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The map label for this gene is lepB [H]

Identifier: 147675126

GI number: 147675126

Start: 2197387

End: 2198283

Strand: Reverse

Name: lepB [H]

Synonym: VC0395_A2040

Alternate gene names: 147675126

Gene position: 2198283-2197387 (Counterclockwise)

Preceding gene: 147673257

Following gene: 147674970

Centisome position: 72.69

GC content: 49.28

Gene sequence:

>897_bases
ATGGCGAACACATTCTCACTGATTTTGGTGATCGTAACTCTGGTCACCGGTATCGTCTGGACACTGGAAAAGCTGGTGTG
GGCGAAAAAACGCCAACAGAAACAAGCTCATTTACAGGCGCAAACGCCCGATATGCCAGCCTCAGCGCTGGATAAAGTCG
TGGCTCAGCCGTGGTGGATTGAAAACAGTGTCTCGATTTTCCCTGTTATTGCTTTTGTGCTGGTACTGCGCTCGTTCATT
TATGAACCGTTCCAAATTCCATCCGGTTCGATGATGCCGACTCTGCTGGTCGGGGATTTTATTCTGGTTGAGAAATACGC
TTACGGCTTGAAAGATCCGGTATGGCGCACTCAGTTAGTGGAAACGGGTAAACCTGAGCGTGGTGATATTGTGGTGTTCA
AATACCCAGTGAACCCTGAGATCGACTACATCAAACGTGTGGTGGGGATGCCCGGAGATACCGTACGTTACAGCGCAGGT
AAAGAGCTGTGTATTCAGCACCAAGGCGAGAGCGAATGCCAAGCAGTTAAACTCTCTAACGTGCAAGAGAGCGAGTTTTA
CCAAAATGAGATCCCCCTGATCCAGCTGAACGAACAGCTAGGTAAGGTTGAGCACAATATTTTGGTTAACCCATTGAGCA
TTGATAACGTGGCGAATTATCGCCCACGCAGTGGCGTGAATGAATGGGTTGTACCACAAGGGCACTATTTTGTGATGGGT
GATAACCGTGACAACAGTGCTGACAGCCGTTTCTGGGGCTTTGTGCCAGAGCAGAATCTGGTCGGAAAAGCTGTGGCTAT
CTGGATCAGTTTCGAGTTTGAACGCGCTGAAGACAGCGTACTTCCACGCTGGATTCCTACCGGAGTACGATTCAATCGTG
TTGGTGGGATCCACTAA

Upstream 100 bases:

>100_bases
AAAGACTAATTTCTTTGCTGGCTTCAGACTTATTGGTTTAACACATGAGTGAAAGGGTTTCGGCTCTTTCACTTTCGTAT
TTTTTAGATAAGGGAAGTCA

Downstream 100 bases:

>100_bases
GCCGGCATGAGCTGGCAATAACGTTGAGATTATGACACCTCCAATGAATAAATTAACAAGTAAGCTCGGTTATACCTTTA
AGGAGACTGAGCTGCTTAAT

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MANTFSLILVIVTLVTGIVWTLEKLVWAKKRQQKQAHLQAQTPDMPASALDKVVAQPWWIENSVSIFPVIAFVLVLRSFI
YEPFQIPSGSMMPTLLVGDFILVEKYAYGLKDPVWRTQLVETGKPERGDIVVFKYPVNPEIDYIKRVVGMPGDTVRYSAG
KELCIQHQGESECQAVKLSNVQESEFYQNEIPLIQLNEQLGKVEHNILVNPLSIDNVANYRPRSGVNEWVVPQGHYFVMG
DNRDNSADSRFWGFVPEQNLVGKAVAIWISFEFERAEDSVLPRWIPTGVRFNRVGGIH

Sequences:

>Translated_298_residues
MANTFSLILVIVTLVTGIVWTLEKLVWAKKRQQKQAHLQAQTPDMPASALDKVVAQPWWIENSVSIFPVIAFVLVLRSFI
YEPFQIPSGSMMPTLLVGDFILVEKYAYGLKDPVWRTQLVETGKPERGDIVVFKYPVNPEIDYIKRVVGMPGDTVRYSAG
KELCIQHQGESECQAVKLSNVQESEFYQNEIPLIQLNEQLGKVEHNILVNPLSIDNVANYRPRSGVNEWVVPQGHYFVMG
DNRDNSADSRFWGFVPEQNLVGKAVAIWISFEFERAEDSVLPRWIPTGVRFNRVGGIH
>Mature_297_residues
ANTFSLILVIVTLVTGIVWTLEKLVWAKKRQQKQAHLQAQTPDMPASALDKVVAQPWWIENSVSIFPVIAFVLVLRSFIY
EPFQIPSGSMMPTLLVGDFILVEKYAYGLKDPVWRTQLVETGKPERGDIVVFKYPVNPEIDYIKRVVGMPGDTVRYSAGK
ELCIQHQGESECQAVKLSNVQESEFYQNEIPLIQLNEQLGKVEHNILVNPLSIDNVANYRPRSGVNEWVVPQGHYFVMGD
NRDNSADSRFWGFVPEQNLVGKAVAIWISFEFERAEDSVLPRWIPTGVRFNRVGGIH

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=332, Percent_Identity=53.0120481927711, Blast_Score=321, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR019766 [H]

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 33842; Mature: 33711

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTFSLILVIVTLVTGIVWTLEKLVWAKKRQQKQAHLQAQTPDMPASALDKVVAQPWWI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
ENSVSIFPVIAFVLVLRSFIYEPFQIPSGSMMPTLLVGDFILVEKYAYGLKDPVWRTQLV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ETGKPERGDIVVFKYPVNPEIDYIKRVVGMPGDTVRYSAGKELCIQHQGESECQAVKLSN
HCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEECCCCCEEEECCCCCHHHEEECCC
VQESEFYQNEIPLIQLNEQLGKVEHNILVNPLSIDNVANYRPRSGVNEWVVPQGHYFVMG
CCHHHHHHCCCCEEEECHHHCCHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
DNRDNSADSRFWGFVPEQNLVGKAVAIWISFEFERAEDSVLPRWIPTGVRFNRVGGIH
CCCCCCCCCCEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure 
ANTFSLILVIVTLVTGIVWTLEKLVWAKKRQQKQAHLQAQTPDMPASALDKVVAQPWWI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
ENSVSIFPVIAFVLVLRSFIYEPFQIPSGSMMPTLLVGDFILVEKYAYGLKDPVWRTQLV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ETGKPERGDIVVFKYPVNPEIDYIKRVVGMPGDTVRYSAGKELCIQHQGESECQAVKLSN
HCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEECCCCCEEEECCCCCHHHEEECCC
VQESEFYQNEIPLIQLNEQLGKVEHNILVNPLSIDNVANYRPRSGVNEWVVPQGHYFVMG
CCHHHHHHCCCCEEEECHHHCCHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
DNRDNSADSRFWGFVPEQNLVGKAVAIWISFEFERAEDSVLPRWIPTGVRFNRVGGIH
CCCCCCCCCCEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6311837; 9278503; 2202591; 1618816; 1546969; 8394311; 2551889; 9823901 [H]