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The map label for this gene is gltX [H]

Identifier: 147675108

GI number: 147675108

Start: 1919897

End: 1921426

Strand: Reverse

Name: gltX [H]

Synonym: VC0395_A1806

Alternate gene names: 147675108

Gene position: 1921426-1919897 (Counterclockwise)

Preceding gene: 147673355

Following gene: 147673624

Centisome position: 63.54

GC content: 48.5

Gene sequence:

>1530_bases
TTGCCGAAAATCGTGCGTAGCATCGCGTTAACTCGGGTGTTAGAATACCGCCCAAAATTTCGCATGCTGAGCTCTCAGCA
AAAGAATCTCACAAAGGTAATTCAAATGACGGTTAAAACTCGTTTTGCGCCTAGTCCAACTGGGTATCTGCACGTTGGTG
GTGCGCGTACAGCACTCTATTCTTGGTTATATGCTAAAAGCCAAGGTGGTGAATTTGTTCTGCGTATTGAAGACACCGAC
CTAGAACGCTCTACTCAGGCGGCGGTGGATGCCATTATTGAAGGCATGACGTGGTTAGGTTTGGAATGGGATGAAGGCCC
ATACTACCAAACCAAACGTTTTGATCGTTACAACCAAGTGATTGATCAGCTATTGGCAGAAGGCAAAGCCTACAAGTGCT
ATGCGCCGAAAGAGCTGCTGGATGAAATCCGTGCTGAGCAAGAAGCCAACAAAGAGATGCCTCGCTACGATGCGAATCAT
CCAAAAATCAAAGCGGTCAATGATGCGGCAAAAGAAGGTGAGCCTTGCTGTATCCGTTTCCGTAACCCGAAAGAGGGGAG
CGTCGTTTTTGATGACCAAATCCGTGGCCGCATCGAAATCCGTAACGACCAACTGGATGATCTGATCATCCGTCGTACTG
ATGGCACTCCAACCTATAACTTCTGTGTAGTCGTGGATGATGTCGATATGGGTATCAGCCATGTGATTCGTGGTGAAGAC
CACATCAACAACACGCCACGCCAGATCAACATTTACAAAGCCATGGGCGCGACCATCCCAACCTTTGCGCACTGTGCAAT
GATCTTGGGTGATGATGGTGCCAAGCTATCTAAGCGCCATGGCGCGGTTTCTGTAATGCAATACCGTGATGATGGTTACC
TCCCAGAAGCGCTGCTGAACTACTTAGTGCGTTTAGGTTGGGGACATGGCGACCAAGAGATCTTCTCACGCGATGAAATG
ATCAACTTATTCAGCCTTAACGCAATTTCAAAATCAGCGTCAGCGTTTAACACTGACAAACTGCTGTGGTTGAACAACCA
CTACATCAAGACTTCAGAGCCTGAGTATGTGGCGAAACACCTTGAGTGGCATTTCGAGAACCAAGGCATTAACAAAGCGA
CCGGCCCTGCTTTAGCGGAAGTGGTGAAGTTGGTGGGTGAGCGTTGCAATACCTTGGTTGAGCTTGCGCAGCAATCACGT
TACTTCTACGAAGATTTTGCTGAGTTTGATGCTGATGCCGCGAAGAAACACCTGCGTGGTGTGGCAAAAGAGCCACTGAT
GCTAGCTCTGAGCAAGATTGAAGCGCTGACAGAATGGAATACCGAAGCACTGCATCACGTGATCGCACAAGTGTGTGAAG
AGCTTGAGATTGGCATGGGTAAAATCGGTATGCCACTGCGTGTTGCTGTGACTGGCGGTGGCCAATCGCCTTCTGTGGAT
GCGGTGATGAACTTGATTGGTCAAGAACGTGTAATTGCACGTATCAAAATGGCACTAGAGTACATCGAAACTCGCGAAGC
GAATGCATAG

Upstream 100 bases:

>100_bases
TTCATGATCACTCCTCCTTGCGGAATGTCCACAAGGATAAACCTTAACCTTAAGGTAAGGTCAACCCTTACTTCTCGAAC
TCATTTTACTGAGAGTCAAT

Downstream 100 bases:

>100_bases
TTGTTTGATTGCATAAAAAGCCAAAGCCGCAGAACAAACTGCGGCTTTTTTATTATCTTTTGAAGTCTTGTTCTAATTCT
ACCGTTTATAGTTAAGGGTA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPKIVRSIALTRVLEYRPKFRMLSSQQKNLTKVIQMTVKTRFAPSPTGYLHVGGARTALYSWLYAKSQGGEFVLRIEDTD
LERSTQAAVDAIIEGMTWLGLEWDEGPYYQTKRFDRYNQVIDQLLAEGKAYKCYAPKELLDEIRAEQEANKEMPRYDANH
PKIKAVNDAAKEGEPCCIRFRNPKEGSVVFDDQIRGRIEIRNDQLDDLIIRRTDGTPTYNFCVVVDDVDMGISHVIRGED
HINNTPRQINIYKAMGATIPTFAHCAMILGDDGAKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWGHGDQEIFSRDEM
INLFSLNAISKSASAFNTDKLLWLNNHYIKTSEPEYVAKHLEWHFENQGINKATGPALAEVVKLVGERCNTLVELAQQSR
YFYEDFAEFDADAAKKHLRGVAKEPLMLALSKIEALTEWNTEALHHVIAQVCEELEIGMGKIGMPLRVAVTGGGQSPSVD
AVMNLIGQERVIARIKMALEYIETREANA

Sequences:

>Translated_509_residues
MPKIVRSIALTRVLEYRPKFRMLSSQQKNLTKVIQMTVKTRFAPSPTGYLHVGGARTALYSWLYAKSQGGEFVLRIEDTD
LERSTQAAVDAIIEGMTWLGLEWDEGPYYQTKRFDRYNQVIDQLLAEGKAYKCYAPKELLDEIRAEQEANKEMPRYDANH
PKIKAVNDAAKEGEPCCIRFRNPKEGSVVFDDQIRGRIEIRNDQLDDLIIRRTDGTPTYNFCVVVDDVDMGISHVIRGED
HINNTPRQINIYKAMGATIPTFAHCAMILGDDGAKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWGHGDQEIFSRDEM
INLFSLNAISKSASAFNTDKLLWLNNHYIKTSEPEYVAKHLEWHFENQGINKATGPALAEVVKLVGERCNTLVELAQQSR
YFYEDFAEFDADAAKKHLRGVAKEPLMLALSKIEALTEWNTEALHHVIAQVCEELEIGMGKIGMPLRVAVTGGGQSPSVD
AVMNLIGQERVIARIKMALEYIETREANA
>Mature_508_residues
PKIVRSIALTRVLEYRPKFRMLSSQQKNLTKVIQMTVKTRFAPSPTGYLHVGGARTALYSWLYAKSQGGEFVLRIEDTDL
ERSTQAAVDAIIEGMTWLGLEWDEGPYYQTKRFDRYNQVIDQLLAEGKAYKCYAPKELLDEIRAEQEANKEMPRYDANHP
KIKAVNDAAKEGEPCCIRFRNPKEGSVVFDDQIRGRIEIRNDQLDDLIIRRTDGTPTYNFCVVVDDVDMGISHVIRGEDH
INNTPRQINIYKAMGATIPTFAHCAMILGDDGAKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWGHGDQEIFSRDEMI
NLFSLNAISKSASAFNTDKLLWLNNHYIKTSEPEYVAKHLEWHFENQGINKATGPALAEVVKLVGERCNTLVELAQQSRY
FYEDFAEFDADAAKKHLRGVAKEPLMLALSKIEALTEWNTEALHHVIAQVCEELEIGMGKIGMPLRVAVTGGGQSPSVDA
VMNLIGQERVIARIKMALEYIETREANA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=505, Percent_Identity=31.4851485148515, Blast_Score=225, Evalue=9e-59,
Organism=Homo sapiens, GI62241042, Length=361, Percent_Identity=23.2686980609418, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=69.8513800424628, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=32.7935222672065, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1786895, Length=133, Percent_Identity=30.0751879699248, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=492, Percent_Identity=30.6910569105691, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=494, Percent_Identity=30.3643724696356, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321192, Length=216, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324742, Length=218, Percent_Identity=26.605504587156, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=32.9959514170041, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 57535; Mature: 57404

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIVRSIALTRVLEYRPKFRMLSSQQKNLTKVIQMTVKTRFAPSPTGYLHVGGARTALY
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
SWLYAKSQGGEFVLRIEDTDLERSTQAAVDAIIEGMTWLGLEWDEGPYYQTKRFDRYNQV
HHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHCCEECCCCCCCHHHHHHHHHHH
IDQLLAEGKAYKCYAPKELLDEIRAEQEANKEMPRYDANHPKIKAVNDAAKEGEPCCIRF
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHCCCCEEEEE
RNPKEGSVVFDDQIRGRIEIRNDQLDDLIIRRTDGTPTYNFCVVVDDVDMGISHVIRGED
CCCCCCCEEECCCCCCEEEECCCCCCCEEEEECCCCCCEEEEEEEECCCCCHHHHHCCCH
HINNTPRQINIYKAMGATIPTFAHCAMILGDDGAKLSKRHGAVSVMQYRDDGYLPEALLN
HCCCCCCEEEEEHHHCCCCHHHHHHHHEECCCCCHHHHHCCCEEEEEECCCCCCHHHHHH
YLVRLGWGHGDQEIFSRDEMINLFSLNAISKSASAFNTDKLLWLNNHYIKTSEPEYVAKH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEECCCCHHHHHHH
LEWHFENQGINKATGPALAEVVKLVGERCNTLVELAQQSRYFYEDFAEFDADAAKKHLRG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VAKEPLMLALSKIEALTEWNTEALHHVIAQVCEELEIGMGKIGMPLRVAVTGGGQSPSVD
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHH
AVMNLIGQERVIARIKMALEYIETREANA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PKIVRSIALTRVLEYRPKFRMLSSQQKNLTKVIQMTVKTRFAPSPTGYLHVGGARTALY
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
SWLYAKSQGGEFVLRIEDTDLERSTQAAVDAIIEGMTWLGLEWDEGPYYQTKRFDRYNQV
HHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHCCEECCCCCCCHHHHHHHHHHH
IDQLLAEGKAYKCYAPKELLDEIRAEQEANKEMPRYDANHPKIKAVNDAAKEGEPCCIRF
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHCCCCEEEEE
RNPKEGSVVFDDQIRGRIEIRNDQLDDLIIRRTDGTPTYNFCVVVDDVDMGISHVIRGED
CCCCCCCEEECCCCCCEEEECCCCCCCEEEEECCCCCCEEEEEEEECCCCCHHHHHCCCH
HINNTPRQINIYKAMGATIPTFAHCAMILGDDGAKLSKRHGAVSVMQYRDDGYLPEALLN
HCCCCCCEEEEEHHHCCCCHHHHHHHHEECCCCCHHHHHCCCEEEEEECCCCCCHHHHHH
YLVRLGWGHGDQEIFSRDEMINLFSLNAISKSASAFNTDKLLWLNNHYIKTSEPEYVAKH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEECCCCHHHHHHH
LEWHFENQGINKATGPALAEVVKLVGERCNTLVELAQQSRYFYEDFAEFDADAAKKHLRG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VAKEPLMLALSKIEALTEWNTEALHHVIAQVCEELEIGMGKIGMPLRVAVTGGGQSPSVD
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHH
AVMNLIGQERVIARIKMALEYIETREANA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA