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The map label for this gene is bioF [H]

Identifier: 147675336

GI number: 147675336

Start: 673731

End: 674885

Strand: Direct

Name: bioF [H]

Synonym: VC0395_A0631

Alternate gene names: 147675336

Gene position: 673731-674885 (Clockwise)

Preceding gene: 147673516

Following gene: 147673959

Centisome position: 22.28

GC content: 52.55

Gene sequence:

>1155_bases
ATGACGCTGCGCTTTAAGTCGCGGATTGAGCAAGCTCTGAATGAGCGTGAACATTCAGGGCTGACTCGCCGTCTTCAGCT
TTTAGATCGAAGCGCACAAGGTGAACTGATTCAGCAAGATCGTACTTACCTGAACTTCTCAAGCAACGATTACCTTGGTT
TAGCCAATGATGCTGAGTTAGTGCAGGCTTGGCAAACCGGTCTGGCGCGCTTTGGCGCAGGCAGTGGCGCTTCGCCGATG
GTAACTGGTTTTAGCTCCGCTCATGCCGAGTTAGAGCATGAGTTGTGTCACTGGCTTGGTTACGAGCGAGCAGTCTTGTT
CAGCTCTGGCTTTAGTGCCAATCAAGCCCTGCTGTTTACTTTGCTCGAAAAAGAGGACTTGCTGCTGCAAGACAAACTCA
ATCACGCCTCCTTGATGGAAGCGGGTATGTTAAGTCCTGCCACCATGAAACGCTTTAAGCACAATGATACTGAGCATCTT
CGCCAATTACTGCATGAGCAGAGCAACAGCTTAGTGGTGACGGAAGGGGTGTTTAGCATGGATGGCGATTGCGCACCCTT
GGCGCAAATTGCAACTTTAGTGAAACAGCGCGCTTGGTTGATGGTGGATGATGCGCACGGCATTGGCGTGCTTGGAGAGG
ATGGGGCAGGGAGCTGCCAAGCGGCAGGGATTCATCCCGAGATTCTGGTCGTTACTTTTGGTAAAGCTTTTGGGTTAGCA
GGTGCGGCGGTGTTGTGTGATGCGCACGTTGGTGATTATCTGACGCAATTTGCACGCCACCATGTGTATTCCACCGCTAT
GCCTCCGGCACAAGCGCATGCTCTGACTCATGCTGCGCGGATGATCCAAAGTCAGTCTTGGCGCAGAGAACAATTGGCTG
AGCTGCTGGCGTGTTTTGATGAGCAGTGCCGCAATATTCCGGGTTTTGTGGCAACACAAACGCCCATCAAACCTTGGTTG
TTGGGCAGCTCTGATTCCGCATTGAGTGCATCCCACGAGCTCAAGCAGCAAGGGATTTGGGTGAGTGCCATTCGACCACC
CACCGTGCCTGTAGGCAGTGCGCGTTTACGTATCACGATAACGGCGGCGCATACCCAAGCGCAGATACGCCGCTTATCGG
AGCAGTTAAAACATGTGATGGAGAAGGACACATGA

Upstream 100 bases:

>100_bases
GAACAAGTGGCGCAAAAGCCCGATGAAATCACGGAAAATGAGTTGTTGGATAGAGTCGTTGAACGAGTAGCCGCGCGCCC
AACCGCCAGCGATTTATTTT

Downstream 100 bases:

>100_bases
GCATGACAGCCACGGAGCTTTGTGTTGAATTAAAAGACAAAAGTGCGATTGCGCAAGCCTTTGGCAAAGCCGCCGCGCAC
TATGATCAGCACGCGGCTTT

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTLRFKSRIEQALNEREHSGLTRRLQLLDRSAQGELIQQDRTYLNFSSNDYLGLANDAELVQAWQTGLARFGAGSGASPM
VTGFSSAHAELEHELCHWLGYERAVLFSSGFSANQALLFTLLEKEDLLLQDKLNHASLMEAGMLSPATMKRFKHNDTEHL
RQLLHEQSNSLVVTEGVFSMDGDCAPLAQIATLVKQRAWLMVDDAHGIGVLGEDGAGSCQAAGIHPEILVVTFGKAFGLA
GAAVLCDAHVGDYLTQFARHHVYSTAMPPAQAHALTHAARMIQSQSWRREQLAELLACFDEQCRNIPGFVATQTPIKPWL
LGSSDSALSASHELKQQGIWVSAIRPPTVPVGSARLRITITAAHTQAQIRRLSEQLKHVMEKDT

Sequences:

>Translated_384_residues
MTLRFKSRIEQALNEREHSGLTRRLQLLDRSAQGELIQQDRTYLNFSSNDYLGLANDAELVQAWQTGLARFGAGSGASPM
VTGFSSAHAELEHELCHWLGYERAVLFSSGFSANQALLFTLLEKEDLLLQDKLNHASLMEAGMLSPATMKRFKHNDTEHL
RQLLHEQSNSLVVTEGVFSMDGDCAPLAQIATLVKQRAWLMVDDAHGIGVLGEDGAGSCQAAGIHPEILVVTFGKAFGLA
GAAVLCDAHVGDYLTQFARHHVYSTAMPPAQAHALTHAARMIQSQSWRREQLAELLACFDEQCRNIPGFVATQTPIKPWL
LGSSDSALSASHELKQQGIWVSAIRPPTVPVGSARLRITITAAHTQAQIRRLSEQLKHVMEKDT
>Mature_383_residues
TLRFKSRIEQALNEREHSGLTRRLQLLDRSAQGELIQQDRTYLNFSSNDYLGLANDAELVQAWQTGLARFGAGSGASPMV
TGFSSAHAELEHELCHWLGYERAVLFSSGFSANQALLFTLLEKEDLLLQDKLNHASLMEAGMLSPATMKRFKHNDTEHLR
QLLHEQSNSLVVTEGVFSMDGDCAPLAQIATLVKQRAWLMVDDAHGIGVLGEDGAGSCQAAGIHPEILVVTFGKAFGLAG
AAVLCDAHVGDYLTQFARHHVYSTAMPPAQAHALTHAARMIQSQSWRREQLAELLACFDEQCRNIPGFVATQTPIKPWLL
GSSDSALSASHELKQQGIWVSAIRPPTVPVGSARLRITITAAHTQAQIRRLSEQLKHVMEKDT

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=361, Percent_Identity=32.9639889196676, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI284448556, Length=345, Percent_Identity=33.0434782608696, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI4758668, Length=349, Percent_Identity=30.6590257879656, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI83977444, Length=351, Percent_Identity=33.048433048433, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI83977440, Length=351, Percent_Identity=33.048433048433, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI83977442, Length=349, Percent_Identity=33.5243553008596, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI119220554, Length=359, Percent_Identity=30.08356545961, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4502025, Length=397, Percent_Identity=28.9672544080605, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI40316939, Length=397, Percent_Identity=28.9672544080605, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI5454084, Length=374, Percent_Identity=21.9251336898396, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786993, Length=347, Percent_Identity=54.178674351585, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI1790046, Length=392, Percent_Identity=34.1836734693878, Blast_Score=204, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI71994529, Length=362, Percent_Identity=33.1491712707182, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17560912, Length=359, Percent_Identity=29.2479108635098, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17560914, Length=359, Percent_Identity=29.2479108635098, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32566772, Length=368, Percent_Identity=27.1739130434783, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71982625, Length=270, Percent_Identity=23.7037037037037, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71982617, Length=284, Percent_Identity=23.943661971831, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320267, Length=410, Percent_Identity=28.2926829268293, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6320438, Length=356, Percent_Identity=32.3033707865169, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323954, Length=271, Percent_Identity=25.830258302583, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24662918, Length=340, Percent_Identity=34.4117647058824, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17137420, Length=346, Percent_Identity=31.2138728323699, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17136286, Length=371, Percent_Identity=26.9541778975741, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24653280, Length=286, Percent_Identity=26.2237762237762, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653276, Length=286, Percent_Identity=26.2237762237762, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653278, Length=286, Percent_Identity=26.2237762237762, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 42178; Mature: 42047

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRFKSRIEQALNEREHSGLTRRLQLLDRSAQGELIQQDRTYLNFSSNDYLGLANDAEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEECCCCCEECCCHHHH
VQAWQTGLARFGAGSGASPMVTGFSSAHAELEHELCHWLGYERAVLFSSGFSANQALLFT
HHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
LLEKEDLLLQDKLNHASLMEAGMLSPATMKRFKHNDTEHLRQLLHEQSNSLVVTEGVFSM
HHCCHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCC
DGDCAPLAQIATLVKQRAWLMVDDAHGIGVLGEDGAGSCQAAGIHPEILVVTFGKAFGLA
CCCCCHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCEECCCCCEEEEEEECHHHHHC
GAAVLCDAHVGDYLTQFARHHVYSTAMPPAQAHALTHAARMIQSQSWRREQLAELLACFD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EQCRNIPGFVATQTPIKPWLLGSSDSALSASHELKQQGIWVSAIRPPTVPVGSARLRITI
HHHCCCCCCEEECCCCCCEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEE
TAAHTQAQIRRLSEQLKHVMEKDT
EEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLRFKSRIEQALNEREHSGLTRRLQLLDRSAQGELIQQDRTYLNFSSNDYLGLANDAEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEECCCCCEECCCHHHH
VQAWQTGLARFGAGSGASPMVTGFSSAHAELEHELCHWLGYERAVLFSSGFSANQALLFT
HHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
LLEKEDLLLQDKLNHASLMEAGMLSPATMKRFKHNDTEHLRQLLHEQSNSLVVTEGVFSM
HHCCHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCC
DGDCAPLAQIATLVKQRAWLMVDDAHGIGVLGEDGAGSCQAAGIHPEILVVTFGKAFGLA
CCCCCHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCEECCCCCEEEEEEECHHHHHC
GAAVLCDAHVGDYLTQFARHHVYSTAMPPAQAHALTHAARMIQSQSWRREQLAELLACFD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EQCRNIPGFVATQTPIKPWLLGSSDSALSASHELKQQGIWVSAIRPPTVPVGSARLRITI
HHHCCCCCCEEECCCCCCEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEE
TAAHTQAQIRRLSEQLKHVMEKDT
EEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]