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The map label for this gene is pyrG [H]

Identifier: 147675626

GI number: 147675626

Start: 2181178

End: 2182815

Strand: Reverse

Name: pyrG [H]

Synonym: VC0395_A2026

Alternate gene names: 147675626

Gene position: 2182815-2181178 (Counterclockwise)

Preceding gene: 147673315

Following gene: 147675028

Centisome position: 72.18

GC content: 49.45

Gene sequence:

>1638_bases
ATGACGACAAATTATATTTTTGTTACTGGCGGGGTTGTATCCTCTCTAGGTAAAGGTATTGCAGCAGCATCCCTTGCAGC
GATTCTTGAAGCTCGTGGTCTTAAAGTAACCATGATGAAGCTTGACCCTTACATCAACGTTGACCCGGGCACCATGAGTC
CAACTCAACACGGTGAAGTGTTCGTTACGGAAGATGGCGCAGAAACCGACCTAGACCTTGGTCACTACGAGCGTTTCATT
CGTACCAAGATGACCAAGCGCAACAACTTTACCGCAGGTCGTGTTTACGCTGATGTTCTGCGCAAAGAGCGCCGTGGCGA
CTACTTAGGTGCCACGATTCAGGTTATTCCTCATATCACTAACGCGATCAAAGATCGTGTGATTGCGGGCTCAGAAGGCC
ACGACATTGCTATCGTTGAAGTTGGCGGTACTGTGGGTGATATCGAATCACTGCCATTTATGGAAGCGATCCGTCAGCTT
GCCATCGAAGTGGGTCGTGAGCACGCTATGTTCATGCATCTGACGCTGGTTCCTTACCTAGCGGCAGCGGGCGAAGTAAA
AACGAAGCCAACCCAACACTCAGTAAAAGAGCTGCTTTCTATCGGCATTCAGCCAGACATTCTGGTTTGCCGTAGCGATC
GTATGATCCCAGCCAACGAGCGTAAGAAAATTGCTCTGTTCTGTAACGTGCCTGAAAAAGCCGTTATCTCAATGAAAGAC
GTGGATTCTATCTACAAGATCCCACAACTGATCCGCTCTCAAGGGTTGGATGATTTGGTGTGTGCACGTTTTGGCATTAA
CGCGCCAGAAGCGGATCTCTCTGAATGGGAACAGGTGATTTACGAAGAAGCGAACCCAACCGGTGAAGTGACTATCGGTA
TGGTTGGTAAGTACACTGAACTGCCAGATGCTTACAAATCGGTCAACGAAGCCCTGAAACACGCCGGTCTGAAGAACCGT
CTGAGCGTGACCATCAAATACGTAGATTCACAAGACGTTGAAACCAAAGGCACCGATGTGCTCAATGGTTTGGATGCGAT
TCTGGTACCGGGTGGTTTTGGCGATCGTGGTATCGAAGGCAAAATCCGTGCAGCGCAATATGCACGTGAAAACAAAATTC
CTTACCTTGGCATCTGCTTGGGTATGCAAGTGGCACTCATCGAATACGCGCGCAACGTAGCGGGTATGGAAGGTGCACAC
TCAACCGAATTCAATAAAAACACCAAATATCCAGTGGTTGGTTTGATCACGGAATGGGTAGATGGCGAAGGCAATGTCGA
AGAGCGTAGCGAAAAATCGGATCTGGGCGGCACAATGCGTCTAGGTTCACAGCTATGCCACCTTGAGAAAGGCACTAAAG
CGTACGAGTTGTACGGTAGCGCAACCATTCATGAGCGTCACCGCCACCGTTACGAAGTCAACAACCTGCTGCGTCCTCAG
ATTGAAAAAGCAGGTTTGAAGGTTTCTGGTCTGTCAGCAGATAAGAAGCTGGTTGAAGTGATTGAGAACCCTGCTCACCC
ATGGTTTGTGGCAGCGCAGTTCCACCCAGAGTTCACCTCAACCCCACGCGATGGTCACCCTCTGTTTGCTGGCTTTGTAA
AAGCAGCGGGTCAGTTCCAACGCGGTGAATTGAAGTAA

Upstream 100 bases:

>100_bases
TGTGATAGATTTCACGTTGTGGCAATGGGGGCCTTCTGGTATAGTTTCGTCCCGTCCAGAAGAAATCCATTTCCCTCATT
CAACCAATTTCAGGTTAAAC

Downstream 100 bases:

>100_bases
AAGGATACGGGTAGCCACTTTAGCAGTGTGGCTACCCTTTTAGTTTTGACATTTAAATTCAATTGAGAGGAAACATTAAT
GTCTAAGATCGTTAAAGTTC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHITNAIKDRVIAGSEGHDIAIVEVGGTVGDIESLPFMEAIRQL
AIEVGREHAMFMHLTLVPYLAAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRMIPANERKKIALFCNVPEKAVISMKD
VDSIYKIPQLIRSQGLDDLVCARFGINAPEADLSEWEQVIYEEANPTGEVTIGMVGKYTELPDAYKSVNEALKHAGLKNR
LSVTIKYVDSQDVETKGTDVLNGLDAILVPGGFGDRGIEGKIRAAQYARENKIPYLGICLGMQVALIEYARNVAGMEGAH
STEFNKNTKYPVVGLITEWVDGEGNVEERSEKSDLGGTMRLGSQLCHLEKGTKAYELYGSATIHERHRHRYEVNNLLRPQ
IEKAGLKVSGLSADKKLVEVIENPAHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGQFQRGELK

Sequences:

>Translated_545_residues
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHITNAIKDRVIAGSEGHDIAIVEVGGTVGDIESLPFMEAIRQL
AIEVGREHAMFMHLTLVPYLAAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRMIPANERKKIALFCNVPEKAVISMKD
VDSIYKIPQLIRSQGLDDLVCARFGINAPEADLSEWEQVIYEEANPTGEVTIGMVGKYTELPDAYKSVNEALKHAGLKNR
LSVTIKYVDSQDVETKGTDVLNGLDAILVPGGFGDRGIEGKIRAAQYARENKIPYLGICLGMQVALIEYARNVAGMEGAH
STEFNKNTKYPVVGLITEWVDGEGNVEERSEKSDLGGTMRLGSQLCHLEKGTKAYELYGSATIHERHRHRYEVNNLLRPQ
IEKAGLKVSGLSADKKLVEVIENPAHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGQFQRGELK
>Mature_544_residues
TTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIR
TKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHITNAIKDRVIAGSEGHDIAIVEVGGTVGDIESLPFMEAIRQLA
IEVGREHAMFMHLTLVPYLAAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRMIPANERKKIALFCNVPEKAVISMKDV
DSIYKIPQLIRSQGLDDLVCARFGINAPEADLSEWEQVIYEEANPTGEVTIGMVGKYTELPDAYKSVNEALKHAGLKNRL
SVTIKYVDSQDVETKGTDVLNGLDAILVPGGFGDRGIEGKIRAAQYARENKIPYLGICLGMQVALIEYARNVAGMEGAHS
TEFNKNTKYPVVGLITEWVDGEGNVEERSEKSDLGGTMRLGSQLCHLEKGTKAYELYGSATIHERHRHRYEVNNLLRPQI
EKAGLKVSGLSADKKLVEVIENPAHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGQFQRGELK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=556, Percent_Identity=43.8848920863309, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=556, Percent_Identity=43.705035971223, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=556, Percent_Identity=43.705035971223, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=556, Percent_Identity=43.705035971223, Blast_Score=455, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=79.4495412844037, Blast_Score=897, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=38.9162561576355, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319432, Length=574, Percent_Identity=41.98606271777, Blast_Score=445, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322563, Length=565, Percent_Identity=41.2389380530973, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=556, Percent_Identity=43.8848920863309, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI21357815, Length=502, Percent_Identity=42.4302788844622, Blast_Score=393, Evalue=1e-109,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59886; Mature: 59755

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEV
CCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKDRVIAGSEGHDIAIVEVGGTVGDIESLPFMEAIRQLAIEVGREHAMFMHLTLVPYL
HHHHHHEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRMIPANERKKIALFCNVPEKAVISMKD
HHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCHHHHEEHHH
VDSIYKIPQLIRSQGLDDLVCARFGINAPEADLSEWEQVIYEEANPTGEVTIGMVGKYTE
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCC
LPDAYKSVNEALKHAGLKNRLSVTIKYVDSQDVETKGTDVLNGLDAILVPGGFGDRGIEG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHCCCCEEEECCCCCCCCCCC
KIRAAQYARENKIPYLGICLGMQVALIEYARNVAGMEGAHSTEFNKNTKYPVVGLITEWV
CHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHC
DGEGNVEERSEKSDLGGTMRLGSQLCHLEKGTKAYELYGSATIHERHRHRYEVNNLLRPQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
IEKAGLKVSGLSADKKLVEVIENPAHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGQFQ
HHHCCCEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
RGELK
CCCCC
>Mature Secondary Structure 
TTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEV
CCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKDRVIAGSEGHDIAIVEVGGTVGDIESLPFMEAIRQLAIEVGREHAMFMHLTLVPYL
HHHHHHEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRMIPANERKKIALFCNVPEKAVISMKD
HHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCHHHHEEHHH
VDSIYKIPQLIRSQGLDDLVCARFGINAPEADLSEWEQVIYEEANPTGEVTIGMVGKYTE
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCC
LPDAYKSVNEALKHAGLKNRLSVTIKYVDSQDVETKGTDVLNGLDAILVPGGFGDRGIEG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHCCCCEEEECCCCCCCCCCC
KIRAAQYARENKIPYLGICLGMQVALIEYARNVAGMEGAHSTEFNKNTKYPVVGLITEWV
CHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHC
DGEGNVEERSEKSDLGGTMRLGSQLCHLEKGTKAYELYGSATIHERHRHRYEVNNLLRPQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
IEKAGLKVSGLSADKKLVEVIENPAHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGQFQ
HHHCCCEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
RGELK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA