKLAGVSKATVSRVLNETGQVKAQTREAVLAAMQQLGYQPNSLA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHH
QALATNTTNSIGLVLPHFESSYFGSILFEAEQGAQKAGKKLLVMNSKNSEQGEKEAVATL
HHHHHCCCCCCEEEECCCCCCHHHHEEEEHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
AAQRCDAILLYSRHLNEVQLLELQQQHPSPLVILNRRLHHPQLHSFGLDQTQIAQLAMQH
HHHHCCEEEEEHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHCCCCHHHHHHHHHHH
LLNLGHRQIACITSPLVSETGKIRYQVYQQALHEQGIELSSSLVIEGDNTLLGGYQAMQQ
HHHCCCCEEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECHHHHHHH
LLQQGISMTAVFACNDDMALGAMRAMHEHGIHVPKQVSIIGIDNEPAAAFAIPSLSSVSL
HHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCCCCCCC
PIGELTQQAISLAVEIANKKPQDAQHRLYQGSLIARESTIALKL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCEEEEEC
>Mature Secondary Structure
MRTQFEGLLMATINDVCKLAGVSKATVSRVLNETGQVKAQTREAVLAAMQQLGYQPNSLA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHH
QALATNTTNSIGLVLPHFESSYFGSILFEAEQGAQKAGKKLLVMNSKNSEQGEKEAVATL
HHHHHCCCCCCEEEECCCCCCHHHHEEEEHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
AAQRCDAILLYSRHLNEVQLLELQQQHPSPLVILNRRLHHPQLHSFGLDQTQIAQLAMQH
HHHHCCEEEEEHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHCCCCHHHHHHHHHHH
LLNLGHRQIACITSPLVSETGKIRYQVYQQALHEQGIELSSSLVIEGDNTLLGGYQAMQQ
HHHCCCCEEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECHHHHHHH
LLQQGISMTAVFACNDDMALGAMRAMHEHGIHVPKQVSIIGIDNEPAAAFAIPSLSSVSL
HHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCCCCCCC
PIGELTQQAISLAVEIANKKPQDAQHRLYQGSLIARESTIALKL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]