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The map label for this gene is sucB [H]

Identifier: 147675118

GI number: 147675118

Start: 1798499

End: 1799713

Strand: Reverse

Name: sucB [H]

Synonym: VC0395_A1672

Alternate gene names: 147675118

Gene position: 1799713-1798499 (Counterclockwise)

Preceding gene: 147675042

Following gene: 147673132

Centisome position: 59.51

GC content: 49.22

Gene sequence:

>1215_bases
ATGACCATTGAAATTCTGGTTCCAGATTTGCCTGAATCAGTTGCGGATGCCACGGTTGCGACGTGGCATAAAAAGCCGGG
TGACATGGTTGCTCGCGATGAAGTGATCGTTGAAATTGAAACTGACAAAGTGGTGCTGGAAGTTCCAGCACCCGATGCCG
GTGTTCTGGAAGCTATTTTGGAACAAGAAGGTGCGACAGTGCTTTCTAAACAACTGCTGGCACGTTTGAAGCCAGGCGCT
GTAGCGGGTGAACCAACCCAAGATACTCCAGATGCTACTGAGCCATCACCAGATAAACGCCATAAAGCATCACTAACTGA
AGAAAGCAATGATGCGCTAAGCCCTGCGGTTCGTCGCCTGCTTGCTGAACATAACTTGGAAGCAAACCAAGTTAAAGGTT
CTGGTGTGGGTGGTCGTATTACCCGCGAAGACATCGAAGCTCACTTAGCCGCGAACAAAGCCAAACCAGCTGCGAAAGCT
GAAGCCCCTATCGCAGCGCTGGCTCCTGTCGTCGGCCGCAGTGAAAAGCGCGTGCCAATGACACGTCTGCGTAAGCGTAT
CGCTGAGCGTCTGTTGGAAGCGAAAAATAACACGGCTATGCTGACGACGTTTAATGAAGTGAACATGAAACCTATCATGG
ACATGCGTAAGCAATACCAAGATGTGTTCGAAAAACGTCATGGCATTCGTCTGGGCTTTATGTCGTTCTACGTGAAAGCT
GTCACTGAAGCGCTGAAGCGTTACCCAGAAGTGAACGCGTCTATCGATGGTGATGATCTGGTTTACCACAACTACTTTGA
TGTGAGCATTGCCGTATCAACCCCACGTGGTCTGGTTACTCCAGTACTGAAAAACTGCGATACCTTGAGTTTGGCGCAAA
TTGAAAAAGGCATCAAAGAGCTAGCAGAAAAAGGTCGTGACGGTAAATTGACCGTAGACGAACTGACTGGCGGTAACTTT
ACCATCACCAACGGTGGTGTGTTTGGTTCACTGATGTCAACGCCTATCATTAACCCGCCACAAGCGGCGATTCTGGGTAT
GCATAAGATCCAAGACCGCGCCATGGTGGTGGATGGCAAGATTGAAATCCTGCCAATGATGTATCTAGCGCTGTCTTATG
ACCACCGTTCAATCGATGGTCGTGAGTCGGTTGGTTTCCTAGTGACCGTTAAAGAGTTGCTAGAAGATCCAGCCCGTCTG
CTGCTCGATGTTTAA

Upstream 100 bases:

>100_bases
GCGGTCGGCTATATGTCGGTGCACATGAAACAACAAAAAGCGTTGATTGAAGACGCTCTGACCCTAGCGTAAGAACGAGA
AGTATAAGGAAGATACCATT

Downstream 100 bases:

>100_bases
ACTGTCTTTTGCTCAGATTTCTGAGTAATGGAGAAAAGGGTCGGACATGCTCAACGTCCGACCTGGCTTTGGCCAAAGAT
TGGCTACTCGCTCAAGAGTG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTIEILVPDLPESVADATVATWHKKPGDMVARDEVIVEIETDKVVLEVPAPDAGVLEAILEQEGATVLSKQLLARLKPGA
VAGEPTQDTPDATEPSPDKRHKASLTEESNDALSPAVRRLLAEHNLEANQVKGSGVGGRITREDIEAHLAANKAKPAAKA
EAPIAALAPVVGRSEKRVPMTRLRKRIAERLLEAKNNTAMLTTFNEVNMKPIMDMRKQYQDVFEKRHGIRLGFMSFYVKA
VTEALKRYPEVNASIDGDDLVYHNYFDVSIAVSTPRGLVTPVLKNCDTLSLAQIEKGIKELAEKGRDGKLTVDELTGGNF
TITNGGVFGSLMSTPIINPPQAAILGMHKIQDRAMVVDGKIEILPMMYLALSYDHRSIDGRESVGFLVTVKELLEDPARL
LLDV

Sequences:

>Translated_404_residues
MTIEILVPDLPESVADATVATWHKKPGDMVARDEVIVEIETDKVVLEVPAPDAGVLEAILEQEGATVLSKQLLARLKPGA
VAGEPTQDTPDATEPSPDKRHKASLTEESNDALSPAVRRLLAEHNLEANQVKGSGVGGRITREDIEAHLAANKAKPAAKA
EAPIAALAPVVGRSEKRVPMTRLRKRIAERLLEAKNNTAMLTTFNEVNMKPIMDMRKQYQDVFEKRHGIRLGFMSFYVKA
VTEALKRYPEVNASIDGDDLVYHNYFDVSIAVSTPRGLVTPVLKNCDTLSLAQIEKGIKELAEKGRDGKLTVDELTGGNF
TITNGGVFGSLMSTPIINPPQAAILGMHKIQDRAMVVDGKIEILPMMYLALSYDHRSIDGRESVGFLVTVKELLEDPARL
LLDV
>Mature_403_residues
TIEILVPDLPESVADATVATWHKKPGDMVARDEVIVEIETDKVVLEVPAPDAGVLEAILEQEGATVLSKQLLARLKPGAV
AGEPTQDTPDATEPSPDKRHKASLTEESNDALSPAVRRLLAEHNLEANQVKGSGVGGRITREDIEAHLAANKAKPAAKAE
APIAALAPVVGRSEKRVPMTRLRKRIAERLLEAKNNTAMLTTFNEVNMKPIMDMRKQYQDVFEKRHGIRLGFMSFYVKAV
TEALKRYPEVNASIDGDDLVYHNYFDVSIAVSTPRGLVTPVLKNCDTLSLAQIEKGIKELAEKGRDGKLTVDELTGGNFT
ITNGGVFGSLMSTPIINPPQAAILGMHKIQDRAMVVDGKIEILPMMYLALSYDHRSIDGRESVGFLVTVKELLEDPARLL
LDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=55.982905982906, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI31711992, Length=438, Percent_Identity=30.5936073059361, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI203098753, Length=449, Percent_Identity=27.8396436525612, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI203098816, Length=449, Percent_Identity=27.8396436525612, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=28.4064665127021, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=35.2201257861635, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=76.4267990074442, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1786305, Length=431, Percent_Identity=31.322505800464, Blast_Score=161, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=403, Percent_Identity=42.9280397022332, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=30.2059496567506, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=425, Percent_Identity=24.4705882352941, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=31.875, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320352, Length=408, Percent_Identity=42.8921568627451, Blast_Score=320, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=28.8546255506608, Blast_Score=163, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=57.5221238938053, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI18859875, Length=432, Percent_Identity=29.3981481481481, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24582497, Length=294, Percent_Identity=34.6938775510204, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI20129315, Length=294, Percent_Identity=34.6938775510204, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44091; Mature: 43960

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEILVPDLPESVADATVATWHKKPGDMVARDEVIVEIETDKVVLEVPAPDAGVLEAIL
CEEEEECCCCCHHHHHHHHHHHCCCCCCCEECCCEEEEEECCEEEEEECCCCHHHHHHHH
EQEGATVLSKQLLARLKPGAVAGEPTQDTPDATEPSPDKRHKASLTEESNDALSPAVRRL
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
LAEHNLEANQVKGSGVGGRITREDIEAHLAANKAKPAAKAEAPIAALAPVVGRSEKRVPM
HHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCCCH
TRLRKRIAERLLEAKNNTAMLTTFNEVNMKPIMDMRKQYQDVFEKRHGIRLGFMSFYVKA
HHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHH
VTEALKRYPEVNASIDGDDLVYHNYFDVSIAVSTPRGLVTPVLKNCDTLSLAQIEKGIKE
HHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHH
LAEKGRDGKLTVDELTGGNFTITNGGVFGSLMSTPIINPPQAAILGMHKIQDRAMVVDGK
HHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCC
IEILPMMYLALSYDHRSIDGRESVGFLVTVKELLEDPARLLLDV
CHHHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCHHHHHCCC
>Mature Secondary Structure 
TIEILVPDLPESVADATVATWHKKPGDMVARDEVIVEIETDKVVLEVPAPDAGVLEAIL
EEEEECCCCCHHHHHHHHHHHCCCCCCCEECCCEEEEEECCEEEEEECCCCHHHHHHHH
EQEGATVLSKQLLARLKPGAVAGEPTQDTPDATEPSPDKRHKASLTEESNDALSPAVRRL
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
LAEHNLEANQVKGSGVGGRITREDIEAHLAANKAKPAAKAEAPIAALAPVVGRSEKRVPM
HHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCCCH
TRLRKRIAERLLEAKNNTAMLTTFNEVNMKPIMDMRKQYQDVFEKRHGIRLGFMSFYVKA
HHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHH
VTEALKRYPEVNASIDGDDLVYHNYFDVSIAVSTPRGLVTPVLKNCDTLSLAQIEKGIKE
HHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHH
LAEKGRDGKLTVDELTGGNFTITNGGVFGSLMSTPIINPPQAAILGMHKIQDRAMVVDGK
HHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCC
IEILPMMYLALSYDHRSIDGRESVGFLVTVKELLEDPARLLLDV
CHHHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]