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The map label for this gene is pdxJ [H]

Identifier: 147675577

GI number: 147675577

Start: 2194188

End: 2194919

Strand: Reverse

Name: pdxJ [H]

Synonym: VC0395_A2036

Alternate gene names: 147675577

Gene position: 2194919-2194188 (Counterclockwise)

Preceding gene: 147673381

Following gene: 147675265

Centisome position: 72.58

GC content: 51.64

Gene sequence:

>732_bases
ATGAGCTCAATTTATCTTGGTGTGAATATCGATCACGTGGCGACTTTGCGTAACGCACGCGGCACACAATATCCCGATCC
AGTGCATGCAGCGGAAATTGCTGAACGTGCGGGCGCGGATGGCATCACCATCCATCTGCGTGAAGATCGTCGTCACATCA
CAGATCGCGATGTCCGTATTTTGCGTGAAACGCTGCAAACGCGCATGAACCTTGAAATGGCGGTGACGGATGAGATGGTC
GAGATTGCGCTGCAAACTCAGCCAGAATACGTCTGCTTGGTGCCGGAAAAACGTGAAGAGTTGACCACTGAAGGCGGTCT
TGATGTGCTAGGTCAGTTGGAGCGTGTCAAAGCGGCAACGGAAAAACTCACTGCGGCTGGCATTAAAGTTTCTCTGTTTA
TCGATGCCGATCGTGAGCAAATTGATGCGGCCAAAGCTTGTGGCGCACCTTTTATTGAGCTGCATACCGGGCACTATTCC
GATGCGAAAAGCGATGTCGATCAGCAAAATGAGCTGAAGAAAATTGCAGCCGCAGCGGCTTACGCACACGATTTAGGCAT
TACGGTGAATGCGGGGCATGGCTTGACCTACCACAATGTCGCGGCGATTGCGGCCATTCCTGAAATTTACGAGCTGAACA
TCGGCCATGCGATCATTGGTCGCGCGGTATTTGATGGTTTAGCGAAAGCCGTCTCCGATATGAAAGCCATCATGGTTGCT
GCGCGTCGATAA

Upstream 100 bases:

>100_bases
TTACACGTTTAGCCTTAAAGCCGTATCTTGGCGGCAAACCTTTAAAGAGTCGTGAGTTGTTTCGTCAAACAACTCTACCT
AGAGCACGGAGTACAGAAGA

Downstream 100 bases:

>100_bases
CGTATGATCGTTGGACTCGGAACGGATATCGCTGAAATCGAACGGGTTGAGAAAGCGTTGGCGCGCTCAGGAGAAAACTT
TGCGCGCCGCATTCTTACCG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSSIYLGVNIDHVATLRNARGTQYPDPVHAAEIAERAGADGITIHLREDRRHITDRDVRILRETLQTRMNLEMAVTDEMV
EIALQTQPEYVCLVPEKREELTTEGGLDVLGQLERVKAATEKLTAAGIKVSLFIDADREQIDAAKACGAPFIELHTGHYS
DAKSDVDQQNELKKIAAAAAYAHDLGITVNAGHGLTYHNVAAIAAIPEIYELNIGHAIIGRAVFDGLAKAVSDMKAIMVA
ARR

Sequences:

>Translated_243_residues
MSSIYLGVNIDHVATLRNARGTQYPDPVHAAEIAERAGADGITIHLREDRRHITDRDVRILRETLQTRMNLEMAVTDEMV
EIALQTQPEYVCLVPEKREELTTEGGLDVLGQLERVKAATEKLTAAGIKVSLFIDADREQIDAAKACGAPFIELHTGHYS
DAKSDVDQQNELKKIAAAAAYAHDLGITVNAGHGLTYHNVAAIAAIPEIYELNIGHAIIGRAVFDGLAKAVSDMKAIMVA
ARR
>Mature_242_residues
SSIYLGVNIDHVATLRNARGTQYPDPVHAAEIAERAGADGITIHLREDRRHITDRDVRILRETLQTRMNLEMAVTDEMVE
IALQTQPEYVCLVPEKREELTTEGGLDVLGQLERVKAATEKLTAAGIKVSLFIDADREQIDAAKACGAPFIELHTGHYSD
AKSDVDQQNELKKIAAAAAYAHDLGITVNAGHGLTYHNVAAIAAIPEIYELNIGHAIIGRAVFDGLAKAVSDMKAIMVAA
RR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=242, Percent_Identity=70.6611570247934, Blast_Score=355, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 26470; Mature: 26339

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIYLGVNIDHVATLRNARGTQYPDPVHAAEIAERAGADGITIHLREDRRHITDRDVRI
CCEEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
LRETLQTRMNLEMAVTDEMVEIALQTQPEYVCLVPEKREELTTEGGLDVLGQLERVKAAT
HHHHHHHHHCCCEEEHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCHHHHHHHHHHHHHH
EKLTAAGIKVSLFIDADREQIDAAKACGAPFIELHTGHYSDAKSDVDQQNELKKIAAAAA
HHHHHCCEEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCHHCCHHHHHHHHHHHHHH
YAHDLGITVNAGHGLTYHNVAAIAAIPEIYELNIGHAIIGRAVFDGLAKAVSDMKAIMVA
HHHHCCEEEECCCCCCHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARR
CCC
>Mature Secondary Structure 
SSIYLGVNIDHVATLRNARGTQYPDPVHAAEIAERAGADGITIHLREDRRHITDRDVRI
CEEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
LRETLQTRMNLEMAVTDEMVEIALQTQPEYVCLVPEKREELTTEGGLDVLGQLERVKAAT
HHHHHHHHHCCCEEEHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCHHHHHHHHHHHHHH
EKLTAAGIKVSLFIDADREQIDAAKACGAPFIELHTGHYSDAKSDVDQQNELKKIAAAAA
HHHHHCCEEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCHHCCHHHHHHHHHHHHHH
YAHDLGITVNAGHGLTYHNVAAIAAIPEIYELNIGHAIIGRAVFDGLAKAVSDMKAIMVA
HHHHCCEEEECCCCCCHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]