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The map label for this gene is rlmB [H]

Identifier: 147673348

GI number: 147673348

Start: 2319132

End: 2319875

Strand: Reverse

Name: rlmB [H]

Synonym: VC0395_A2176

Alternate gene names: 147673348

Gene position: 2319875-2319132 (Counterclockwise)

Preceding gene: 147675348

Following gene: 147675533

Centisome position: 76.71

GC content: 52.55

Gene sequence:

>744_bases
ATGAGTAATGAATTGATTTATGGCATCCACGCCGTGAACGCGGTACTACAACGCGATCCCGCGCGTTTTGTCGAAGCGTA
TGTGCTTAAAGGTCGTCAGGACGATCGCCTGCTGCCGGTACTCAACGAATTGATGCGTCTTGGCGTCTCAATTCAAGAGA
TGAGCCGCAAAGCGCTGGATGACAAAGCGGAAGGCGCCAATCATCAAGGATTGATTGCCCGTGTTAAACCTGCTCGTCAA
CTCAATGAAAATGACCTTGACGATATCCTCGCTCAGCATGAGCAACCCCTGTTGTTAGTACTGGATGGGGTAACTGATCC
GCACAACCTCGGGGCTTGCCTGCGTAATGCCGATGCGGCAGGTGTGGCGGCAGTCATTGTTCCGAAAGACAAATCAGCAC
CACTGACCGCGACCGTCAGTAAAGTGGCGTGTGGCGCGGCGGAAACGGTACCGCTCGTGCGCGTTACTAACTTGGCGCGT
ACCATGCGCGCACTGCAAGAAAAAGGTGTCTGGTTTGTAGGGACTGCAGGCGAAGCGACTCATGATATCTATCAAAGCAA
ACTGACCGGGCCGCTGGCGATTGTAATGGGCGCAGAAGGGGATGGCATGCGTCGTTTAACGCGTGAAACCTGTGATGATT
TGATTAAAATCCCGATGGCAGGCAGCGTATCAAGTCTAAACGTGTCTGTAGCTTGTGGTGTCTGTTTATTTGAAGCAGTA
CGCCAGCGTATGGCGAATCGGTAA

Upstream 100 bases:

>100_bases
GGCCGTAGCAAACCGAAAAAGACCAAAGCGCCGAAAAAACGCAAAGACCAAGCTCGTAAGAAAAGCGGCAAAGTACGAGA
TAAAACCAAATAGGTAACAC

Downstream 100 bases:

>100_bases
GTTCAATCATTCCATCATCGTAAACGGGCACTTGAGAGTGCCCGTTTTTCTTATCATCCCTAAACCACGGCATTTAGCGG
TCGTGATTGTTTGGCGCATG

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSNELIYGIHAVNAVLQRDPARFVEAYVLKGRQDDRLLPVLNELMRLGVSIQEMSRKALDDKAEGANHQGLIARVKPARQ
LNENDLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAAGVAAVIVPKDKSAPLTATVSKVACGAAETVPLVRVTNLAR
TMRALQEKGVWFVGTAGEATHDIYQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVACGVCLFEAV
RQRMANR

Sequences:

>Translated_247_residues
MSNELIYGIHAVNAVLQRDPARFVEAYVLKGRQDDRLLPVLNELMRLGVSIQEMSRKALDDKAEGANHQGLIARVKPARQ
LNENDLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAAGVAAVIVPKDKSAPLTATVSKVACGAAETVPLVRVTNLAR
TMRALQEKGVWFVGTAGEATHDIYQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVACGVCLFEAV
RQRMANR
>Mature_246_residues
SNELIYGIHAVNAVLQRDPARFVEAYVLKGRQDDRLLPVLNELMRLGVSIQEMSRKALDDKAEGANHQGLIARVKPARQL
NENDLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAAGVAAVIVPKDKSAPLTATVSKVACGAAETVPLVRVTNLART
MRALQEKGVWFVGTAGEATHDIYQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVACGVCLFEAVR
QRMANR

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=267, Percent_Identity=30.7116104868914, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=71.7842323651452, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1788935, Length=245, Percent_Identity=26.9387755102041, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790083, Length=159, Percent_Identity=32.0754716981132, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=264, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=286, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26612; Mature: 26481

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNELIYGIHAVNAVLQRDPARFVEAYVLKGRQDDRLLPVLNELMRLGVSIQEMSRKALD
CCCCEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHC
DKAEGANHQGLIARVKPARQLNENDLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAA
HHHCCCCCCCEEEEECCHHHCCCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCC
GVAAVIVPKDKSAPLTATVSKVACGAAETVPLVRVTNLARTMRALQEKGVWFVGTAGEAT
CEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
HDIYQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVACGVCLFEAV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RQRMANR
HHHHCCC
>Mature Secondary Structure 
SNELIYGIHAVNAVLQRDPARFVEAYVLKGRQDDRLLPVLNELMRLGVSIQEMSRKALD
CCCEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHC
DKAEGANHQGLIARVKPARQLNENDLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAA
HHHCCCCCCCEEEEECCHHHCCCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCC
GVAAVIVPKDKSAPLTATVSKVACGAAETVPLVRVTNLARTMRALQEKGVWFVGTAGEAT
CEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
HDIYQSKLTGPLAIVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVACGVCLFEAV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RQRMANR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]