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The map label for this gene is bioB

Identifier: 147673516

GI number: 147673516

Start: 672695

End: 673747

Strand: Direct

Name: bioB

Synonym: VC0395_A0630

Alternate gene names: 147673516

Gene position: 672695-673747 (Clockwise)

Preceding gene: 147674801

Following gene: 147675336

Centisome position: 22.24

GC content: 51.09

Gene sequence:

>1053_bases
GTGGAAGTTCGTCATAACTGGACGGTCGCTGAGGTCAAAGCGTTACTGGATAAACCTTTTATGGATCTGCTGTTTGAAGC
GCAGCAGGTGCATCGTCTTCATCATCCGCACAACCATGTGCAGGTCAGCACGCTGCTGTCGATCAAAACTGGCGCTTGTC
CAGAAGATTGCAAATATTGCCCGCAAAGTGCTCACTACCGTACTGATGTAGATAAAGAGCGTTTGATGGAAGTCGAGCGC
GTATTGGATGCGGCGCAAAAAGCCAAAAATTCAGGCTCGACCCGTTTTTGTATGGGCGCGGCGTGGAAGAATCCGAAAGA
GCGTGATATGCCGCTGCTCAAAGAGATGATCCGCGGCGTGAAGGATATGGGGCTAGAAACCTGCATGACGCTGGGTATGT
TGACGCCAGATCAAGCGCAGCAATTGGCGCAAGCGGGGCTGGATTATTACAACCACAACCTTGATACCTCTCCGGAATTT
TATGGCAACATCATCACCACACGCACATACCAAGATCGCCTAGATACCTTATCGCACGTGCGTGATGCCGGGATGAAGAT
CTGCTCGGGGGGCATTATCGGCATGGGCGAAAGCACCAATGACCGCGCTGGGTTATTGGTTGAGCTCGCGAATTTGCCGA
CTCATCCGGAAAGTGTGCCGATCAACATGCTGGTCAAAGTGAAAGGTACACCACTGGAACAAGTCGATGATGTGGAACCC
TTTGATTTTGTGCGTTTGATTGCGGTAGCGCGCATTATGATGCCGAAATCTGCGGTTCGCCTTTCAGCTGGACGCGAGAA
GATGAATGAGCAGATGCAGGCGCTGTGCTTTATGGCAGGAGCCAATTCGATTTTCTACGGTTGCAAACTGCTGACCACCC
CCAACCCAGCGGAAGACAGCGATATGCTGCTGTTTAAAAAGCTAGGAATTAACCGCGAACAAGTGGCGCAAAAGCCCGAT
GAAATCACGGAAAATGAGTTGTTGGATAGAGTCGTTGAACGAGTAGCCGCGCGCCCAACCGCCAGCGATTTATTTTATGA
CGCTGCGCTTTAA

Upstream 100 bases:

>100_bases
TAACCACATGTAAACCTTGTGATTTTCTTATTGTTGACAGTCTAACGCCTGTCAGTAGACTAGCCAAGCATAAAACCAAT
AACCCCAATAAGGATTACAC

Downstream 100 bases:

>100_bases
GTCGCGGATTGAGCAAGCTCTGAATGAGCGTGAACATTCAGGGCTGACTCGCCGTCTTCAGCTTTTAGATCGAAGCGCAC
AAGGTGAACTGATTCAGCAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MEVRHNWTVAEVKALLDKPFMDLLFEAQQVHRLHHPHNHVQVSTLLSIKTGACPEDCKYCPQSAHYRTDVDKERLMEVER
VLDAAQKAKNSGSTRFCMGAAWKNPKERDMPLLKEMIRGVKDMGLETCMTLGMLTPDQAQQLAQAGLDYYNHNLDTSPEF
YGNIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESTNDRAGLLVELANLPTHPESVPINMLVKVKGTPLEQVDDVEP
FDFVRLIAVARIMMPKSAVRLSAGREKMNEQMQALCFMAGANSIFYGCKLLTTPNPAEDSDMLLFKKLGINREQVAQKPD
EITENELLDRVVERVAARPTASDLFYDAAL

Sequences:

>Translated_350_residues
MEVRHNWTVAEVKALLDKPFMDLLFEAQQVHRLHHPHNHVQVSTLLSIKTGACPEDCKYCPQSAHYRTDVDKERLMEVER
VLDAAQKAKNSGSTRFCMGAAWKNPKERDMPLLKEMIRGVKDMGLETCMTLGMLTPDQAQQLAQAGLDYYNHNLDTSPEF
YGNIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESTNDRAGLLVELANLPTHPESVPINMLVKVKGTPLEQVDDVEP
FDFVRLIAVARIMMPKSAVRLSAGREKMNEQMQALCFMAGANSIFYGCKLLTTPNPAEDSDMLLFKKLGINREQVAQKPD
EITENELLDRVVERVAARPTASDLFYDAAL
>Mature_350_residues
MEVRHNWTVAEVKALLDKPFMDLLFEAQQVHRLHHPHNHVQVSTLLSIKTGACPEDCKYCPQSAHYRTDVDKERLMEVER
VLDAAQKAKNSGSTRFCMGAAWKNPKERDMPLLKEMIRGVKDMGLETCMTLGMLTPDQAQQLAQAGLDYYNHNLDTSPEF
YGNIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESTNDRAGLLVELANLPTHPESVPINMLVKVKGTPLEQVDDVEP
FDFVRLIAVARIMMPKSAVRLSAGREKMNEQMQALCFMAGANSIFYGCKLLTTPNPAEDSDMLLFKKLGINREQVAQKPD
EITENELLDRVVERVAARPTASDLFYDAAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=349, Percent_Identity=69.3409742120344, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=49.211356466877, Blast_Score=320, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_VIBC3 (A5F2H3)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F2H3
- SMR:   A5F2H3
- STRING:   A5F2H3
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0630
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2300362
- BioCyc:   VCHO345073:VC0395_A0630-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39238; Mature: 39238

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRHNWTVAEVKALLDKPFMDLLFEAQQVHRLHHPHNHVQVSTLLSIKTGACPEDCKYC
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEHHHHEEECCCCCCHHHHHC
PQSAHYRTDVDKERLMEVERVLDAAQKAKNSGSTRFCMGAAWKNPKERDMPLLKEMIRGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
KDMGLETCMTLGMLTPDQAQQLAQAGLDYYNHNLDTSPEFYGNIITTRTYQDRLDTLSHV
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECHHHHHHHHHHHH
RDAGMKICSGGIIGMGESTNDRAGLLVELANLPTHPESVPINMLVKVKGTPLEQVDDVEP
HHHHHHHHCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHCCCCH
FDFVRLIAVARIMMPKSAVRLSAGREKMNEQMQALCFMAGANSIFYGCKLLTTPNPAEDS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECEEEECCCCCCCCC
DMLLFKKLGINREQVAQKPDEITENELLDRVVERVAARPTASDLFYDAAL
HHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MEVRHNWTVAEVKALLDKPFMDLLFEAQQVHRLHHPHNHVQVSTLLSIKTGACPEDCKYC
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEHHHHEEECCCCCCHHHHHC
PQSAHYRTDVDKERLMEVERVLDAAQKAKNSGSTRFCMGAAWKNPKERDMPLLKEMIRGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
KDMGLETCMTLGMLTPDQAQQLAQAGLDYYNHNLDTSPEFYGNIITTRTYQDRLDTLSHV
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECHHHHHHHHHHHH
RDAGMKICSGGIIGMGESTNDRAGLLVELANLPTHPESVPINMLVKVKGTPLEQVDDVEP
HHHHHHHHCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHCCCCH
FDFVRLIAVARIMMPKSAVRLSAGREKMNEQMQALCFMAGANSIFYGCKLLTTPNPAEDS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECEEEECCCCCCCCC
DMLLFKKLGINREQVAQKPDEITENELLDRVVERVAARPTASDLFYDAAL
HHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA