Click here to switch to the map view.

The map label for this gene is yneF [H]

Identifier: 147675023

GI number: 147675023

Start: 449582

End: 451156

Strand: Reverse

Name: yneF [H]

Synonym: VC0395_A0422

Alternate gene names: 147675023

Gene position: 451156-449582 (Counterclockwise)

Preceding gene: 147675739

Following gene: 147673433

Centisome position: 14.92

GC content: 47.49

Gene sequence:

>1575_bases
TTGTTGTACATGGGCGTTCTAGAACAAGATATCCAAGCAGAGCTTCAAAAACTGAAATCTCAGTTAGAACAAGTACGTTT
GACACAAAGGGATACTTCGTTCAAATTTAACCGAGAGCAGCAAGTTCTTAAACGAATGGTCACTTCCTTGGCTACGACTT
GCCGCGGCAGTAGCTCTCGACTAAACCAAAGTTTAGAAGAACTCTGTTTTGCATTAGAACAACAAAAAGACATCAGTACG
CTGATCCCGAAATTAGCCGTACTGGAACGCATGTTGAAACAACAGTCATTGGCCATGGACAAACAAACGAGTCATCTGGA
TAGCCAGCTACAGCACAGTGGTGAAACGCTACTGCGCGTCCCCGGCTTACCCGCCAAGATCAAACGCGATCTTCGAGATC
TGCTGAGTTTTAGCGCCGCACAGCCGCTGGAAAAAACTCAGCAAGCTTTGCGTTTGCTGGAAATTTATGAACGCGCGGTC
AAAATTCAATGTGCCAATCCCAATTTGGCGCTCAATGAGCTTACTCAACAAGCGGATCGAGAACTGCTGCATCGACTTGC
CGTTGATTTACAACACTTGATTACTGAGTTGGATTTCAATGGTGAATCGGGTGATCAACTGACCGATATCCGAACCAAGC
TTCTGCTTGGCGTCAATACTCATACCCTGCTTGAGCTCACCCTTGAAGTGCTGCGTTTAGTCGTCGATGGAAGCCATGCT
GAGCGCAAAGCAGCCGAAGCCTTTCTCGAGCAAGTGAATCTCTCGATTGGCAATAACCTAAAAAGCGCGACTCAAAATCT
TGAGCAAAGCCAGAGTTACATGGCACACCGCCAAGAGATGAATGGCGAGCTGAGCAGTTTAGTGGCTCGTAGCCAGACCG
CGCTCAAAGAACAGACGGAGCTAGAACAGCTCAAGCTGAAAATGGCGCCTTTACTGCGCGAGATGTCTTCACTTTCTGAA
CGCTTACTCTTAGCGGAGCAACGTGAGCAAGCCCTACTTGAGCGTATGCGCTATAGCAAAGATCAGATGGAAGCCCTGTC
TGATTTGGCACAAGATTATCGTCGCCGCCTTGAAGATCAAGCATTGCGCGCGCAACTCGATCCTCTGACCAAAGTGTACA
ACCGCAGTAGCTTTACTGAGCGACTTGAACATGAGTATCGCCGCTGGATCCGTACGCAACACAATTTGCGGGTAGTGCTG
TTTGATATTGATAAATTCAAATCGATCAACGACAGCTTTGGCTATACCGCAGGCGATAAGGCCTTAAGTATCATTGCTCG
CACCATCAAAAAAGAATTACGAGACAGTGACACCGTGGCTCGCTTCTCTGGTGAAGAGTTCATTCTGTTACTGCCTGAAC
GCTCCGATAATGAGAGTTACCAGATTATTCACCAGATCCAGCTCAACGTGTCGAAACTACCGTTCAAGTTCCGCGATAAG
AGCCTAACCATCACGCTGTCTGCGGCGAGTATCCGCTTCATGGATTCAGATACCCCCGAAACGGTTCTTGATCGTTTAAA
TCTGACGCTAAGTGAAGCCAAACATATCGGTCCAAGTCAGTTAGCTTGGAAATAA

Upstream 100 bases:

>100_bases
TGCTGCCCAGTCGGCATAAACACTGGGTGACGCCAGCAAAATATTTGTTATTATCGACAAATCAATAAATAGGATCAGGA
TTGATCCTCAACTATCCAGT

Downstream 100 bases:

>100_bases
CTGATTAATATTTAAGCTAATTAACTATTAATTTGTGCTCAATTTCACAATTACCCCCTCACTCTATTTTCTTACCTAAC
TAGATACCTCTAGATTGAAG

Product: GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSRLNQSLEELCFALEQQKDIST
LIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRVPGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAV
KIQCANPNLALNELTQQADRELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTELEQLKLKMAPLLREMSSLSE
RLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVL
FDIDKFKSINDSFGYTAGDKALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLAWK

Sequences:

>Translated_524_residues
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSRLNQSLEELCFALEQQKDIST
LIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRVPGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAV
KIQCANPNLALNELTQQADRELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTELEQLKLKMAPLLREMSSLSE
RLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVL
FDIDKFKSINDSFGYTAGDKALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLAWK
>Mature_524_residues
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSRLNQSLEELCFALEQQKDIST
LIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRVPGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAV
KIQCANPNLALNELTQQADRELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTELEQLKLKMAPLLREMSSLSE
RLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVL
FDIDKFKSINDSFGYTAGDKALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLAWK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=127, Percent_Identity=43.3070866141732, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI87082007, Length=126, Percent_Identity=37.3015873015873, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI145693134, Length=127, Percent_Identity=35.4330708661417, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786584, Length=126, Percent_Identity=34.1269841269841, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=29.4871794871795, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081881, Length=189, Percent_Identity=27.5132275132275, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788381, Length=100, Percent_Identity=39, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081974, Length=101, Percent_Identity=31.6831683168317, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787262, Length=110, Percent_Identity=33.6363636363636, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=175, Percent_Identity=29.7142857142857, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 60196; Mature: 60196

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
LNQSLEELCFALEQQKDISTLIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAVKIQCANPNLALNELTQQADR
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHH
ELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHH
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEQLKLKMAPLLREMSSLSERLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVLFDIDKFKSINDSFGYTAGDK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCHHH
ALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
HHHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCC
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLAWK
EEEEEEEEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
LNQSLEELCFALEQQKDISTLIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAVKIQCANPNLALNELTQQADR
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHH
ELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHH
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEQLKLKMAPLLREMSSLSERLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVLFDIDKFKSINDSFGYTAGDK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCHHH
ALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
HHHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCC
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLAWK
EEEEEEEEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]