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The map label for this gene is eno [H]

Identifier: 147675028

GI number: 147675028

Start: 2179798

End: 2181099

Strand: Reverse

Name: eno [H]

Synonym: VC0395_A2025

Alternate gene names: 147675028

Gene position: 2181099-2179798 (Counterclockwise)

Preceding gene: 147675626

Following gene: 147674552

Centisome position: 72.12

GC content: 47.93

Gene sequence:

>1302_bases
ATGTCTAAGATCGTTAAAGTTCTAGGTCGTGAAATCATCGACTCACGTGGTAACCCAACGGTAGAAGCAGAAGTTCATCT
AGAAGGTGGTTTCGTTGGTATGGCAGCAGCTCCATCTGGTGCTTCTACTGGTTCTCGTGAAGCGCTTGAACTGCGTGACG
GTGACAAGTCTCGTTTCCTAGGTAAAGGCGTTCTGAAAGCGCTGGCTGCGGTTAATGGTCCAATCGCAGACGCTCTGGTT
GGCAAAGATGCAAAAGATCAAGCAACTATTGACCAAATCATGATCGACCTAGACGGTACTGAGAACAAGTCAAACTTCGG
TGCAAACGCGATTCTAGCGGTTTCTCTAGCAAACGCGAAAGCAGCAGCGGCAGCAAAAGGCATGCCTCTATACGAGCACA
TCGCTGAGCTGAACGGCACTCCTGGCGTATTCTCAATGCCTCTACCAATGATGAACATCATCAACGGTGGTGAGCACGCT
GACAACAACGTAGACATCCAAGAGTTCATGATCCAACCTGTTGGCGCAAAAACTCTGAAAGAAGCAGTACGTATGGGCGC
AGAAGTGTTCCATAACCTAGCTAAAGTACTGAAGTCTAAAGGCTACAACACTGCAGTGGGTGACGAAGGTGGTTTCGCTC
CTAACCTGAAATCTAACGCAGAAGCGCTAGAAGTTATCGCTGAAGCGGTTGCGGCTGCAGGTTACAAACTGGGTACTGAC
ATCACATTAGCGATGGACTGTGCGGCTTCTGAGTTCTACGACGCAGAGAAGAAAGAATACAACCTGAAAGGCGAAGGTCG
TATCTTCACTTCTAACGGTTTCTCTGATTTCCTAGAAGAGCTGACTGAGAAGTTCCCAATCGTTTCTATCGAAGACGGTC
TGGACGAGTCTGACTGGGAAGGTTTCGCATACCAAACTGAGAAACTGGGTAAGAAAATCCAAATCGTTGGTGACGATCTG
TTCGTAACTAACACTAAGATCCTGAAGCGTGGTATCGACAACGGTATCGCTAACTCTATCCTGATCAAGTTCAACCAAAT
CGGTTCTTTGACTGAAACTCTGGCTGCGATCAAGATGGCAAAAGATGCTGGCTACACTGCAGTTATCTCTCACCGTTCAG
GCGAAACTGAAGACGCAACTATCGCTGACCTAGCGGTAGGTACTGCTGCAGGTCAAATCAAGACGGGTTCTATGAGCCGT
TCTGACCGTGTTGCTAAGTACAACCAACTGATCCGTATCGAAGAAGCTCTGGGTTCACGTGCTCCTTTCAACGGTCTGAA
AGAAGTGAAAGGCCAAGCTTAA

Upstream 100 bases:

>100_bases
CCAACGCGGTGAATTGAAGTAAAAGGATACGGGTAGCCACTTTAGCAGTGTGGCTACCCTTTTAGTTTTGACATTTAAAT
TCAATTGAGAGGAAACATTA

Downstream 100 bases:

>100_bases
TTTTAAGCGAGCGCCTTCGCGACTGTTGACGATAACAGTCTCGCGATAAAAAACGCCCTGCATTGCAGGGCGTTTTGCTT
TCTACGGTTGCAGGCAACGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVLKALAAVNGPIADALV
GKDAKDQATIDQIMIDLDGTENKSNFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGVFSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEAVRMGAEVFHNLAKVLKSKGYNTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYKLGTD
ITLAMDCAASEFYDAEKKEYNLKGEGRIFTSNGFSDFLEELTEKFPIVSIEDGLDESDWEGFAYQTEKLGKKIQIVGDDL
FVTNTKILKRGIDNGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALGSRAPFNGLKEVKGQA

Sequences:

>Translated_433_residues
MSKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVLKALAAVNGPIADALV
GKDAKDQATIDQIMIDLDGTENKSNFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGVFSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEAVRMGAEVFHNLAKVLKSKGYNTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYKLGTD
ITLAMDCAASEFYDAEKKEYNLKGEGRIFTSNGFSDFLEELTEKFPIVSIEDGLDESDWEGFAYQTEKLGKKIQIVGDDL
FVTNTKILKRGIDNGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALGSRAPFNGLKEVKGQA
>Mature_432_residues
SKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVLKALAAVNGPIADALVG
KDAKDQATIDQIMIDLDGTENKSNFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGVFSMPLPMMNIINGGEHAD
NNVDIQEFMIQPVGAKTLKEAVRMGAEVFHNLAKVLKSKGYNTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYKLGTDI
TLAMDCAASEFYDAEKKEYNLKGEGRIFTSNGFSDFLEELTEKFPIVSIEDGLDESDWEGFAYQTEKLGKKIQIVGDDLF
VTNTKILKRGIDNGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRS
DRVAKYNQLIRIEEALGSRAPFNGLKEVKGQA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=53.8283062645012, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=53.8283062645012, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.3255813953488, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=52.4361948955916, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=48.951048951049, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=28.8690476190476, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=28.8690476190476, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=28.8690476190476, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1789141, Length=434, Percent_Identity=87.5576036866359, Blast_Score=742, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=51.3953488372093, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=51.3953488372093, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=44.4444444444444, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=49.3055555555556, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=46.5437788018433, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=46.5437788018433, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=46.5437788018433, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=47.6851851851852, Blast_Score=355, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=51.3953488372093, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=51.3953488372093, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=51.3953488372093, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=51.3953488372093, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=51.3953488372093, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=51.3953488372093, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45807; Mature: 45676

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFL
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCHHHHH
GKGVLKALAAVNGPIADALVGKDAKDQATIDQIMIDLDGTENKSNFGANAILAVSLANAK
HHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHEEEECCCCCCCCCCCCCEEEEEEECCCH
AAAAAKGMPLYEHIAELNGTPGVFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHCCCHHHHHHHHCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EAVRMGAEVFHNLAKVLKSKGYNTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYKLGTD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
ITLAMDCAASEFYDAEKKEYNLKGEGRIFTSNGFSDFLEELTEKFPIVSIEDGLDESDWE
EEEEEHHHHHHHHCCHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCC
GFAYQTEKLGKKIQIVGDDLFVTNTKILKRGIDNGIANSILIKFNQIGSLTETLAAIKMA
CCCHHHHHHCCEEEEEECCEEEECHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGSR
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
APFNGLKEVKGQA
CCCCCHHHHCCCC
>Mature Secondary Structure 
SKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFL
HHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCHHHHH
GKGVLKALAAVNGPIADALVGKDAKDQATIDQIMIDLDGTENKSNFGANAILAVSLANAK
HHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHEEEECCCCCCCCCCCCCEEEEEEECCCH
AAAAAKGMPLYEHIAELNGTPGVFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHCCCHHHHHHHHCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EAVRMGAEVFHNLAKVLKSKGYNTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYKLGTD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
ITLAMDCAASEFYDAEKKEYNLKGEGRIFTSNGFSDFLEELTEKFPIVSIEDGLDESDWE
EEEEEHHHHHHHHCCHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCC
GFAYQTEKLGKKIQIVGDDLFVTNTKILKRGIDNGIANSILIKFNQIGSLTETLAAIKMA
CCCHHHHHHCCEEEEEECCEEEECHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGSR
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
APFNGLKEVKGQA
CCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA