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The map label for this gene is focA [H]

Identifier: 147673760

GI number: 147673760

Start: 1382418

End: 1383329

Strand: Reverse

Name: focA [H]

Synonym: VC0395_A1299

Alternate gene names: 147673760

Gene position: 1383329-1382418 (Counterclockwise)

Preceding gene: 147675183

Following gene: 147674351

Centisome position: 45.74

GC content: 45.94

Gene sequence:

>912_bases
TTGAACTCAAGGGGAAATAGAGCGATTATCCGCCACCTCTTCTGTTGTGTGTCAAAATGTAACAAGCTCATGGAACATAA
CCAATTCGATTCTCTTCTCCCACCCCAGATGGCAGAACGTGCCGCCATTACGGGTGAAGGTAAAGCTAAAAAAGCGGCGT
ACAAATCTTTCCTGTTGGCGATTTCTGCTGGCATCCAAATCGGTATTGCCTTTGTTTTTTATACCGTAGTCACGACTGGT
GCTCACGATATGCCTTACGGGGTGACTAAGCTGCTCGGCGGCTTAGCTTTTAGCTTAGGTTTAATCCTAGTCGTCATTAC
TGGCGGTGAGCTGTTTACCAGCTCAGTGTTGATTTTGGTGGCGAAAGCGAGTGGTAAAATTTCGTGGAAAGAGCTGGTTC
GCAACTGGACGGTGGTCTACTTCGGTAACTTATGTGGTTCGATTATCTTAGTGTTTATCATGCTCGCCACCCGTCAGTTT
ATGGAAGATGGTGGTCAGCTTGGCTTAAATGCGATGGCGATTTCCCAGCACAAATTGCATCACACCTTCTTGCAAGCTTT
CGCGCTTGGATTGATGTGTAACATCCTAGTTTGTTTGGCGGTTTGGATGACCTTTAGTGCCCGCTCGTTAACTGATAAAG
TGATGGTCTTGATCCTGCCGGTGGCCATGTTTGTCTCTTCCGGTTTTGAGCACTGTATTGCCAATATGTTCCAAGTACCG
ATGGCGATTGGGATTAAGTATTTTGCGCCAGAGAGTTTTTGGGCAATGACAGGAGCGAATATCGCTCAGTACGCAGATCT
CAACTTTGTTAACTTCATCGTCAATAACCTCATTCCAGTGACCTTAGGCAATATTGTTGGTGGCGGTGTGTTTGTTGGCA
TGTGGTACTGGCTGATCTATCTAAAAGATTAA

Upstream 100 bases:

>100_bases
ACCGAACGCGAGAGTTGAGGTGAATAGACCGAGAAAACGGCACTGCAACTCCAAGTAGGACGGGTATCTAAACATTTTTC
GTGAACATGACAAATAGCCC

Downstream 100 bases:

>100_bases
TCCTGACAACCATCAGCGCTTCCCCAATACCCCACAATCCCTGAGTGTTACTCGGGGATTTTTTTACCTGTTTTTCCTTG
CACTCATGAGGTTATCATCG

Product: putative formate transporter 1

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNSRGNRAIIRHLFCCVSKCNKLMEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLAISAGIQIGIAFVFYTVVTTG
AHDMPYGVTKLLGGLAFSLGLILVVITGGELFTSSVLILVAKASGKISWKELVRNWTVVYFGNLCGSIILVFIMLATRQF
MEDGGQLGLNAMAISQHKLHHTFLQAFALGLMCNILVCLAVWMTFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVP
MAIGIKYFAPESFWAMTGANIAQYADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIYLKD

Sequences:

>Translated_303_residues
MNSRGNRAIIRHLFCCVSKCNKLMEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLAISAGIQIGIAFVFYTVVTTG
AHDMPYGVTKLLGGLAFSLGLILVVITGGELFTSSVLILVAKASGKISWKELVRNWTVVYFGNLCGSIILVFIMLATRQF
MEDGGQLGLNAMAISQHKLHHTFLQAFALGLMCNILVCLAVWMTFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVP
MAIGIKYFAPESFWAMTGANIAQYADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIYLKD
>Mature_303_residues
MNSRGNRAIIRHLFCCVSKCNKLMEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLAISAGIQIGIAFVFYTVVTTG
AHDMPYGVTKLLGGLAFSLGLILVVITGGELFTSSVLILVAKASGKISWKELVRNWTVVYFGNLCGSIILVFIMLATRQF
MEDGGQLGLNAMAISQHKLHHTFLQAFALGLMCNILVCLAVWMTFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVP
MAIGIKYFAPESFWAMTGANIAQYADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIYLKD

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=277, Percent_Identity=54.5126353790614, Blast_Score=318, Evalue=2e-88,
Organism=Escherichia coli, GI1788837, Length=283, Percent_Identity=41.3427561837456, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI87082249, Length=219, Percent_Identity=31.5068493150685, Blast_Score=74, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=230, Percent_Identity=27.3913043478261, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 33202; Mature: 33202

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRGNRAIIRHLFCCVSKCNKLMEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
ISAGIQIGIAFVFYTVVTTGAHDMPYGVTKLLGGLAFSLGLILVVITGGELFTSSVLILV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHEE
AKASGKISWKELVRNWTVVYFGNLCGSIILVFIMLATRQFMEDGGQLGLNAMAISQHKLH
EECCCCCCHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
HTFLQAFALGLMCNILVCLAVWMTFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MAIGIKYFAPESFWAMTGANIAQYADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIY
HHHCHHHCCCCHHHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKD
CCC
>Mature Secondary Structure
MNSRGNRAIIRHLFCCVSKCNKLMEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
ISAGIQIGIAFVFYTVVTTGAHDMPYGVTKLLGGLAFSLGLILVVITGGELFTSSVLILV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHEE
AKASGKISWKELVRNWTVVYFGNLCGSIILVFIMLATRQFMEDGGQLGLNAMAISQHKLH
EECCCCCCHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
HTFLQAFALGLMCNILVCLAVWMTFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MAIGIKYFAPESFWAMTGANIAQYADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIY
HHHCHHHCCCCHHHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]