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The map label for this gene is glnS

Identifier: 147674963

GI number: 147674963

Start: 553879

End: 555549

Strand: Direct

Name: glnS

Synonym: VC0395_A0517

Alternate gene names: 147674963

Gene position: 553879-555549 (Clockwise)

Preceding gene: 147675085

Following gene: 147673505

Centisome position: 18.32

GC content: 46.5

Gene sequence:

>1671_bases
ATGAGTGAAGCTGAGGCTCGTCCATCGAACTTCATCCGCCAAATTATCGATAAAGATCTTGCGGATGGTAAACACACGAC
CGTGCATACTCGTTTCCCGCCGGAGCCGAATGGCTACCTGCACATCGGTCACGCCAAGTCAATCTGTCTGAATTTCGGTA
TTGCTCAGGATTATCAAGGGCAGTGCAACCTGCGCTTTGATGATACAAACCCAGAAAAAGAGAACCTTGAATATGTTGAG
TCGATCAAAAAAGATGTGACTTGGCTAGGTTTTGATTGGTCAGGTGAGGTGTGTTACTCCTCTGACTATTTTGACAAGTT
GTATGAATACGCGATTGAGCTGATTCAAAAAGGCTTGGCGTATGTGGATGAACTGACGCCTGAGCAAATTCGTGAATACC
GTGGCACTCTAACTGAGCCTGGTAAGCACAGCCCATACCGCGATCGTAGCGTAGAAGAAAACCTAGCGCTGTTTGAAAAA
ATGCGTGCGGGTGAATTTGCAGAAGGTCAAGCTTGTCTACGTGCAAAAATCGATATGGCATCGTCTTTTATCGTAATGCG
TGATCCTGTTTTGTACCGTGTGCGTTTTGCTGAACACCACCAAACGGGTGACAAGTGGTGCATCTATCCGATGTACGATT
TCACACACTGTATTTCTGATGCGCTCGAAGGCATTACGCACTCTATTTGTACTTTGGAATTCCAAGACAACCGCCGTCTG
TATGATTGGGTTCTTGATAACATCACGATTCCATGCCATCCACGCCAGTATGAATTCAGCCGCTTAAATCTCGAATATAC
CGTGATGTCTAAGCGTAAACTGAACCAGCTAGTGACCGAAAAATTGGTCACAGGTTGGGATGATCCACGTATGCCGACCA
TTTCAGGCCTGCGTCGCCGTGGCTTCACGCCAAGCGCTATTCGTGAATTCTGTAAGCGCATTGGTGTGACTAAGCAAGAA
AACATGATTGAATACAGTGCATTAGAGTCTTGTATTCGTGATGATCTTAACGAAAATGCGCCACGTGCGATGGCCGTTCT
GGATCCGGTTAAACTGGTGATTGAGAACTTCGCCGCAGGCACAGTGGAAACTTTGACGCTGGCTAACCATCCGAACAAAC
CTGAGATGGGCGATCGCGAAGTGCCATTTACCCGTGAGTTGTGGATTGAACGCGAAGATTTCCGCGAAGAAGCCAACAAG
AAATACAAGCGTTTGGTGTTAGGCAAAGAAGTGCGTCTGCGTGGTGCTTACGTCATCAAAGCTGAGCGCATCGAAAAAGA
TGAACAAGGTAATATCACCACAATCTTCTGTTCTTACGATCCTGAAACCTTGGGTAAAAACCCAGCCGATGGTCGTAAAG
TGAAAGGTGTTATTCACTGGGTATCTGCAGAAAAAGGCGTTCCTGCTGAGTTTCGTCTCTACGAGCGTCTGTTCACTGTA
CCCAATCCAGGTGCGGCGGATAACTTTGCCGAAACCATTAACCCAGAATCTCTGGTAAAAGTTCAAGGTTATGTTGAGCC
AAGCTTAGTGGAAGCAAAACCTGAATTTGGTTATCAGTTTGAGCGTATGGGCTATTTCTGTGCCGATAACAAAGACTCTA
GCCCGCAGGCTTTGGTCTTTAACCGCACTGTTGGTTTGCGCGATAGTTTCGTCAAAATTGACGAAGAGTAA

Upstream 100 bases:

>100_bases
TGTGGATGATTAGCGGCAATATTTTCTGTTTTCTAGCCTTGATGGTACAAGAAGCAGAAACGCTTTCTGACAATACTAAA
TGAATTTTGAGGTGCTATAG

Downstream 100 bases:

>100_bases
GCCGAAAAACGAGAGTAAAATGCAAAGCCAGCCAACGTGCTGGCTTTGTTTTTAGGCGTGGAGAAAGCGGTGTTGCCTCA
CGCTAGGACTGAAATTTGTG

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKENLEYVE
SIKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLAYVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEK
MRAGEFAEGQACLRAKIDMASSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
YDWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRRGFTPSAIREFCKRIGVTKQE
NMIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAGTVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANK
KYKRLVLGKEVRLRGAYVIKAERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTV
PNPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVFNRTVGLRDSFVKIDEE

Sequences:

>Translated_556_residues
MSEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKENLEYVE
SIKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLAYVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEK
MRAGEFAEGQACLRAKIDMASSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
YDWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRRGFTPSAIREFCKRIGVTKQE
NMIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAGTVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANK
KYKRLVLGKEVRLRGAYVIKAERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTV
PNPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVFNRTVGLRDSFVKIDEE
>Mature_555_residues
SEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKENLEYVES
IKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLAYVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEKM
RAGEFAEGQACLRAKIDMASSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRLY
DWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRRGFTPSAIREFCKRIGVTKQEN
MIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAGTVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANKK
YKRLVLGKEVRLRGAYVIKAERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTVP
NPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVFNRTVGLRDSFVKIDEE

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI4826960, Length=532, Percent_Identity=47.3684210526316, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI62241042, Length=538, Percent_Identity=33.8289962825279, Blast_Score=282, Evalue=4e-76,
Organism=Escherichia coli, GI1786895, Length=555, Percent_Identity=80.7207207207207, Blast_Score=953, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=318, Percent_Identity=22.6415094339623, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=532, Percent_Identity=43.609022556391, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17511007, Length=511, Percent_Identity=34.4422700587084, Blast_Score=261, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6324742, Length=565, Percent_Identity=43.1858407079646, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321192, Length=526, Percent_Identity=35.171102661597, Blast_Score=306, Evalue=5e-84,
Organism=Drosophila melanogaster, GI17864488, Length=527, Percent_Identity=43.4535104364326, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24649466, Length=515, Percent_Identity=34.368932038835, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYQ_VIBC3 (A5F2T1)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F2T1
- SMR:   A5F2T1
- STRING:   A5F2T1
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0517
- eggNOG:   COG0008
- HOGENOM:   HBG334108
- OMA:   RMPTIAG
- ProtClustDB:   PRK05347
- BioCyc:   VCHO345073:VC0395_A0517-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00126
- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119:SF3
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00440

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.18

Molecular weight: Translated: 64118; Mature: 63987

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQG
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCHHEEHEECCCCCCCCC
QCNLRFDDTNPEKENLEYVESIKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLA
CEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
YVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEKMRAGEFAEGQACLRAKIDMA
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
CCEEEEECCEEEEEEEHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
YDWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRR
HHHHHHCCEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GFTPSAIREFCKRIGVTKQENMIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAG
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
TVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANKKYKRLVLGKEVRLRGAYVIK
CEEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECEEEEEE
AERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTV
HHHCCCCCCCCEEEEEECCCHHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCC
PNPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVF
CCCCCCCHHHHHCCCHHEEEEECCCCCHHCCCCCCCCCCHHHCCEEEECCCCCCCCEEEE
NRTVGLRDSFVKIDEE
ECCCCCCCCCEEECCC
>Mature Secondary Structure 
SEAEARPSNFIRQIIDKDLADGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQG
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCHHEEHEECCCCCCCCC
QCNLRFDDTNPEKENLEYVESIKKDVTWLGFDWSGEVCYSSDYFDKLYEYAIELIQKGLA
CEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
YVDELTPEQIREYRGTLTEPGKHSPYRDRSVEENLALFEKMRAGEFAEGQACLRAKIDMA
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SSFIVMRDPVLYRVRFAEHHQTGDKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
CCEEEEECCEEEEEEEHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
YDWVLDNITIPCHPRQYEFSRLNLEYTVMSKRKLNQLVTEKLVTGWDDPRMPTISGLRRR
HHHHHHCCEECCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GFTPSAIREFCKRIGVTKQENMIEYSALESCIRDDLNENAPRAMAVLDPVKLVIENFAAG
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
TVETLTLANHPNKPEMGDREVPFTRELWIEREDFREEANKKYKRLVLGKEVRLRGAYVIK
CEEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECEEEEEE
AERIEKDEQGNITTIFCSYDPETLGKNPADGRKVKGVIHWVSAEKGVPAEFRLYERLFTV
HHHCCCCCCCCEEEEEECCCHHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCC
PNPGAADNFAETINPESLVKVQGYVEPSLVEAKPEFGYQFERMGYFCADNKDSSPQALVF
CCCCCCCHHHHHCCCHHEEEEECCCCCHHCCCCCCCCCCHHHCCEEEECCCCCCCCEEEE
NRTVGLRDSFVKIDEE
ECCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA