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The map label for this gene is rpoE [H]

Identifier: 147675349

GI number: 147675349

Start: 2202347

End: 2202919

Strand: Reverse

Name: rpoE [H]

Synonym: VC0395_A2045

Alternate gene names: 147675349

Gene position: 2202919-2202347 (Counterclockwise)

Preceding gene: 147673734

Following gene: 147673480

Centisome position: 72.85

GC content: 44.85

Gene sequence:

>573_bases
ATGAACGAGCAACTGACCGATCAAGTGTTGATTGAGCGAGTTCAGAATGGGGATAAGCAAGCATTTAACCTTTTGGTTTT
GAGATATCAAAATAAAGTGTGCAACCTTATTTCCCGGTATGTAAGCAATTCCGGTGATGTGGCTGATGTAGCGCAGGAAG
CGTTTATTAAAGCGTATCGAGCCATTCCAACCTTTCGTGGCGAGAGTGCGTTTTACACTTGGTTGTACCGTATTGCTGTT
AATACCGCGAAAAATCACATTGTTGCGCAAAGCCGACGCCCACCCGCAAGTGATGTTGATGCTGAAGAAGCTGAATTCTA
TGAAGGTGGTGACGCATTAAAAGAATTATCGAACCCGGAGAACATTACGTTGTCCAAAGAGTTGAAGAAGACTGTGTTCG
ATGCAATTGATGCGTTACCGGATGATTTAAAAACGGCAATGACGTTGCGCGAGCTTGATGGCTTGAGTTACGAGGAAATT
GCGGAAGTGATGGATTGCCCTGTTGGTACAGTGCGTTCACGAATTTTCCGCGCTCGTGAGGCGGTAGAGAAGAAAATCAA
ACCTCTTCTGTAA

Upstream 100 bases:

>100_bases
TAAAGGTGCTCATGCAAGTAGTGGTGTCAATATTACAATTCGCATAATGAGTACCCATATCTGAGAAGGTAGGGGAATAA
CAATAGGAGTGACCGCTCGA

Downstream 100 bases:

>100_bases
CGCAAATTCCGTAATGACTATGGTGAATAGAATGGCTGACAAAGAAAAACTTTCAGCACTCATGGATGGCGAGATGATCG
ACAATGAGCTGATTTTAGGA

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MNEQLTDQVLIERVQNGDKQAFNLLVLRYQNKVCNLISRYVSNSGDVADVAQEAFIKAYRAIPTFRGESAFYTWLYRIAV
NTAKNHIVAQSRRPPASDVDAEEAEFYEGGDALKELSNPENITLSKELKKTVFDAIDALPDDLKTAMTLRELDGLSYEEI
AEVMDCPVGTVRSRIFRAREAVEKKIKPLL

Sequences:

>Translated_190_residues
MNEQLTDQVLIERVQNGDKQAFNLLVLRYQNKVCNLISRYVSNSGDVADVAQEAFIKAYRAIPTFRGESAFYTWLYRIAV
NTAKNHIVAQSRRPPASDVDAEEAEFYEGGDALKELSNPENITLSKELKKTVFDAIDALPDDLKTAMTLRELDGLSYEEI
AEVMDCPVGTVRSRIFRAREAVEKKIKPLL
>Mature_190_residues
MNEQLTDQVLIERVQNGDKQAFNLLVLRYQNKVCNLISRYVSNSGDVADVAQEAFIKAYRAIPTFRGESAFYTWLYRIAV
NTAKNHIVAQSRRPPASDVDAEEAEFYEGGDALKELSNPENITLSKELKKTVFDAIDALPDDLKTAMTLRELDGLSYEEI
AEVMDCPVGTVRSRIFRAREAVEKKIKPLL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=190, Percent_Identity=73.6842105263158, Blast_Score=298, Evalue=1e-82,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21462; Mature: 21462

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQLTDQVLIERVQNGDKQAFNLLVLRYQNKVCNLISRYVSNSGDVADVAQEAFIKAYR
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AIPTFRGESAFYTWLYRIAVNTAKNHIVAQSRRPPASDVDAEEAEFYEGGDALKELSNPE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHCCCCC
NITLSKELKKTVFDAIDALPDDLKTAMTLRELDGLSYEEIAEVMDCPVGTVRSRIFRARE
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AVEKKIKPLL
HHHHHHCCCC
>Mature Secondary Structure
MNEQLTDQVLIERVQNGDKQAFNLLVLRYQNKVCNLISRYVSNSGDVADVAQEAFIKAYR
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AIPTFRGESAFYTWLYRIAVNTAKNHIVAQSRRPPASDVDAEEAEFYEGGDALKELSNPE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHCCCCC
NITLSKELKKTVFDAIDALPDDLKTAMTLRELDGLSYEEIAEVMDCPVGTVRSRIFRARE
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AVEKKIKPLL
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]