| Definition | Vibrio cholerae O395 chromosome 2, complete sequence. |
|---|---|
| Accession | NC_009457 |
| Length | 3,024,069 |
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The map label for this gene is metE [H]
Identifier: 147675183
GI number: 147675183
Start: 1388244
End: 1390529
Strand: Reverse
Name: metE [H]
Synonym: VC0395_A1308
Alternate gene names: 147675183
Gene position: 1390529-1388244 (Counterclockwise)
Preceding gene: 147673780
Following gene: 147673760
Centisome position: 45.98
GC content: 50.26
Gene sequence:
>2286_bases ATGACAACGACACACATTCTTGGTTATCCCTGCATTGGCGAAAAACGCGAACTTAAATTTGCTCTTGAAAAATACTGGCG TGGTGAGATTGACCAAGCGGCTTTGAAACAGGTCGGTAGTCAAATTCGCCAACACAGCTGGGCATTGCAAAAAGAAGCTG GATTAGACTTTGTGACCGCGGGTGACTTTGCTTGGTACGACCACGTACTCACCACCACACTATTACTGGGCCATGTGCCT AAGCGCCATAGCCACGGTTTTCCTGATTTAGATACTTTGTTCCGTGTCGGTCGTGGTCAGTCACAAAATGCTTGTGGTTG TGGAACGGGTAGCGCGGCTTCTGATATGACCAAGTGGTTCAACACCAACTATCACTACATCGTTCCGGAGTTCAGCTCTC ACGATACGTTTAATGTGAGCTGGCCACAACTGTTTGAAGAGGTGAATGAAGCGCTGCAAGCGGGTCACGATGTAAAACCT GTGCTGCTTGGCCCAATCAGTTACCTCTATTTAGGCAAAGAAGTGGAAGAGGGTTTTGATCGGTTAACGTTGTTGCCGCG TCTATTGACCGCTTACCAAGCGATTCTGAGCAAGCTGGCGAAGCAGGGTGTGCAATGGGTACAAATCGATGAGCCTATTT TGGCGTTAGAGCTGGAACCCCGTTGGCAAGAAGCATTTAAACTGGCTTATCAGGTGATCCGCGGTGACGTCAAACTGCTG CTGACCACTTACTTTGATTCAGTGTTAGATACGCTTGATAAGATAGTCGAACTGCCTGTCGATGGTTTGCATGTGGATAT TTCGGCCGCTCCAGCGCAGCTCGAAACTATTGTGAACCGCTTGCCCTCCGATTGGGTGCTTTCTGCGGGGGTCATTAATG GCCGTAACGTTTGGCGTGCAGATTTGAGTGCCATTCTGGCTCGTCTACAACCTGTAAAAACCTTGCTGGGTGAGCGTTTG TGGGTCGCCAGTTCCTGTTCACTGCTGCACAGCCCTGTCGATCTGGATCTGGAAGGCGATCTGAGCGCAGAAACCCGCAG TTGGTTTGCGTTTGCCAAGCAGAAAGTGACCGAAGTCGCCTTGCTCGGCCGTGCTCTGGAGGGGGATGCCGCCGCAATTT TAGCGTGTGATACCTACAGCCAGCCAATTGTTGCGCGCAAATCTTCTCACACGGTGAACAAGGCTTCTGTGCAAGCGCGT ATCAACAATATTACGGCGGCATTGGCAGAGCGGAGTGCGCCTTATATTGAGCGTGCTCATCACCAAGCAGAAGTATTGGG TCTGCCTTTCTTACCGACCACGACCATTGGCTCTTTCCCACAAACAGGAGAGATCCGTACTGAGCGTAGTGCGTATCGCC AAGGCAAGCTGAGCGAGCAAGAGTATGTGCAAGCACTGAAAGGTCATATTGCTGATGCGGTTAAACGCCAAGAAGCACTA GGGTTAGATGTGCTGGTGCATGGTGAAGCGGAACGTAACGACATGGTGGAATACTTTGCTGAAAACCTCGCTGGTTTCCA AACCACGCAGTTTGGTTGGGTGCAGAGTTACGGTTCACGCTGCGTAAAACCTGCGATTGTGGTGGCGGATATCGAACGTG AAAAACCGATCACGGTAGAGTGGTCAACTTACGCGCAATCTCTAACTTCTAAGCAGATGAAAGGAATGTTGACGGGGCCT GTGACCATTCTATGCTGGACCTTCCCACGTGAAGACATCAGCCGCCAAGAGATTGCTCAGCAATTAGCGTTGGCACTGCG TGATGAAGTGGCGGATTTGCAAGATGCAGGCATCAATATTATTCAAATTGACGAACCCGCGATTCGTGAAGGTTTACCAC TGAAAAAACGCGATCATCAAACCTATTTAGATTGGGCAGTGCAAGCGTTTAAGATTTCCGCGGGTAGCGCGCGTCCGGAA ACGCAAATTCATACCCATATGTGTTACAGCGAATTCAACGAGATCATCGAATCGGTTGCGGCACTGGATGCCGACGTGAT CACCATTGAAACATCGCGCTCCAACATGGAACTGCTCAAAGCGTTTGAAGAGTTTAACTACCCGAACGAAATTGGCCCAG GCGTGTACGACATTCACTCACCGAACATTCCTGCACAAACATGGATTGAAGATCTGCTGCGCAAAGCAGCAGAAAAAATT CCAGCACAGCGTTTGTGGGTCAACCCAGATTGCGGTCTAAAAACCCGTAACTGGCCAGAAGTGGAAGCGGCACTCACCAA CATGGTGAACGCAGCGAAAGCACTGCGCGCGGAATGGCAAGCTTGA
Upstream 100 bases:
>100_bases AGGCGTACAACGCGATAGTTTAGCCATCCAGACATCTTTCGATGTGCTGAGGCAGCTTAGAGCTTCTACGACCTATTAAG AACAGATAAAGGATTTTCAC
Downstream 100 bases:
>100_bases TATACGCTTCCTACTTGAGGTTTACCTACAACGCCAAGTAGTTTGAGTAAACCGTTAGAAACCGCAAAGGCCTCTTATGA GGCCTTTGTTCTTTTCGTTT
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 761; Mature: 760
Protein sequence:
>761_residues MTTTHILGYPCIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQHSWALQKEAGLDFVTAGDFAWYDHVLTTTLLLGHVP KRHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWFNTNYHYIVPEFSSHDTFNVSWPQLFEEVNEALQAGHDVKP VLLGPISYLYLGKEVEEGFDRLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLL LTTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRADLSAILARLQPVKTLLGERL WVASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVALLGRALEGDAAAILACDTYSQPIVARKSSHTVNKASVQAR INNITAALAERSAPYIERAHHQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEAL GLDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVEWSTYAQSLTSKQMKGMLTGP VTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINIIQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPE TQIHTHMCYSEFNEIIESVAALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQTWIEDLLRKAAEKI PAQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA
Sequences:
>Translated_761_residues MTTTHILGYPCIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQHSWALQKEAGLDFVTAGDFAWYDHVLTTTLLLGHVP KRHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWFNTNYHYIVPEFSSHDTFNVSWPQLFEEVNEALQAGHDVKP VLLGPISYLYLGKEVEEGFDRLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLL LTTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRADLSAILARLQPVKTLLGERL WVASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVALLGRALEGDAAAILACDTYSQPIVARKSSHTVNKASVQAR INNITAALAERSAPYIERAHHQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEAL GLDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVEWSTYAQSLTSKQMKGMLTGP VTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINIIQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPE TQIHTHMCYSEFNEIIESVAALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQTWIEDLLRKAAEKI PAQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA >Mature_760_residues TTTHILGYPCIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQHSWALQKEAGLDFVTAGDFAWYDHVLTTTLLLGHVPK RHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWFNTNYHYIVPEFSSHDTFNVSWPQLFEEVNEALQAGHDVKPV LLGPISYLYLGKEVEEGFDRLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLLL TTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRADLSAILARLQPVKTLLGERLW VASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVALLGRALEGDAAAILACDTYSQPIVARKSSHTVNKASVQARI NNITAALAERSAPYIERAHHQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEALG LDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVEWSTYAQSLTSKQMKGMLTGPV TILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINIIQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPET QIHTHMCYSEFNEIIESVAALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQTWIEDLLRKAAEKIP AQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=756, Percent_Identity=60.978835978836, Blast_Score=929, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=774, Percent_Identity=44.3152454780362, Blast_Score=660, Evalue=0.0,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 84987; Mature: 84856
Theoretical pI: Translated: 5.28; Mature: 5.28
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 0.9 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTTTHILGYPCIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQHSWALQKEAGLDFVTA CCCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEC GDFAWYDHVLTTTLLLGHVPKRHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWF CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH NTNYHYIVPEFSSHDTFNVSWPQLFEEVNEALQAGHDVKPVLLGPISYLYLGKEVEEGFD CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHCHHHHHHHH RLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLL HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHH LTTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRA HHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHH DLSAILARLQPVKTLLGERLWVASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVA HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH LLGRALEGDAAAILACDTYSQPIVARKSSHTVNKASVQARINNITAALAERSAPYIERAH HHHHHCCCCCEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH HQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEAL HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC GLDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVE CCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE WSTYAQSLTSKQMKGMLTGPVTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINI HHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEE IQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPETQIHTHMCYSEFNEIIESVA EEECCHHHHCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHH ALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQTWIEDLLRKAAEKI HCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHC PAQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA CHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure TTTHILGYPCIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQHSWALQKEAGLDFVTA CCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEC GDFAWYDHVLTTTLLLGHVPKRHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWF CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH NTNYHYIVPEFSSHDTFNVSWPQLFEEVNEALQAGHDVKPVLLGPISYLYLGKEVEEGFD CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHCHHHHHHHH RLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLL HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHH LTTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRA HHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHH DLSAILARLQPVKTLLGERLWVASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVA HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH LLGRALEGDAAAILACDTYSQPIVARKSSHTVNKASVQARINNITAALAERSAPYIERAH HHHHHCCCCCEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH HQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEAL HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC GLDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVE CCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE WSTYAQSLTSKQMKGMLTGPVTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINI HHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEE IQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPETQIHTHMCYSEFNEIIESVA EEECCHHHHCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHH ALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQTWIEDLLRKAAEKI HCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHC PAQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA CHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]