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The map label for this gene is epsK

Identifier: 147675385

GI number: 147675385

Start: 2453220

End: 2454230

Strand: Reverse

Name: epsK

Synonym: VC0395_A2299

Alternate gene names: 147675385

Gene position: 2454230-2453220 (Counterclockwise)

Preceding gene: 147674823

Following gene: 147673681

Centisome position: 81.16

GC content: 52.32

Gene sequence:

>1011_bases
ATGCGGGCTAAACAGCGCGGCGTGGCGTTAATTGTGATTCTGCTCCTGCTGGCGGTGATGGTCTCGATTGCTGCGACCAT
GGCCGAACGTCTGTTTAGTCAGTTTCAGCGTGCCACGCATCAGCTCAACTATCAGCAGGCTTATTGGTACAGTCTTGGCG
TCGAAGCGCTAGCGAAAAAAGGCATTGAGCAGAGCTACCAAGACAGTGAAACCATCAATTTGAGTCAGCCTTGGGCTTTG
AAAGAGCAAACTTATCCGCTCGATTACGGACAGGTGCGCGGCAAAATCCGCGATATGCAAGCTTGCTTTAATCTCAATGC
ACTCGCGGGGGTAAAGCTCACGCCAGACAGCGTGAAAAAACCGTATTTGCTGACGGTGCTGCAAGCGCTGCTTGAAGGGC
TGGAAGTGGAGAGTTATCAAGCGGAAGTGATTGCCGATTCGACGTTAGAGTTTATTGATAAAGATGACTCTGTACGCACC
GCTTATGGGGTGGAAGACAGTTACTATGAATCGATGATCCCGGCCTATATGGCGGCGGATACTTGGTTGGCCGATGCGAG
CGAGTGGCGTGCGGTACAGCAAGTGGGGGGAGAAACGATGAATAAAGCCTTACCTTATGTGTGTGCGTTGCCAACCGATC
AATGGCGCTTGAATGTGAACACGTTACCCGCTGAGCAAGCGGCACTGCTGGCGGCCATGTTTAGCCCAACATTGAGTCCG
GAAAGCGCGAAAACCTTGCTCGAAGGGCGGCCTTTCGATGGTTGGGCGAGTGTGGATGATTTTCTTGCCCAATCGGCACT
CACCGGAGTGGATAACGCGGTGCGTGAGGAAGCGAAGAAATACCTCAGTGTAGATAGCCATTATTTTGAATTAGATGCAC
AGGTGCTGGTCGATACGTCTCGCGTGCGGATCCGCAGCCTGTTTTACAGTAACGATAAGAAAACTGCGACGGTGATACGC
CGCCGCTTTGGAGGGATCAGTGAGCGAGTTTCTGACCGTTCGACTGAGTAG

Upstream 100 bases:

>100_bases
GCCGTGAAAGTGACCTTGCGCCTAAAAGATTACGGTGAGATTGAGCGGATATACCTTACAGGGGGCGGTTCACTCAATAT
GACGCAAGAGAGTGTTGAAA

Downstream 100 bases:

>100_bases
TCAAAAAGAGGCCGATATCCCTTGGCTGGTTTGGTCTGCCGAGCAGCAAGAAGTGATTGCCAGCGGCCAAGTGGCTGGTT
GGGAAGCCTTGCATGAAATT

Product: general secretion pathway protein K

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MRAKQRGVALIVILLLLAVMVSIAATMAERLFSQFQRATHQLNYQQAYWYSLGVEALAKKGIEQSYQDSETINLSQPWAL
KEQTYPLDYGQVRGKIRDMQACFNLNALAGVKLTPDSVKKPYLLTVLQALLEGLEVESYQAEVIADSTLEFIDKDDSVRT
AYGVEDSYYESMIPAYMAADTWLADASEWRAVQQVGGETMNKALPYVCALPTDQWRLNVNTLPAEQAALLAAMFSPTLSP
ESAKTLLEGRPFDGWASVDDFLAQSALTGVDNAVREEAKKYLSVDSHYFELDAQVLVDTSRVRIRSLFYSNDKKTATVIR
RRFGGISERVSDRSTE

Sequences:

>Translated_336_residues
MRAKQRGVALIVILLLLAVMVSIAATMAERLFSQFQRATHQLNYQQAYWYSLGVEALAKKGIEQSYQDSETINLSQPWAL
KEQTYPLDYGQVRGKIRDMQACFNLNALAGVKLTPDSVKKPYLLTVLQALLEGLEVESYQAEVIADSTLEFIDKDDSVRT
AYGVEDSYYESMIPAYMAADTWLADASEWRAVQQVGGETMNKALPYVCALPTDQWRLNVNTLPAEQAALLAAMFSPTLSP
ESAKTLLEGRPFDGWASVDDFLAQSALTGVDNAVREEAKKYLSVDSHYFELDAQVLVDTSRVRIRSLFYSNDKKTATVIR
RRFGGISERVSDRSTE
>Mature_336_residues
MRAKQRGVALIVILLLLAVMVSIAATMAERLFSQFQRATHQLNYQQAYWYSLGVEALAKKGIEQSYQDSETINLSQPWAL
KEQTYPLDYGQVRGKIRDMQACFNLNALAGVKLTPDSVKKPYLLTVLQALLEGLEVESYQAEVIADSTLEFIDKDDSVRT
AYGVEDSYYESMIPAYMAADTWLADASEWRAVQQVGGETMNKALPYVCALPTDQWRLNVNTLPAEQAALLAAMFSPTLSP
ESAKTLLEGRPFDGWASVDDFLAQSALTGVDNAVREEAKKYLSVDSHYFELDAQVLVDTSRVRIRSLFYSNDKKTATVIR
RRFGGISERVSDRSTE

Specific function: Required for secretion of cholera toxin through the outer membrane

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family

Homologues:

Organism=Escherichia coli, GI1789729, Length=327, Percent_Identity=34.5565749235474, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSPK_VIBCH (P45781)

Other databases:

- EMBL:   L33796
- EMBL:   AE003852
- PIR:   H82040
- RefSeq:   NP_232353.1
- ProteinModelPortal:   P45781
- SMR:   P45781
- GeneID:   2614889
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2726
- TIGR:   VC_2726
- HOGENOM:   HBG638472
- OMA:   DKQACFN
- ProtClustDB:   CLSK874897
- GO:   GO:0006810
- InterPro:   IPR005628

Pfam domain/function: PF03934 GspK

EC number: NA

Molecular weight: Translated: 37602; Mature: 37602

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b530e2c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAKQRGVALIVILLLLAVMVSIAATMAERLFSQFQRATHQLNYQQAYWYSLGVEALAKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GIEQSYQDSETINLSQPWALKEQTYPLDYGQVRGKIRDMQACFNLNALAGVKLTPDSVKK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCEECHHHHCC
PYLLTVLQALLEGLEVESYQAEVIADSTLEFIDKDDSVRTAYGVEDSYYESMIPAYMAAD
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHH
TWLADASEWRAVQQVGGETMNKALPYVCALPTDQWRLNVNTLPAEQAALLAAMFSPTLSP
HHHHCHHHHHHHHHHCHHHHHHHCCEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
ESAKTLLEGRPFDGWASVDDFLAQSALTGVDNAVREEAKKYLSVDSHYFELDAQVLVDTS
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECHHHEECHH
RVRIRSLFYSNDKKTATVIRRRFGGISERVSDRSTE
HHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCH
>Mature Secondary Structure
MRAKQRGVALIVILLLLAVMVSIAATMAERLFSQFQRATHQLNYQQAYWYSLGVEALAKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GIEQSYQDSETINLSQPWALKEQTYPLDYGQVRGKIRDMQACFNLNALAGVKLTPDSVKK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCEECHHHHCC
PYLLTVLQALLEGLEVESYQAEVIADSTLEFIDKDDSVRTAYGVEDSYYESMIPAYMAAD
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHH
TWLADASEWRAVQQVGGETMNKALPYVCALPTDQWRLNVNTLPAEQAALLAAMFSPTLSP
HHHHCHHHHHHHHHHCHHHHHHHCCEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
ESAKTLLEGRPFDGWASVDDFLAQSALTGVDNAVREEAKKYLSVDSHYFELDAQVLVDTS
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECHHHEECHH
RVRIRSLFYSNDKKTATVIRRRFGGISERVSDRSTE
HHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301