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The map label for this gene is tnpA [H]

Identifier: 147675515

GI number: 147675515

Start: 426657

End: 427094

Strand: Direct

Name: tnpA [H]

Synonym: VC0395_A0396

Alternate gene names: 147675515

Gene position: 426657-427094 (Clockwise)

Preceding gene: 147673351

Following gene: 147673049

Centisome position: 14.11

GC content: 38.81

Gene sequence:

>438_bases
ATGGGCGACTACAGAAGTTCATCACACGTCTATTGGCGTTGCAAATATCATATCGTTTGGACACCAAAATTTCGTTTTAA
GATCTTAAAAGGTAATGTAGCCAAAGAGCTAAATCGTTCGATCTACATTCTTTGTAATATGAAAGATTGTGAAGTTTTGG
AACTCAATGTTCAGCCAGATCATGTCCACTTAGTTGCGATAATTCCGCCCAAAGTATCGATTTCGACGTTGATGGGAGTT
TTAAAGGGTAGGAGTGCAATTAGGCTATTCAACAAGTTTCCACATATCAGGAAAAAGTTATGGGGAAATCATTTTTGGGC
GCGAGGCTATTTTGTGGATACGGTAGGTGTAAATGAAGAAATCATTAGACGGTATGTACGGCATCAAGACAAAAAAGAGC
TTGAGCAAGAGCAGCAGTTAGAGTTATTGAGAGACTAA

Upstream 100 bases:

>100_bases
CTTTTAGCGGTGGTGATTGTCCCTAGGGGCTTTTGCCCGAAAATGCGCCCATGTTAGAAGACAAACTCTTATTCACCATA
AGTAAGAGGATTCAAATAAC

Downstream 100 bases:

>100_bases
CAGCGCCGTGGCCCCCTTTTAGGGGGCTTATATTAAAACCGCCTTCTAAGAAGGCGGATTTTTATTTTTTCAGCCCCGAT
TTGAAAGGGCGCTTTGCGAT

Product: IS1004 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MGDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPDHVHLVAIIPPKVSISTLMGV
LKGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEEIIRRYVRHQDKKELEQEQQLELLRD

Sequences:

>Translated_145_residues
MGDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPDHVHLVAIIPPKVSISTLMGV
LKGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEEIIRRYVRHQDKKELEQEQQLELLRD
>Mature_144_residues
GDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPDHVHLVAIIPPKVSISTLMGVL
KGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEEIIRRYVRHQDKKELEQEQQLELLRD

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: COG1943

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 17 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002686 [H]

Pfam domain/function: PF01797 Transposase_17 [H]

EC number: NA

Molecular weight: Translated: 17321; Mature: 17189

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPD
CCCCCCCCEEEEEEEEEEEECCCEEEEEECCCHHHHCCCEEEEEEECCCCEEEEEECCCC
HVHLVAIIPPKVSISTLMGVLKGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEE
EEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHCCCCEEECCEEEEECCCCHH
IIRRYVRHQDKKELEQEQQLELLRD
HHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPD
CCCCCCCEEEEEEEEEEEECCCEEEEEECCCHHHHCCCEEEEEEECCCCEEEEEECCCC
HVHLVAIIPPKVSISTLMGVLKGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEE
EEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHCCCCEEECCEEEEECCCCHH
IIRRYVRHQDKKELEQEQQLELLRD
HHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]