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The map label for this gene is vacB [H]

Identifier: 147675348

GI number: 147675348

Start: 2319883

End: 2322348

Strand: Reverse

Name: vacB [H]

Synonym: VC0395_A2177

Alternate gene names: 147675348

Gene position: 2322348-2319883 (Counterclockwise)

Preceding gene: 147674337

Following gene: 147673348

Centisome position: 76.8

GC content: 50.65

Gene sequence:

>2466_bases
ATGTCTGATACCACACATCTTGATCCTTTCGCTGATCGCGAAGCCGACAATTATGACAATCCAATTCCGAGTCGTGAGTA
CATTCTCGAATTTTTAACCCAAGCCAATGTTCCCATGAATCGCAACGATCTGTTCGAAGCCCTCAAACTAGAAGGCGAAG
AGCAATATGAAGGGTTGCGTCGTCGCCTGCGGGCAATGGAACGAGATGGCCAACTGGTGTTCACGCGCCGTCAATGCTAT
GCCTTGCCAGAAAAACTGGAAATGGTGAAAGGCTATGTGATTGGCCACAAAGACGGCCACGGCTGGGTTCGTCCAGAAGG
AAGCTTAAACAAAGAAGGCGATATTTTATTGCCCCACCACCAGATGCGTACGCTGATCCATGGTGATTTTGTCTTAGTGC
AGCCGAGCGGTACTGATAAACGAGGCCGTAAAGAAGGGCGCTTAGTACGCATTCTCGAAGAGCGCAATGGCCAAATTGTC
GGGCGTTTCTTCTTTGAATACGGTTACTCTTATGTGGTGCCGGATGACTCACGCATTCATCATGACATTCTGATCCCCAA
TGACTTACGTGCCGGCGCTCGCATGGGCAACGTGGTCGTGATTGAAATTACCGACCGTGGCACGCGTAACCGCGGCATGA
TGGGTAAAGTCGTTGAAGTGTTGGGTGAAAACATGGCCCCGGGTATGGAAACGCAAATTGCGATTCGTACTCACCAAATT
CCCCATGAATGGCCCGCAGAAGTTGAGCAGCAAGTAGCGGGATTGACCGAGGAAGTACCTGAAGAAGCCAAGCAAGGCCG
TGTCGATCTACGTGCTTTGCCATTAGTCACGATCGATGGCGAAGATGCTCGAGATTTCGATGATGCTGTCTATTGTGAAG
CGAAAAAAGGCGGTGGCTGGCGTTTATGGGTCGCGATTGCCGACGTGAGCTATTACGTACGTCCCGATACCGCTCTCGAT
AAAGAAGCGATCAACCGCGGTAACTCGGTCTATTTCCCATCGCAAGTTGTCCCAATGTTGCCGGAAGTGCTGTCGAACGG
CCTCTGTTCACTCAACCCACAAGTCGATCGCTTGTGCATGGTGTGTGAAATGACCGTATCGGAAACGGGTAAGCTCTCGG
GCTACAAGCATTACGAAGCGGTAATGAACTCGCACGCTCGCCTGACTTACACCAAAGTGCATGAAATCCTCGAAGGGGAT
GAAGAACTGCGCGAGCGTTATAAAGCGCTGGTTCCTCATCTTGAAGAGTTGCACAAAATGTATCAGGTGCTGAAAAGTGC
ACGCGATGAGCGTGGCGCGATTGAGTTTGAAACCGTTGAAACCAAATTCATCTTCAACGCTCAGCGCAAGATTGAAAGCA
TTGAGCCTGTTGTACGTAACGATGCGCATAAGCTGATTGAAGAGTGTATGATCCTCGCCAACATCGCCTCTGCCTCTTTG
GTAGAAAAAGCCAAAGAAGCGGCGTTGTACCGTGTCCATGAGCCACCAGGTGAAGAACGTCTCACTGGCTTCCGTGATTT
CCTCGGTGAGTTGGGGCTCGATCTCTCCGGTGGCCTTGAGCCATCACCGACCGACTACGCCAATTTAATGAAGCAAATTG
GTGAACGTCCAGATAAAGAGCTGATCCAAACCATGTTGCTGCGCTCGATGAAGCAAGCGGTGTATAACGCGGATAACGCG
GGTCACTTTGGCTTGGCGTTGAAACGTTACGCACACTTTACATCGCCAATCCGTCGTTACCCGGATTTGTTGCTGCATCG
TGCGATCAAATATCTGATCGCGAAGCAAGAAGGGCGCAACCAAGACCGTTGGACTCCAACCGGTGGCTATCACTACTCCT
TCGATGACATGGACTTCTACGGTGAGCAGTGCTCCATGACTGAGCGCCGCGCTGATGATGCGACGCGCGAAGTGTCTGAC
TGGCTCAAATGCGAGTATATGCAAGATCATGTGGGTGAAGAATTAGAAGGCGTCGTCGCGAACGTCACCAGTTTCGGCTT
CTTTGTCCGTCTCACTGAGCTGCACATTGACGGCCTGGTACACATTTCGACTCTGGCTAACGACTACTACCACTACGATC
CGATTGGTCAGCGTCTGGTGGGGGAAAGTTTTGGTGCCATCTATCGTCTTGGTGATGCGGTAAAAGTAAAAGTGTTGGCG
GTAAACCTTGATGATCGCCAAATTGACTTTGAATTGGTCGAAACTAGTCGTAAGCTACGCGGCCAAGGTAAAACCGCCAA
GAAACGTGCCGATGAAGCTCGAGCCAAAGCGCAAGGTAAAAAAGAAGCGGCCACTAAAGGTGCCCGTGGCAAATCACCAA
CGAAATCAGAGTTGAAACCACAGGTAGAAGCGACGCGCCGTCCAGACAGTGAAGGCCGTAGCAAACCGAAAAAGACCAAA
GCGCCGAAAAAACGCAAAGACCAAGCTCGTAAGAAAAGCGGCAAAGTACGAGATAAAACCAAATAG

Upstream 100 bases:

>100_bases
ACCTAGAATGATGAAAGAGTTCTGTGCGTTGTCATTAGTTGTTCGTAGTGACAAAGCAGTAGGATATAACTAACACCTTC
CTAGATAAGCAGGTCTGCCT

Downstream 100 bases:

>100_bases
GTAACACATGAGTAATGAATTGATTTATGGCATCCACGCCGTGAACGCGGTACTACAACGCGATCCCGCGCGTTTTGTCG
AAGCGTATGTGCTTAAAGGT

Product: ribonuclease R

Products: NA

Alternate protein names: RNase R [H]

Number of amino acids: Translated: 821; Mature: 820

Protein sequence:

>821_residues
MSDTTHLDPFADREADNYDNPIPSREYILEFLTQANVPMNRNDLFEALKLEGEEQYEGLRRRLRAMERDGQLVFTRRQCY
ALPEKLEMVKGYVIGHKDGHGWVRPEGSLNKEGDILLPHHQMRTLIHGDFVLVQPSGTDKRGRKEGRLVRILEERNGQIV
GRFFFEYGYSYVVPDDSRIHHDILIPNDLRAGARMGNVVVIEITDRGTRNRGMMGKVVEVLGENMAPGMETQIAIRTHQI
PHEWPAEVEQQVAGLTEEVPEEAKQGRVDLRALPLVTIDGEDARDFDDAVYCEAKKGGGWRLWVAIADVSYYVRPDTALD
KEAINRGNSVYFPSQVVPMLPEVLSNGLCSLNPQVDRLCMVCEMTVSETGKLSGYKHYEAVMNSHARLTYTKVHEILEGD
EELRERYKALVPHLEELHKMYQVLKSARDERGAIEFETVETKFIFNAQRKIESIEPVVRNDAHKLIEECMILANIASASL
VEKAKEAALYRVHEPPGEERLTGFRDFLGELGLDLSGGLEPSPTDYANLMKQIGERPDKELIQTMLLRSMKQAVYNADNA
GHFGLALKRYAHFTSPIRRYPDLLLHRAIKYLIAKQEGRNQDRWTPTGGYHYSFDDMDFYGEQCSMTERRADDATREVSD
WLKCEYMQDHVGEELEGVVANVTSFGFFVRLTELHIDGLVHISTLANDYYHYDPIGQRLVGESFGAIYRLGDAVKVKVLA
VNLDDRQIDFELVETSRKLRGQGKTAKKRADEARAKAQGKKEAATKGARGKSPTKSELKPQVEATRRPDSEGRSKPKKTK
APKKRKDQARKKSGKVRDKTK

Sequences:

>Translated_821_residues
MSDTTHLDPFADREADNYDNPIPSREYILEFLTQANVPMNRNDLFEALKLEGEEQYEGLRRRLRAMERDGQLVFTRRQCY
ALPEKLEMVKGYVIGHKDGHGWVRPEGSLNKEGDILLPHHQMRTLIHGDFVLVQPSGTDKRGRKEGRLVRILEERNGQIV
GRFFFEYGYSYVVPDDSRIHHDILIPNDLRAGARMGNVVVIEITDRGTRNRGMMGKVVEVLGENMAPGMETQIAIRTHQI
PHEWPAEVEQQVAGLTEEVPEEAKQGRVDLRALPLVTIDGEDARDFDDAVYCEAKKGGGWRLWVAIADVSYYVRPDTALD
KEAINRGNSVYFPSQVVPMLPEVLSNGLCSLNPQVDRLCMVCEMTVSETGKLSGYKHYEAVMNSHARLTYTKVHEILEGD
EELRERYKALVPHLEELHKMYQVLKSARDERGAIEFETVETKFIFNAQRKIESIEPVVRNDAHKLIEECMILANIASASL
VEKAKEAALYRVHEPPGEERLTGFRDFLGELGLDLSGGLEPSPTDYANLMKQIGERPDKELIQTMLLRSMKQAVYNADNA
GHFGLALKRYAHFTSPIRRYPDLLLHRAIKYLIAKQEGRNQDRWTPTGGYHYSFDDMDFYGEQCSMTERRADDATREVSD
WLKCEYMQDHVGEELEGVVANVTSFGFFVRLTELHIDGLVHISTLANDYYHYDPIGQRLVGESFGAIYRLGDAVKVKVLA
VNLDDRQIDFELVETSRKLRGQGKTAKKRADEARAKAQGKKEAATKGARGKSPTKSELKPQVEATRRPDSEGRSKPKKTK
APKKRKDQARKKSGKVRDKTK
>Mature_820_residues
SDTTHLDPFADREADNYDNPIPSREYILEFLTQANVPMNRNDLFEALKLEGEEQYEGLRRRLRAMERDGQLVFTRRQCYA
LPEKLEMVKGYVIGHKDGHGWVRPEGSLNKEGDILLPHHQMRTLIHGDFVLVQPSGTDKRGRKEGRLVRILEERNGQIVG
RFFFEYGYSYVVPDDSRIHHDILIPNDLRAGARMGNVVVIEITDRGTRNRGMMGKVVEVLGENMAPGMETQIAIRTHQIP
HEWPAEVEQQVAGLTEEVPEEAKQGRVDLRALPLVTIDGEDARDFDDAVYCEAKKGGGWRLWVAIADVSYYVRPDTALDK
EAINRGNSVYFPSQVVPMLPEVLSNGLCSLNPQVDRLCMVCEMTVSETGKLSGYKHYEAVMNSHARLTYTKVHEILEGDE
ELRERYKALVPHLEELHKMYQVLKSARDERGAIEFETVETKFIFNAQRKIESIEPVVRNDAHKLIEECMILANIASASLV
EKAKEAALYRVHEPPGEERLTGFRDFLGELGLDLSGGLEPSPTDYANLMKQIGERPDKELIQTMLLRSMKQAVYNADNAG
HFGLALKRYAHFTSPIRRYPDLLLHRAIKYLIAKQEGRNQDRWTPTGGYHYSFDDMDFYGEQCSMTERRADDATREVSDW
LKCEYMQDHVGEELEGVVANVTSFGFFVRLTELHIDGLVHISTLANDYYHYDPIGQRLVGESFGAIYRLGDAVKVKVLAV
NLDDRQIDFELVETSRKLRGQGKTAKKRADEARAKAQGKKEAATKGARGKSPTKSELKPQVEATRRPDSEGRSKPKKTKA
PKKRKDQARKKSGKVRDKTK

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI134288890, Length=593, Percent_Identity=26.8128161888702, Blast_Score=179, Evalue=1e-44,
Organism=Homo sapiens, GI190014623, Length=426, Percent_Identity=30.7511737089202, Blast_Score=176, Evalue=1e-43,
Organism=Homo sapiens, GI190014625, Length=426, Percent_Identity=30.7511737089202, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI19115966, Length=531, Percent_Identity=25.8003766478343, Blast_Score=155, Evalue=2e-37,
Organism=Homo sapiens, GI219521928, Length=531, Percent_Identity=25.8003766478343, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI87082383, Length=741, Percent_Identity=67.2064777327935, Blast_Score=1046, Evalue=0.0,
Organism=Escherichia coli, GI1787542, Length=652, Percent_Identity=23.7730061349693, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17553506, Length=557, Percent_Identity=28.5457809694794, Blast_Score=194, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI212645896, Length=417, Percent_Identity=31.89448441247, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324552, Length=526, Percent_Identity=26.9961977186312, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320499, Length=425, Percent_Identity=21.8823529411765, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24649634, Length=471, Percent_Identity=29.9363057324841, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI19922976, Length=375, Percent_Identity=28.5333333333333, Blast_Score=135, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24654597, Length=375, Percent_Identity=28.5333333333333, Blast_Score=135, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24654592, Length=375, Percent_Identity=28.5333333333333, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- InterPro:   IPR013668 [H]

Pfam domain/function: PF08461 HTH_12; PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 93388; Mature: 93257

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTHLDPFADREADNYDNPIPSREYILEFLTQANVPMNRNDLFEALKLEGEEQYEGLR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHH
RRLRAMERDGQLVFTRRQCYALPEKLEMVKGYVIGHKDGHGWVRPEGSLNKEGDILLPHH
HHHHHHHCCCCEEEEHHHHHHCHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCEECCHH
QMRTLIHGDFVLVQPSGTDKRGRKEGRLVRILEERNGQIVGRFFFEYGYSYVVPDDSRIH
HHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHCCCEEECCCCCCCC
HDILIPNDLRAGARMGNVVVIEITDRGTRNRGMMGKVVEVLGENMAPGMETQIAIRTHQI
EEEECCCCHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCC
PHEWPAEVEQQVAGLTEEVPEEAKQGRVDLRALPLVTIDGEDARDFDDAVYCEAKKGGGW
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCE
RLWVAIADVSYYVRPDTALDKEAINRGNSVYFPSQVVPMLPEVLSNGLCSLNPQVDRLCM
EEEEEEECCEEEECCCCCCCHHHHCCCCCEECCHHHHHHHHHHHCCCCCCCCCHHHHHHH
VCEMTVSETGKLSGYKHYEAVMNSHARLTYTKVHEILEGDEELRERYKALVPHLEELHKM
HHHHHHHCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YQVLKSARDERGAIEFETVETKFIFNAQRKIESIEPVVRNDAHKLIEECMILANIASASL
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEKAKEAALYRVHEPPGEERLTGFRDFLGELGLDLSGGLEPSPTDYANLMKQIGERPDKE
HHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
LIQTMLLRSMKQAVYNADNAGHFGLALKRYAHFTSPIRRYPDLLLHRAIKYLIAKQEGRN
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QDRWTPTGGYHYSFDDMDFYGEQCSMTERRADDATREVSDWLKCEYMQDHVGEELEGVVA
CCCCCCCCCEEECCCCCHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
NVTSFGFFVRLTELHIDGLVHISTLANDYYHYDPIGQRLVGESFGAIYRLGDAVKVKVLA
HHHHHHHHEEEHHHHHCCCEEHHHHHCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEEEE
VNLDDRQIDFELVETSRKLRGQGKTAKKRADEARAKAQGKKEAATKGARGKSPTKSELKP
EECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHCCC
QVEATRRPDSEGRSKPKKTKAPKKRKDQARKKSGKVRDKTK
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDTTHLDPFADREADNYDNPIPSREYILEFLTQANVPMNRNDLFEALKLEGEEQYEGLR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHH
RRLRAMERDGQLVFTRRQCYALPEKLEMVKGYVIGHKDGHGWVRPEGSLNKEGDILLPHH
HHHHHHHCCCCEEEEHHHHHHCHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCEECCHH
QMRTLIHGDFVLVQPSGTDKRGRKEGRLVRILEERNGQIVGRFFFEYGYSYVVPDDSRIH
HHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHCCCEEECCCCCCCC
HDILIPNDLRAGARMGNVVVIEITDRGTRNRGMMGKVVEVLGENMAPGMETQIAIRTHQI
EEEECCCCHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCC
PHEWPAEVEQQVAGLTEEVPEEAKQGRVDLRALPLVTIDGEDARDFDDAVYCEAKKGGGW
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCE
RLWVAIADVSYYVRPDTALDKEAINRGNSVYFPSQVVPMLPEVLSNGLCSLNPQVDRLCM
EEEEEEECCEEEECCCCCCCHHHHCCCCCEECCHHHHHHHHHHHCCCCCCCCCHHHHHHH
VCEMTVSETGKLSGYKHYEAVMNSHARLTYTKVHEILEGDEELRERYKALVPHLEELHKM
HHHHHHHCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YQVLKSARDERGAIEFETVETKFIFNAQRKIESIEPVVRNDAHKLIEECMILANIASASL
HHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEKAKEAALYRVHEPPGEERLTGFRDFLGELGLDLSGGLEPSPTDYANLMKQIGERPDKE
HHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
LIQTMLLRSMKQAVYNADNAGHFGLALKRYAHFTSPIRRYPDLLLHRAIKYLIAKQEGRN
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QDRWTPTGGYHYSFDDMDFYGEQCSMTERRADDATREVSDWLKCEYMQDHVGEELEGVVA
CCCCCCCCCEEECCCCCHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
NVTSFGFFVRLTELHIDGLVHISTLANDYYHYDPIGQRLVGESFGAIYRLGDAVKVKVLA
HHHHHHHHEEEHHHHHCCCEEHHHHHCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEEEE
VNLDDRQIDFELVETSRKLRGQGKTAKKRADEARAKAQGKKEAATKGARGKSPTKSELKP
EECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHCCC
QVEATRRPDSEGRSKPKKTKAPKKRKDQARKKSGKVRDKTK
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]